A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation

Giant vesicles (GVs) are commonly used as artificial membranes and microcompartments in biochemical and biophysical studies. The efficiency and/or yield of their production can be strongly affected by the conditions and procedures used, and thus many protocols have been developed. A reverse-phase protocol has been used to prepare liposomes for decades, and is reported to be suitable for preparing GVs. By revisiting a reverse-phase (W/O/W...