Effect of Hyperin and Icariin on steroid hormone secretion in rat ovarian granulosa cells

Published on Aug 1, 2019in Clinica Chimica Acta2.615
· DOI :10.1016/J.CCA.2018.05.004
Xiaowei Nie1
Estimated H-index: 1
(NU: Nanjing University),
Wenjie Sheng1
Estimated H-index: 1
(NU: Nanjing University)
+ 3 AuthorsYong Tan1
Estimated H-index: 1
(NU: Nanjing University)
Sources
Abstract
Abstract Aim of the study This study was designed to investigate the effect of different concentrations of Hyperin and Icariin (ICA)on proliferation and the secretion of estrogen (E2), and progesterone (P) in granulosa cells, and to explore the effect of Hyperin and Icariin on the expression of CYP17 and CYP19 . Materials and methods Rat ovary granulosa cells were cultured in vitro and treated with different concentrations of Hyperin and Icariin. The proliferation of ovarian granulosa cells was measured with the MTT assay. The concentration of estradiol was measured with a magnetic particle-based enzyme-linked immunosorbent assay (ELISA) kit. The CYP17 and CYP19 mRNA expression was detected by quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). The CYP17 and CYP19 protein expression was determined with Western blotting. Results Hyperin (50 μg/l) and Icariin (10 μg/l) significantly increased proliferation of ovarian granulosa cells and secretion of estrogen and progesterone. Hyperin and Icariin stimulated the mRNA and protein expression of CYP17 and CYP19. Conclusions These results showed that Hyperin and Icariin can promote the secretion of E2 and P through up-regulation of CYP17 and CYP19. Frequently used Chinese herbs like Cuscuta Chinensis Lam and Epimedium Brevicornu maxim, which contain Hyperin and Icariin, could improve the ovarian endocrine function through these effects.
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