Gene

World Wide Web

Biology

Computer science

Computational biology

Bacteriophage

Genetics

Genome

Cloning (programming)

Recombination

Escherichia coli

Gateway (web page)

Programming language

In vitro recombination

clone (Java method)

Peptide sequence

Restriction enzyme

Molecular cloning

Cold Spring Harbor Protocols

The Gateway recombinatorial cloning system was developed for cloning multiple DNA fragments in parallel (e.g., in 96-well formats) in a standardized manner using the same enzymes. Gateway cloning is based on the highly specific integration and excision reactions of bacteriophage λ into and out of the Escherichia coli genome. Because the sites of recombination (“ att ” sites) are much longer (25–242 bp) than restriction sites, they are extremely unlikely to occur by chance in DNA fragments. Therefore, the same recombination enzyme can be used to robustly clone many different fragments of variable size in parallel reactions.

Gateway Recombinational Cloning