Quantifying Binding-Induced Conformational Changes of Proteins using Hydrodynamic Protein Size Measurements

We present a novel method for the measurement of the Stokes radii of proteins, and demonstrate how this approach can be used to detect analyte binding-induced conformational changes in proteins. The proteins of interest are immobilized onto DNA-functionalized gold microelectrodes on the biosensor surface (using generic conjugation methods). By applying alternating electric fields to the microelectrodes, the fluorescently labeled and...