Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a truncated version of α-glucosidase from Aspergillus niger

A truncated version of α-glucosidase gene (AglA) from Aspergillus niger, named as AglA-t in this study, was expressed in Pichia pastoris, and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that AglA was most close to the α-glucosidase from Aureobasidium pullulans. In our research, we found that AglA-t could promote the formation of 2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G) under optimized conditions. The major...