Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant

This study aims to determine the optimal cryopreservation protocol for whole ovaries intended for preservation of fertility in women.We investigated the optimal cryopreservation procedure for whole ovaries in a bovine model. The following parameters were investigated to determine their effect on ovarian tissue viability: type of cryoprotectant, administration route of the cryoprotectant (perfusion and/or submersion), and the maximum tolerable...