Molecular cloning : a laboratory manual

Published on Jan 1, 2001
David W. Russell19
Estimated H-index: 19
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Abstract
馃摉 Papers frequently viewed together
2000
1 Author (Tobias. Kieser)
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Cited By19238
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#1Tetsuo Mioka (Hokkaido University)H-Index: 5
#2Tian Guo (Hokkaido University)H-Index: 1
Last. Kazuma Tanaka (Hokkaido University)H-Index: 29
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Membrane phase separation to form micron-scale domains of lipids and proteins occurs in artificial membranes; however, a similar large-scale phase separation has not been reported in the plasma membrane of the living cells. We show here that a stable micron-scale protein-depleted region is generated in the plasma membrane of yeast mutants lacking phosphatidylserine at high temperatures. We named this region the 'void zone'. Transmembrane proteins and certain peripheral membrane proteins and phos...
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#1T.G. Sumithra (CMFRI: Central Marine Fisheries Research Institute)H-Index: 5
#2S R Krupesha Sharma (CMFRI: Central Marine Fisheries Research Institute)H-Index: 7
Last. Sujitha Thomas (ICAR: Indian Council of Agricultural Research)H-Index: 1
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Abstract null null Red sea bream iridovirus (RSIV) is an emerging viral pathogen having significant socio-economic and environmental impacts on marine and brackish aquaculture systems. The study documents certain interesting findings of an RSIV disease outbreak that caused 50% cumulative mortality among cage farmed Asian sea bass in India. The confirmatory diagnosis was made based on internationally approved protocols. The affected fish showed splenomegaly, anaemia, and abnormally enlarged cells...
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#1Zoe Giagkazoglou (University of the Aegean)
#2Andrew M. Griffiths (University of Exeter)H-Index: 21
Last. Ioannis E. Batjakas (University of the Aegean)H-Index: 8
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Abstract null null Mislabeling of seafood products and marketing of protected species remains a worldwide issue despite the labeling regulations set at a local, European and International level. DNA barcoding has proven to be the most popular and accurate method of detection of fraudulent seafood products. This study investigated the batoid meat market of Greece, the mislabeling rates and the protected species occurrence. A total of 114 ray products were collected from fishmongers, open markets,...
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#1Jessica Aparecida Ferrarezi (USP: University of S茫o Paulo)
#2Paula de Almeida Carvalho-Estrada (USP: University of S茫o Paulo)H-Index: 4
Last. Jo茫o L煤cio de Azevedo (USP: University of S茫o Paulo)H-Index: 55
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Abstract null null The objective of this study was to investigate the effects of the inoculation with the plant growth-promoting rhizobacteria Bacillus thuringiensis RZ2MS9 and Burkholderia ambifaria RZ2MS16, both from the Brazilian Amazon, on the bacterial community of the rhizobiome and leaves of maize grown in field. For comparison, we analysed the effects of inoculating Azospirillum brasilense Ab-V5, a strain that is commercialized as inoculant for maize, as well as the combinations RZ2MS16聽...
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#1Edilane Cristina do Nascimento (UFJF: Universidade Federal de Juiz de Fora)
#2Melissa Correa Sabino (UFJF: Universidade Federal de Juiz de Fora)
Last. Humberto Moreira H煤ngaro (UFJF: Universidade Federal de Juiz de Fora)H-Index: 6
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Abstract null null In this study, P. fluorescens-infecting phages were isolated, characterized, and evaluated to their potential to control the bacterial counts and, consequently, the proteolytic spoilage of raw milk during cold storage. The UFJF_PfDIW6 and UFJF_PfSW6 phages showed titers of 9.7 and 7.6 log PFU/ml; latent period of 115 and 25鈥痬in, and burst size of 145 and 25鈥疨FU/infected cell, respectively. They also were highly specific to the host bacterium, morphologically classified as the ...
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#1Bijay Kumar Behera (ICAR: Indian Council of Agricultural Research)H-Index: 20
#2Prasenjit Paria (ICAR: Indian Council of Agricultural Research)H-Index: 9
Last. Basanta Kumar Das (ICAR: Indian Council of Agricultural Research)H-Index: 27
view all 4 authors...
Abstract null null The present research study was carried out to investigate the causative agent responsible for infection and mortality of farmed Labeo rohita. Samples of infected fishes were collected from aquaculture farms of West Bengal, India. Biochemical and molecular identification revealed that isolated strain EM4 was Citrobacter freundii. Furthermore, a challenge study was carried out to determine the number of bacterial colonies required for 50% mortality (LD50) of the experimental fis...
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#1Manoharmayum Shaya Devi (ICAR: Indian Council of Agricultural Research)
#2Prasenjit Paria (ICAR: Indian Council of Agricultural Research)H-Index: 9
Last. Basanta Kumar Das (ICAR: Indian Council of Agricultural Research)H-Index: 27
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Abstract null null Vibrio cholerae is a gram-negative bacteria known for causing epidemics and pandemics conditions, comprised of both non-pathogenic and pathogenic strains. The present study identified a non O1, non-O139 V. cholerae (EMM1) serotype isolated from diseased Labeo rohita cultured in a wetland system at Purba Medinipur district, West Bengal, India. Identification of the isolate was done by biochemical test, 16S rRNA gene sequencing and phylogenetic analysis. The in vivo challenge ex...
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#1Elo铆sa Torres-Hern谩ndez (UNAM: National Autonomous University of Mexico)H-Index: 1
#2Isai Betancourt-Resendes (Autonomous University of Queretaro)H-Index: 1
Last. Omar Dom铆nguez-Dom铆nguez (UMSNH: Universidad Michoacana de San Nicol谩s de Hidalgo)H-Index: 18
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Abstract null null Marine species that are widely distributed in the Tropical Eastern Pacific (TEP) has served as a model for studying biogeographic patterns resulting from the effects of intraregional habitat discontinuities and oceanographic processes on the diversification and evolution of cryptobenthic reef fishes. Tomicodon petersii, a clingfish (Gobiesocidae) endemic to the TEP, is found on very shallow rocky reefs from central Mexico to northern Peru, and in the Cocos and Galapagos island...
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#1Madjid Delghandi (Sultan Qaboos University)H-Index: 13
#2Marit Delghandi (Sultan Qaboos University)H-Index: 1
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Genetic markers are widely applied in the study of genetic diversity for many species. The approach incorporates a Polymerase Chain Reaction (PCR) amplification of targeted sequences in the genome. Crucial for the overall success of a PCR experiment is the careful design of synthetic oligonucleotide primers. Ideally designed primer pairs will ensure the efficiency and specificity of the amplification reaction, resulting in a high yield of the desired amplicon. Important criteria such as primer-s...
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#1Mitesh Dwivedi (Uka Tarsadia University)H-Index: 19
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