The protective role of Helicobacter pylori neutrophil-activating protein in childhood asthma

Helicobacter pylori quantity and HP-NAP gene expression were evaluated in the faeces of healthy and asthmatic children. H. pylori DNAs and RNAs were isolated from the stool samples of 92 asthmatic children (AC; 3–8 years) and 88 healthy controls (HC). Quantitative PCR was used to determine the quantity of H. pylori and HP-NAP expression relative to the 16S rRNA (reference gene). Gene expression was analysed using the delta delta-Ct method. H....