Acetic acid/1-ethyl-3-methylimidazolium acetate as a biphasic solvent system for altering the aggregation behavior of collagen molecules

A novel acetic acid/1-ethyl-3-methylimidazolium acetate (AA/[EMIM][Ac]) biphasic solvent system was adopted to alter the aggregation behavior of collagen molecules. Collagen aggregates (0.5 mg/mL) were investigated in the presence of AA/[EMIM][Ac] at various ratios (10:0 to 5:5). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the triple helical structural integrity was maintained integrity across the whole...