Profiling and analysis of multiple constituents in Baizhu Shaoyao San before and after processing by stir-frying using UHPLC/Q-TOF-MS/MS coupled with multivariate statistical analysis.

Published on Apr 15, 2018in Journal of Chromatography B3.004
· DOI :10.1016/J.JCHROMB.2018.03.003
Yangyang Xu4
Estimated H-index: 4
(Nanjing University of Chinese Medicine),
Hao Cai12
Estimated H-index: 12
(Nanjing University of Chinese Medicine)
+ 10 AuthorsLin Shen3
Estimated H-index: 3
(Nanjing University of Chinese Medicine)
Abstract Baizhu Shaoyao San (BSS) is a famous traditional Chinese medicinal formula widely used for the treatment of painful diarrhea, intestinal inflammation, and diarrhea-predominant irritable bowel syndrome. According to clinical medication, three medicinal herbs (Atractylodis Macrocephalae Rhizoma, Paeoniae Radix Alba, and Citri Reticulatae Pericarpium) included in BSS must be processed using some specific methods of stir-frying. On the basis of the classical theories of traditional Chinese medicine, the therapeutic effects of BSS would be significantly enhanced after processing. Generally, the changes of curative effects mainly result from the variations of inside chemical basis caused by the processing procedure. To find out the corresponding changes of chemical compositions in BSS after processing and to elucidate the material basis of the changed curative effects, an optimized ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry in positive and negative ion modes coupled with multivariate statistical analyses were developed. As a result, a total of 186 compounds were ultimately identified in crude and processed BSS, in which 62 marker compounds with significant differences between crude and processed BSS were found by principal component analysis and t-test. Compared with crude BSS, the contents of 23 compounds were remarkably decreased and the contents of 39 compounds showed notable increase in processed BSS. The transformation mechanisms of some changed compounds were appropriately inferred from the results. Furthermore, compounds with extremely significant differences might strengthen the effects of the whole herbal formula.
📖 Papers frequently viewed together
4 Citations
3 Citations
29 Citations
#1Zunli Ke (SWU: Southwest University)H-Index: 2
#2Ying Yang (SWU: Southwest University)H-Index: 1
Last. Zhiqin Zhou (SWU: Southwest University)H-Index: 1
view all 4 authors...
China is the most important origin center of the genus Citrus L., especially the wild mandarin (Citrus reticulata Blanco). The aim of this work was to systematically qualitative and quantitative analysis of polymethoxylated flavonoids (PMFs) in seven Chinese wild mandarin citrus using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) and UPLC-photodiode array (UPLC-PDA) analyses. This method was validated including the linearity, ...
14 CitationsSource
#1Tingting Zhu (Nanjing University of Chinese Medicine)H-Index: 6
#2Li Wu (Nanjing University of Chinese Medicine)H-Index: 18
Last. Bao Chang CaiH-Index: 2
view all 8 authors...
Abstract Prepared rhubarb is obtained by steaming raw rhubarb with glutinous rice wine. To explore the chemical material basis and functional mechanism of prepared rhubarb, an ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry coupled with gray correlation analysis statistical method was developed. Finally, 19 compounds in rat plasma were detected after oral administration of prepared rhubarb decoction. It was found that 9 of them were the original form of compou...
13 CitationsSource
#1Tolgahan Kocadağlı (Hacettepe University)H-Index: 14
#2Vural Gökmen (Hacettepe University)H-Index: 62
The study describes the kinetics of the formation and degradation of α-dicarbonyl compounds in glucose/wheat flour system heated under low moisture conditions. Changes in the concentrations of glucose, fructose, individual free amino acids, lysine and arginine residues, glucosone, 1-deoxyglucosone, 3-deoxyglucosone, 3,4-dideoxyglucosone, 5-hydroxymethyl-2-furfural, glyoxal, methylglyoxal and diacetyl concentrations were determined to form a multiresponse kinetic model for isomerisation and degra...
40 CitationsSource
#1Yu Duan (Nanjing University of Chinese Medicine)H-Index: 6
#2Ke Pei (Nanjing University of Chinese Medicine)H-Index: 9
Last. Baochang Cai (Nanjing University of Chinese Medicine)H-Index: 24
view all 11 authors...
The approach to investigate traditional Chinese medicine (TCM) is still in its infancy and has been facing enormous challenge. In this paper, a generally applicable strategy was developed for investigation on TCM systematically with an introduced interesting idea about a novel research system which called subchemome. A representative herb-pair, Astragali Radix-Fructus Corni, was successfully employed to expound this novel strategy. Firstly, subchemomes were prepared individually by applying the ...
6 CitationsSource
#1Liu-Qing WuH-Index: 1
#2Yu LiH-Index: 1
Last. Jun Li (WMU: Wenzhou Medical College)H-Index: 1
view all 7 authors...
We measured anti-nociceptive activity of prim-o-glucosylcimifugin (POG), a molecule from Saposhnikovia divaricate (Turcz) Schischk. Anti-nociceptive or anti-inflammatory effects of POG on a formalin-induced tonic nociceptive response and a complete Freund’s adjuvant (CFA) inoculation-induced rat arthritis pain model were studied. Single subcutaneous injections of POG produced potent anti-nociception in both models that was comparable to indomethacin analgesia. Anti-nociceptive activity of POG wa...
10 CitationsSource
#1Jing ChenH-Index: 1
#2XiaLiuH-Index: 1
Last. Deqiang DouH-Index: 1
view all 3 authors...
6 CitationsSource
#1Jun-Xiang RuanH-Index: 7
#2Zhi-heng SuH-Index: 1
Last. Hua ZhengH-Index: 7
view all 6 authors...
Objective: To investigate the chemical constituents changes of Citrus reticulata pericarp before and after processing with vinegar,and to provide scientific basis for the study of active components in Citrus reticulata pericarp. Methods: Citrus reticulata pericarp was processed with vinegar according to the Zhongyao Paozhixue Cidian. The extracts of unprocessed and processed Citrus reticulata pericarp were detected by HPLC. Partial least squares-discriminate analysis( PLS-DA) was employed to rev...
1 Citations
#1Barbara LaddomadaH-Index: 13
#2Sofia CarettoH-Index: 17
Last. Giovanni MitaH-Index: 31
view all 3 authors...
Wheat bran is generally considered a byproduct of the flour milling industry, but it is a great source of fibers, minerals, and antioxidants that are important for human health. Phenolic acids are a specific class of wheat bran components that may act as antioxidants to prevent heart disease and to lower the incidence of colon cancer. Moreover, phenolic acids have anti-inflammatory properties that are potentially significant for the promotion of gastrointestinal health. Evidence on the beneficia...
55 CitationsSource
#1Gang Cao (ZJNU: Zhejiang Chinese Medical University)H-Index: 20
#2Xiaocheng Chen (WMU: Wenzhou Medical College)H-Index: 5
Last. Hongyan ZhangH-Index: 2
view all 5 authors...
This study was conducted to reveal the relation between herbal medicine Fufang decoction and a single drug in terms of material base. Da-Cheng-Qi decoction (DCQD) was used as a model. Ultrahigh-pressure liquid chromatography coupled with a hybrid linear ion trap–high-resolution mass spectrometry (UHPLC-LTQ-Orbitrap) was applied to detect and identify the main chemical compounds. This technique was also employed to determine the different chemical components. Under optimized liquid chromatography...
5 CitationsSource
#1Xinfeng ZhaoH-Index: 11
#2Xiaohui ZhengH-Index: 16
Last. Jianbin ZhengH-Index: 35
view all 6 authors...
26 Citations
Cited By28
Abstract null null The study aimed to investigate the similarities and differences in the chemical constituents and biological activities between wild and cultivated Corydalis saxicola Bunting. Corydalis saxicola is a rare traditional medicine and is mainly distributed in southwest China. The plant is used for treating inflammation, pain, tumors, and other diseases. The extractive process was optimized and chemical compositions, total alkaloid content, antioxidant, anti-inflammatory, and anti-pr...
#1Danjie Zhang (SDUTCM: Shandong University of Traditional Chinese Medicine)
#2Linlin Sun (SDUTCM: Shandong University of Traditional Chinese Medicine)H-Index: 2
Last. Xuelan Zhang (SDUTCM: Shandong University of Traditional Chinese Medicine)H-Index: 2
view all 10 authors...
Ligustri Lucidi Fructus (LLF) is the dried and mature fruit of Ligubtrum lucidum Ait., which has the effect of nourishing the liver and kidney, brightening the eyes and promoting the growth of black hair. Wine-processed LLF is commonly used in traditional Chinese medicine; however, the processing mechanisms are still unclear. Herein, a system data acquisition and mining strategy was designed to investigate the chemical profile differences between the raw and wine-processed LLF, based on high-per...
#1Yumeng Zhang (SPU: Shenyang Pharmaceutical University)H-Index: 4
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Miao Wang (SPU: Shenyang Pharmaceutical University)H-Index: 9
view all 6 authors...
Abstract Yinchen Wuling Powder (YCWLP) is a classic Chinese medicine prescription with a long history and has been commonly used for treating jaundice hepatitis, liver fibrosis, hyperlipidemia and early diabetes in clinical applications. However, the chemical composition of YCWLP is still unclear. In order to obtain the chemical profile of YCWLP, a systematic ultra-performance liquid chromatography coupled with fourier transform ion cyclotron resonance mass spectrometry (UPLC-FT-ICR-MS) method w...
#1Qi Wang (JNU: Jinan University)H-Index: 1
#2Zhenyu Zou (JNU: Jinan University)
Last. Zhihong Yao (JNU: Jinan University)H-Index: 16
view all 9 authors...
Wendan decoction, a classical traditional Chinese medicine formula consisting of six herbal medicines, has been widely used in clinical treatments for thousands of years due to the expectorant effects. However, the chemical basis of Wendan decoction remains unclear, which hinders the elucidation of the scientific connotation and mechanism of its effective components. In this study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry met...
#1Qian Huang (CAS: Chinese Academy of Sciences)
#2Ji-Jun Chen (CAS: Chinese Academy of Sciences)H-Index: 28
Last. Chang-An Geng (CAS: Chinese Academy of Sciences)H-Index: 20
view all 7 authors...
Paeonia delavayi (Paeoniaceae), an endemic plant mainly distributed in southwest China, is always used as the substitute of P. suffruticosa due to their morphological and pharmacological similarity. In the previous study, P. suffruticosa was revealed with antidiabetic potency, whereas the chemical difference and antidiabetic property between different parts of P. delavayi has not yet been studied. This paper was designed to clarify the chemical constituents and antidiabetic potency of P. delavay...
#1Qiyuan Shan (ZJNU: Zhejiang Chinese Medical University)H-Index: 3
#1Qiyuan Shan (ZJNU: Zhejiang Chinese Medical University)
Last. Lu-Ping Qin (ZJNU: Zhejiang Chinese Medical University)H-Index: 52
view all 12 authors...
ABSTRACT Ethnopharmacological relevance The dried and nearly ripe fruits of Tetradium ruticarpum (A. Juss.) T.G. Hartley (TR) have long been used in treating headache and gastrointestinal disorders in oriental medicine. TR is usually processed by stir-frying with licorice extract before use. Although processing procedure is considered as the way to relieve pungent smell, reduce toxicity, and improve efficacy, its effects on TR’s toxicity and efficacy and bioactive compound profiles are largely u...
#1Zhenying Mei (SYSU: Sun Yat-sen University)H-Index: 1
#2Rongfei Zhang (SYSU: Sun Yat-sen University)H-Index: 2
Last. Guodong Zheng (Guangzhou Medical University)H-Index: 4
view all 7 authors...
Citrus reticulata cv. Chachiensis (CRC) has been widely used in many Chinese medicine prescriptions and developed as various health products. Due to its extensive biological activities, increased interest has focused on CRC as a potential source for the treatment of various diseases. However, there is no systematic and sufficient research on optimization and characterization of ultrasound extraction of antioxidant compounds from CRC up to now. In the present work, three parameters including ultr...
#1Qianqian Xue (Nanjing University of Chinese Medicine)
#2Yulin Wang (Nanjing University of Chinese Medicine)H-Index: 1
Last. Lin Li (Nanjing University of Chinese Medicine)H-Index: 7
view all 8 authors...
RATIONALE Crataegi Fructus (CF) is one of the most commonly used herbal medicines with a long history of clinical applications. CF is often processed to minimize gastric membrane irritation, although differently processed products can have different biological effects. The purpose of this study was to comprehensively identify the chemical composition of CF, determine the changes caused by processing, and elucidate the active constituents causing the clinical effects. This study aimed to define a...
#1Rui Mi (Anhui University of Chinese Medicine)H-Index: 1
#2Xiaofeng Li (Fudan University)H-Index: 1
Last. Xinrong Zhang ('SMMU': Second Military Medical University)H-Index: 2
view all 11 authors...
Discovering marker components of traditional Chinese medicine formulas is challenging because of the hundreds of components they inherently contain. This study first proposed a reliable and validated method for the comprehensive profiling of chemical constituents in Honghua Xiaoyao tablet by using high-performance liquid chromatography coupled with mass spectrometry. After searching within the in-house library, a total of 55 constituents were unambiguously characterized or tentatively identified...
2 CitationsSource
#1Xiaoman Mai (Guangdong Pharmaceutical University)H-Index: 3
#2Yingtao Liu (Guangdong Pharmaceutical University)H-Index: 3
Last. Pingfan LiH-Index: 2
view all 9 authors...
Abstract A green method for simultaneous extraction and enrichment of flavonoids from Euonymus alatus was developed by ultrasonic-assisted extraction (UAE) and temperature-induced cloud point extraction (TICPE) using PEG-base aqueous solution as extractant. Based on screening different molecular weights of PEGs, PEG-400/water was used as extractant, and the effects of key factors on extraction yields of flavonoids were investigated by single-factor experiment and response surface methodology (RS...
8 CitationsSource