Imaging of native transcription factors and histone phosphorylation at high resolution in live cells

Volume: 217, Issue: 4, Pages: 1537 - 1552
Published: Feb 12, 2018
Abstract
Fluorescent labeling of endogenous proteins for live-cell imaging without exogenous expression of tagged proteins or genetic manipulations has not been routinely possible. We describe a simple versatile antibody-based imaging approach (VANIMA) for the precise localization and tracking of endogenous nuclear factors. Our protocol can be implemented in every laboratory allowing the efficient and nonharmful delivery of organic dye-conjugated...
Paper Details
Title
Imaging of native transcription factors and histone phosphorylation at high resolution in live cells
Published Date
Feb 12, 2018
Volume
217
Issue
4
Pages
1537 - 1552
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