Metabolic profiling of Gegenqinlian decoction in rat plasma, urine, bile and feces after oral administration by ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry.

Published on Mar 15, 2018in Journal of Chromatography B3.004
· DOI :10.1016/J.JCHROMB.2018.02.001
Ting Liu6
Estimated H-index: 6
(SPU: Shenyang Pharmaceutical University),
Xiumin Tian3
Estimated H-index: 3
(SPU: Shenyang Pharmaceutical University)
+ 4 AuthorsZhiguo Yu16
Estimated H-index: 16
(SPU: Shenyang Pharmaceutical University)
Abstract Gegenqinlian decoction (GQLD) as a traditional Chinese medical (TCM) prescription is made from four Chinese herbs and has been used for centuries to treat pyrexia and diarrhea. In this work, an ultra-high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was established to profile the metabolic fate of GQLD in rat plasma, urine, bile and feces. The analysis was performed on a Waters Acquity UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) at 35 °C within 25 min. A total of 174 compounds mainly including flavonoids, alkaloids and triterpenoids in rat plasma, urine, bile and feces after oral administration of GQLD were identified or tentatively characterized by comparison with retention times, accurate mass within 5 ppm error and their characteristic MS fragmentation ions. Among compounds in rats, 107 compounds are prototypes and 67 compounds are metabolites. Results demonstrated that metabolic pathways of GQLD in rats included methylation, demethylation, hydrogenation, hydrolysis, hydroxylation, methoxylation, sulfate conjugation and glucuronide conjugation. A clear understanding of the absorption and metabolism of GQLD is very important in its rational clinical use and pharmacological research. These findings enhance our understanding of the metabolism and effective forms of GQLD.
📖 Papers frequently viewed together
13 Citations
3 Citations
3 Citations
#1Xiao Ling (Southern Medical University)H-Index: 3
#2Yuqiang Xiang (Southern Medical University)H-Index: 3
Last. Xiaomei Tan (Southern Medical University)H-Index: 8
view all 5 authors...
Abstract Healthy animals are most widely used in current pharmacokinetic(PK) studies. However, neglecting the effects of specific diseases on drug absorption results in the PK parameters of those experiments not accurately reflecting in vivo drug concentration changes during treatment. In this study, an E. coli infective diarrheal minipig model was applied to explore the pharmacokinetics of Gegen Qinlian decoction (GQD). A simple and rapid ultrahigh performance liquid chromatography–tandem mass ...
13 CitationsSource
#1Fei Han (SPU: Shenyang Pharmaceutical University)H-Index: 17
#2Yanting Li (SPU: Shenyang Pharmaceutical University)H-Index: 6
Last. Ran Yin (SPU: Shenyang Pharmaceutical University)H-Index: 11
view all 8 authors...
Abstract A rapid and sensitive UHPLC-FT-ICR MS/MS method was developed for the first time to analyze the extract of Rhodiola crenulata and the constituents absorbed into rat blood and brain after oral administration. Under the optimized conditions, a total of 64 chemical constituents were identified or tentatively characterized in vitro in 30 min, and also 24 and 9 chemical constituents were detected in rat plasma and brain respectively, by comparing the retention time, accurate mass and/or MS/M...
26 CitationsSource
#1Zhou Wang (SDU: Shandong University)H-Index: 2
#2Jingui Yu (SDU: Shandong University)H-Index: 2
Last. Zhijie Xu (SDU: Shandong University)H-Index: 2
view all 7 authors...
Abstract Aims Ischemic heart disease is a leading cause of death and disability worldwide. Despite recent advances, there is no effective therapy for preventing myocardial ischemia–reperfusion (I/R) injury. In this study, we aimed to examine the therapeutic effect of scutellarin, a flavone isolated from the traditional Chinese medicine Scutellaria barbata and Erigeron breviscapus , on cardiomyocyte I/R injury. Main methods Neonatal rat cardiomyoblast cells H9C2 were used to study the role of scu...
64 CitationsSource
#1Qi Wang (PKU: Peking University)H-Index: 18
#2Wei Song (PKU: Peking University)H-Index: 16
Last. Min Ye (PKU: Peking University)H-Index: 46
view all 9 authors...
Abstract The quality evaluation of patent drugs derived from traditional Chinese medicine (TCM) compound formulas has been challenging due to their complex chemical composition. In this study, we developed a solution to evaluate the quality of Gegen-Qinlian Decoction (GQD), an ancient four-herb TCM formula for the treatment of diarrhea and diabetes, together with its derived patent drugs by simultaneously quantifying 50 bioactive compounds. The samples were extracted by 100% methanol (for hydrop...
43 CitationsSource
#1Shuai Ji (PKU: Peking University)H-Index: 19
#2Ru Li (PKU: Peking University)H-Index: 4
Last. Min Ye (PKU: Peking University)H-Index: 46
view all 10 authors...
Abstract Ethnopharmacological relevance Huang-Qin, derived from the roots of Scutellaria baicalensis Georgi, is a popular Chinese herbal medicine mainly used to treat influenza and cancer. This study aims to elucidate the anti-influenza, anti-cancer and anti-oxidation effective components of S. baicalensis . Materials and methods Various column chromatography techniques and semi-preparative HPLC were used to isolate Scutellaria compounds, and their structures were identified by HRESIMS and NMR s...
54 CitationsSource
#1Jiahao Huang (Guangxi Medical University)H-Index: 8
#2Mao Xie (Guangxi Medical University)H-Index: 1
Last. Weizhong Tang (Guangxi Medical University)H-Index: 1
view all 12 authors...
Abstract Formononetin (FT) is a natural phytoestrogen isolated from red clover ( Trifolium pratense L . ). Growing evidences indicate that FT-mediated cell arrest in cancers in vitro . However, detailed investigations on colorectal cancer by FT remain limited. In this study, we aimed to explore the potential benefits of FT and discuss the underlying mechanisms. FT-administered human colorectal cancer cells (RKO cell line) were subjected to testing the changes of endogenous expressions of gene/pr...
12 CitationsSource
#1Beilei Xu (Harbin University of Commerce)H-Index: 1
#2Pengyue LiH-Index: 1
Last. Guijun Zhang (Beijing University of Chinese Medicine)H-Index: 1
view all 3 authors...
Abstract An LC–MS/MS method was developed for simultaneous analysis of puerarin, daidzin, baicalin, glycyrrhizic acid, liquiritin, berberine, palmatine and jateorhizine of Gegenqinlian Decoction (GQD) and active components alignment (ACA) in rat plasma using hesperidin as the internal standard (I.S.). Chromatography was performed using a C18 column, with gradient elution with 1% acetic acid–0.001 mol/L ammonium acetate and acetonitrile at 0.2 ml/min. All analytes including I.S. were monitored un...
25 CitationsSource
#1Jia-Yu Zhang (Beijing University of Chinese Medicine)H-Index: 18
#2Wei Cai (Beijing University of Chinese Medicine)H-Index: 10
Last. Yanjiang Qiao (Beijing University of Chinese Medicine)H-Index: 7
view all 8 authors...
Abstract Baicalin (baicalein 7-O-glucuronide), which is one of the major bioactive constituents isolated from Scutellariae Radix, possesses many biological activities, such as antiallergic, antioxidation, and anti-inflammatory activities. In the present study, an efficient strategy was established using ultra-high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometer (UPLC-LTQ-Orbitrap MS) to profile the in vivo metabolic fate of baicalin in rat plasma, urine,...
52 CitationsSource
#1MaH-Index: 1
#2Shi-TangH-Index: 1
Last. Wen-ZhengH-Index: 1
view all 17 authors...
7 Citations
#1Shi-Tang Ma (Anhui University of Science and Technology)H-Index: 1
#2Cheng-Tao Feng (Anhui University of Science and Technology)H-Index: 12
Last. Wen-Zheng Ju (NU: Nanjing University)H-Index: 2
view all 9 authors...
The present study was designed to target fish for potential bioactive components contained in a Huang Lian Jie Du decoction (HLJDD) and identify the underlying mechanisms of action for the treatment of sepsis at the molecular level. he bioactive components database of HLJDD was constructed and the sepsis-associated targets were comprehensively investigated. The 3D structures of the PAFR and TXA2R proteins were established using the homology modelling (HM) method, and the molecular effects for se...
14 CitationsSource
Cited By17
#1Yan-Ping Li (Beijing University of Chinese Medicine)
#2Yi-Ting Gong (Beijing University of Chinese Medicine)H-Index: 2
Last. Ling Dong (Beijing University of Chinese Medicine)H-Index: 2
view all 9 authors...
ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian Decoction (GQD) (including: Puerariae lobatae (Willd.) Ohwi, radix; (short for Gengen) Glycyrrhiza uralensis Fisch., root and rhizome (short for Gancao), honeyed; Coptis chinensis Franch., rhizome (short for Huanglian); Scutellaria baicalensis Georgi, radix, boiled (short for S. baicalensis) has been widely used to treat inflammatory bowel disease (IBD) and colorectal cancer (CRC). To explore compatibility mechanism of GQD could be of advantage to inv...
#1Ting Liu (Shenyang Medical College)H-Index: 2
#1Ting Liu (SPU: Shenyang Pharmaceutical University)H-Index: 6
Last. Zhiguo Yu (SPU: Shenyang Pharmaceutical University)H-Index: 16
view all 6 authors...
An ultra-high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) metabonomic approach was employed to screen the differential components between normal rats and yeast-induced pyrexia rats after an oral administration of Gegenqinlian decoction (GQLD). Nine compounds, including puerarin, daidzein, baicalin, wogonoside, wogonin, berberine, palmatine, jateorhizine, and coptisine were identified as differential components in t...
Last. Bing-Liang Ma (SHU: Shanghai University)H-Index: 13
view all 3 authors...
Gegen-Qinlian decoction (GQD) is a classic traditional Chinese medicine (TCM) formula. It is composed of four TCMs, including Puerariae Lobatae Radix, Scutellariae Radix, Coptidis Rhizoma, and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle. GQD is traditionally and clinically used to treat both the "external and internal symptoms" of diarrhea with fever. In this review, key words related to GQD were searched in the Web of Science, PubMed, China National Knowledge Infrastructure (CNKI), and o...
#1Wei Wei (Xida: Guangxi University)
#2Siwei Li (Xida: Guangxi University)
Last. Xinsheng Yao (MOE: Chinese Ministry of Education)
view all 8 authors...
Background Yiganmingmu oral liquid (YGMM), a well known over-the-counter (OTC) drug in China, is composed of 12 types of valuable herbal medicines and has been widely used in clinical for the treatment of soreness and weakness of waist and knees, dizziness, memory loss, and fatigue. However, the chemical compositions of YGMM and its absorbed compounds in plasma remain unclear. Methods Since chemical investigation is the first important step to reveal effects and action mechanisms of traditional ...
#1Lin XuH-Index: 2
#2Jiaqi ZhangH-Index: 3
Last. Xu-Dong Tang (Peking Union Medical College)H-Index: 5
view all 6 authors...
BACKGROUND Ge-Gen-Qin-Lian Decoction (GGQLD), a traditional Chinese medicine (TCM) formula, has been widely used for ulcerative colitis (UC) in China, but the pharmacological mechanisms remain unclear. This research was designed to clarify the underlying pharmacological mechanism of GGQLD against UC. Method: In this research, a GGQLD-compound-target-UC network was constructed based on public databases to clarify the relationship between active compounds in GGQLD and potential targets. Gene ontol...
1 CitationsSource
#1Yuqi Ying (ZJNU: Zhejiang Chinese Medical University)
view all 6 authors...
Gegen Qinlian Decoction (GQD), a classic Chinese herbal formula, has been widely used in Chinese clinic for centuries and is well defined in treating nonalcoholic fatty liver disease (NAFLD). However, the mechanism action of GQD on NAFLD is still rarely evaluated. The present study aims to investigate the effect of GQD on treatment of NAFLD in rats and to further explore the underlying mechanism. The rat NAFLD model established by high-fat-diet feeding was used in the research. Our results exhib...
#1Xiu‐xiu Dou (SHU: Shanghai University)H-Index: 1
#2Shan LinH-Index: 6
Last. Weidong ZhangH-Index: 58
view all 7 authors...
Dingkun Dan (DKD), a famous traditional Chinese medicine prescription since Qing Dynasty (1739), has been widely used in the treatment of irregular menstruation, leucorrhea abnormality and postpartum gynecological disease. It is comprised by 30 various types of herbal and animal orient medicine, which results in the complexity of its chemical compositions. In this study, an integrative method was established to rapidly characterize the chemical components of DKD by using UPLC-Q-TOF/MSE combined ...
2 CitationsSource
#1Rongrong Li (Shanxi University)
#2Yanzhi LiuH-Index: 1
Last. Zhenyu Li (Shanxi University)H-Index: 25
view all 8 authors...
Naozhenning (NZN) granule, a Chinese herbal formula, is widely used to treat craniocerebral trauma and promote functional recovery. In our previous study, the chemical components, as well as the serum metabolites in the male Sprague-Dawley rats of the NZN granule after oral administration were characterized. In this study, the urine metabolites in the male Sprague-Dawley rats were further investigated by ultrahigh-performance liquid chromatography-Q Exactive hybrid quadrupole-Orbitrap high-resol...
#1Shu-Ling Zhao (CPU: China Pharmaceutical University)
#2Ping Li (CPU: China Pharmaceutical University)H-Index: 76
Last. Jie Yang (CPU: China Pharmaceutical University)H-Index: 16
view all 4 authors...
Abstract An improved bile microdialysis sampling technique was established and coupled with liquid chromatography quadrupole time-of-flight mass spectrometry (LC-qTOF-MS) analysis. This method was successfully applied to investigate the metabolic profiles of Ermiao wan (EMW) formula in the bile of Sprague-Dawley (SD) rats. Based on accurate mass information and fragment patterns, 23 alkaloids and lactones metabolites were tentatively identified. Their metabolic pathway involved in glucuronidatio...
#1Yanhui Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 1
#2Miao Wang (SPU: Shenyang Pharmaceutical University)H-Index: 9
Last. Chunjie Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 6
view all 6 authors...
Sanhua decoction, a famous Chinese herbal formula has been widely used for the treatment of stroke. In our study, a rapid, swift and straightforward analytical method with the help of UHPLC-FT-ICR-MS/MS was successfully developed for the first time to separate and identify the chemical constituents of Sanhua decoction. Chromatography was performed on a Universal XB C18 column (150 mm × 2.1 mm, 1.8 μm) using a mobile phase containing 0.1% formic acid–water (A) and acetonitrile (B). A total of 137...
1 CitationsSource