Recombinase

Biology

Gene

Recombination

Genotyping

Genotype

Computational biology

Genetics

Trends in Genetics

The Cre–lox recombination approach is commonly used to generate cell-specific gene inactivation (or activation). We have noticed that the breeding and genotyping sections of papers utilizing Cre–lox techniques are frequently incomplete. While seemingly straightforward, there are important considerations that need to be implemented in the breeding and genotyping methods to prevent the introduction of experimental confounds. Germline recombination and transient expression of Cre recombinase during development are some examples of the complications that can occur, and conventional genotyping methods may fail to identify these events.

Detecting and Avoiding Problems When Using the Cre–lox System