Effect of Hyperin and Icariin on steroid hormone secretion in rat ovarian granulosa cells

Published on Aug 1, 2019in Clinica Chimica Acta2.615
· DOI :10.1016/J.CCA.2018.05.004
Xiaowei Nie1
Estimated H-index: 1
(NU: Nanjing University),
Wenjie Sheng1
Estimated H-index: 1
(NU: Nanjing University)
+ 3 AuthorsYong Tan1
Estimated H-index: 1
(NU: Nanjing University)
Abstract Aim of the study This study was designed to investigate the effect of different concentrations of Hyperin and Icariin (ICA)on proliferation and the secretion of estrogen (E2), and progesterone (P) in granulosa cells, and to explore the effect of Hyperin and Icariin on the expression of CYP17 and CYP19 . Materials and methods Rat ovary granulosa cells were cultured in vitro and treated with different concentrations of Hyperin and Icariin. The proliferation of ovarian granulosa cells was measured with the MTT assay. The concentration of estradiol was measured with a magnetic particle-based enzyme-linked immunosorbent assay (ELISA) kit. The CYP17 and CYP19 mRNA expression was detected by quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). The CYP17 and CYP19 protein expression was determined with Western blotting. Results Hyperin (50 μg/l) and Icariin (10 μg/l) significantly increased proliferation of ovarian granulosa cells and secretion of estrogen and progesterone. Hyperin and Icariin stimulated the mRNA and protein expression of CYP17 and CYP19. Conclusions These results showed that Hyperin and Icariin can promote the secretion of E2 and P through up-regulation of CYP17 and CYP19. Frequently used Chinese herbs like Cuscuta Chinensis Lam and Epimedium Brevicornu maxim, which contain Hyperin and Icariin, could improve the ovarian endocrine function through these effects.
📖 Papers frequently viewed together
7 Citations
8 Citations
11 Citations
#1Julie Ducharme (McGill University)H-Index: 10
#2Karine Auclair (McGill University)H-Index: 25
Abstract Bioconjugation, defined as chemical modification of biomolecules, is widely employed in biological and biophysical studies. It can expand functional diversity and enable applications ranging from biocatalysis, biosensing and even therapy. This review summarizes how chemical modifications of cytochrome P450 enzymes (P450s or CYPs) have contributed to improving our understanding of these enzymes. Genetic modifications of P450s have also proven very useful but are not covered in this revie...
6 CitationsSource
#1Hong-Jun Kang (Yunnan University)H-Index: 1
#2Xingqing Xiao (NCSU: North Carolina State University)H-Index: 7
Last. Xianghui Zeng (Yunnan University)H-Index: 20
view all 7 authors...
Abstract Estrogen is a significant factor in the maintenance and progression of hormone-dependent breast cancer. As well known, aromatase mediates the production of estrogen. Thus, inhibition of aromatase with chemical molecules has been considered to be an effective treatment for estrogen receptor-positive (ER+) breast cancer. In this work, we designed and synthesized a series of novel non-steroidal molecules containing 2-phenylindole scaffold and moiety of either imidazole or 1,2,4-triazole to...
27 CitationsSource
#1Monika Kmeťová Sivoňová (Comenius University in Bratislava)H-Index: 7
#2Jana JurečekováH-Index: 6
Last. Jozef HatokH-Index: 14
view all 6 authors...
: Androgens play an important role during the development of both normal prostate epithelium and prostate cancer and variants of genes involved in androgen metabolism may be related to an increased risk of prostate disease. Cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17A1) is a key regulatory enzyme in the steroidogenic pathway; it catalyses both 17α-hydroxylase and 17,20-lyase activities and is essential for the production of both androgens and glucocorticoids. In this review, we focus on t...
14 CitationsSource
#1Jinjin Zhang (HUST: Huazhong University of Science and Technology)H-Index: 11
#2Li Fang (HUST: Huazhong University of Science and Technology)H-Index: 6
Last. Shixuan Wang (HUST: Huazhong University of Science and Technology)H-Index: 31
view all 9 authors...
Abstract Ethnopharmacological relevance Kuntai capsule, a traditional Chinese medicine, has been widely used for the clinical treatment of menopausal syndrome. However, its mechanisms remain poorly understood. Considering that aging ovaries are the primary cause of menopause, this study was designed to investigate the effects and mechanisms of Kuntai capsule on ovarian function in a novel mice model with accelerated aging ovaries. Materials and methods Seventy-five female C57BL/6 mice were chose...
11 CitationsSource
#1Yves MenezoH-Index: 44
#2Erica SilvestrisH-Index: 11
Last. Kay Elder (Bourn Hall Clinic)H-Index: 26
view all 4 authors...
Abstract The negative effect of oxidative stress on the human reproductive process is no longer a matter for debate. Oxidative stress affects female and male gametes and the developmental capacity of embryos. Its effect can continue through late stages of pregnancy. Metabolic disorders and psychiatric problems can also be caued by DNA methylation and epigenetic errors. Age has a negative effect on oxidative stress and DNA methylation, and recent observations suggest that older men are at risk of...
84 CitationsSource
#1Liming Xue (UBC: University of British Columbia)H-Index: 8
#2Yi-Ping Jiang ('SMMU': Second Military Medical University)H-Index: 16
Last. Qiao-Yan Zhang ('SMMU': Second Military Medical University)H-Index: 32
view all 7 authors...
Abstract Ethnopharmacological relevance Icariin, a principal flavonoid glycoside of Epimedium brevicornu Maxim, has been widely proved to possess antiosteoporotic activity with promoting bone formation and decreasing bone resorption. However, the involving mechanisms remain unclear. Aim of the study To clear a global insight of signal pathways involved in anti-osteoporotic mechanism of icariin at proteins and metabolites level by integrating the proteomics and NMR metabonomics, in a systems biol...
17 CitationsSource
#1Nan ZongH-Index: 1
#2Fei LiH-Index: 13
Last. Qi-Hai Gong (Zunyi Medical College)H-Index: 21
view all 6 authors...
Abstract Excitotoxicity is one of the most extensively studied causes of neuronal death and plays an important role in Alzheimer’s disease (AD). Icariin is a flavonoid component of a traditional Chinese medicine reported to possess a broad spectrum of pharmacological effects. The present study was designed to investigate the effects of icariin against learning and memory impairment induced by excitotoxicity. Here, we demonstrated that rats receiving intracerebroventricular injection of excitator...
13 CitationsSource
#1Liangyan Shi (HUST: Huazhong University of Science and Technology)H-Index: 6
#2Jinjin Zhang (HUST: Huazhong University of Science and Technology)H-Index: 11
Last. Shixuan Wang (HUST: Huazhong University of Science and Technology)H-Index: 31
view all 10 authors...
: Ovarian aging is a long-term and complex process associated with a decrease in follicular quantity and quality. The damaging effects of reactive oxygen species (ROS) in ovarian aging and ovarian aging-associated disorders have received relatively little attention. Thus, we assessed if the oxidative stress induced by long-term (defined by the Environmental Protection Agency as at least 30 days in duration) moderate ozone inhalation reduced ovarian reserves, decreased ovarian function and induce...
24 CitationsSource
#1Ming Wei Lin (NYMU: National Yang-Ming University)H-Index: 4
#2Meng Hsing Wu (NCKU: National Cheng Kung University)H-Index: 31
Vitamin D plays a physiologic role in reproduction including ovarian follicular development and luteinization via altering anti-mullerian hormone (AMH) signalling, follicle-stimulating hormone sensitivity and progesterone production in human granulosa cells1. It also affects glucose homeostasis through manifold roles. The potential influences of vitamin D on glucose homeostasis include the presence of specific vitamin D receptor (VDR) in pancreatic β-cells and skeletal muscle, the expression of ...
9 CitationsSource
#1Xiaomei Zhang (YZU: Yangzhou University)H-Index: 3
#2Fang Lv (MOE: Chinese Ministry of Education)H-Index: 3
Last. Rong HuH-Index: 1
view all 10 authors...
Meta-analyses have found conflicting results with respect to the use of progesterone or progesterone plus estrogen as luteal phase support for in vitro fertilization (IVF) protocols involving gonadotropins and/or gonadotropin-releasing hormone analogs. The aim of the present study was to perform an updated meta-analysis on the efficacy of progesterone versus progesterone plus estrogen as luteal phase support. We searched the MEDLINE, Cochrane Library, and Google Scholar databases (up to March 18...
10 CitationsSource
Cited By7
#1Shuying Huo (Agricultural University of Hebei)H-Index: 3
#2Yurong Li (Agricultural University of Hebei)H-Index: 3
Last. Peipei Gao (Agricultural University of Hebei)
view all 6 authors...
Abstract In the present study, for the purpose of investigating the effects of the total flavonoids of Epimedium (TFE) in regard to preventing the development of atrophied oviducts and follicles induced by forced molting, 300-day-old Hy-Line Brown layer hens were divided into 3 study groups as follows: the control (CON) group was the normal group, without forced molting and TFE treatments; the TFE1 group was treated by adding a 1‰ TFE treatment after forced molting; and the TFE0 group was not tr...
#1Bo DuH-Index: 2
#2Lihong LiuH-Index: 2
Last. Hao AiH-Index: 2
view all 4 authors...
Objective To predict the chemical compositions and drug targets and to systematically dissect the pharmacological mechanism of Erxian Decoction (二仙汤, EXD) as a treatment for premature ovarian failure (POF) using a systems pharmacology approach.
2 CitationsSource
#1Chunyang HeH-Index: 2
#2Ze WangH-Index: 2
Last. Jing-Shan ShiH-Index: 2
view all 3 authors...
Abstract Icariin (ICA) is a principal active component from traditional Chinese medicine Epimedium grandiflorum. To explain its traditional medical usages by modern science, a variety of pharmacological effects have been studied for ICA. In this review, we summarized the pharmacokinetics of ICA as well as its pharmacological mechanisms in neurodegenerative disease, cardiovascular disease, anti-osteoporosis, anti-inflammation, anti-oxidative stress, anti-depression and anti-tumors.
8 CitationsSource
#1Ning Li (Qingdao University)H-Index: 2
#2Jing Wang (Qingdao University)H-Index: 1
Last. Zenghui Li (Qingdao University)H-Index: 2
view all 5 authors...
Abstract Icariin is one of the most common active ingredients in traditional Chinese medicine, while its function against Premature ovarian failure (POF) has not been explored. POF animal model was induced by d -galactose, and icariin at different doses was administered. Ovarian structure and follicle counting were observed via hematoxylin and eosin staining. The levels of serum hormones were measured by ELISA. Primary ovarian granulosa cells were cultured to compare the protective effects of ic...
6 CitationsSource
#1Yuping Shen (Jiangsu University)H-Index: 7
#2Man Wang (Jiangsu University)H-Index: 3
Last. Xiaobin Jia (Jiangsu University)H-Index: 8
view all 12 authors...
ABSTRACT Baohuoside I, a rare secondary glycoside from Epimedii Folium, has anti-osteoporosis and other pharmacological effects. In addition, it exhibits improved bioactivities compared with its original glycoside icariin. Conventional methods for the production of baohuoside I, such as acid hydrolysis, exhibit very low efficiency with serious pollution and substantial byproducts. The aim of this study was to develop an eco-efficient biphasic enzymatic hydrolysis system for the green production ...
3 CitationsSource
#1Huishan Zhao (Qingdao University)H-Index: 8
#2Yinghua Shan (Qingdao University)H-Index: 2
Last. Benjiao Gong (Qingdao University)H-Index: 4
view all 5 authors...
Background and purpose Premature ovarian insufficiency (POI) refers to a hypergonadotropic hypoestrogenism and the condition of pre-onset ovarian function failure. Epimedium is a common traditional Chinese herbal medicine that is widely used to relieve POI in China. To systematically explore the pharmacological mechanism of epimedium on POI therapy, a network pharmacology approach was conducted at the molecular level.
5 CitationsSource
#1Xiaochuan Zheng (NAU: Nanjing Agricultural University)H-Index: 4
#2Cheng Chi (NAU: Nanjing Agricultural University)H-Index: 11
Last. Wen-Bin Liu (NAU: Nanjing Agricultural University)H-Index: 28
view all 11 authors...
Abstract We investigated the effects of icariin (ICA) on growth performance, antioxidant capacity and non-specific immunity in Chinese mitten crab (Eriocheir sinensis). A total of 200 healthy crabs (average weight: 33.58 ± 0.05 g) were randomly assigned to four treatments with five replicates, each with ten individuals per pool. There were four dietary treatments: the control group (fed with the basal diet), the ICA 50 group, the ICA100 group, and the ICA 200 group (fed with the basal diet suppl...
18 CitationsSource