Polydatin reduces Staphylococcus aureus lipoteichoic acid-induced injury by attenuating reactive oxygen species generation and TLR2-NFκB signalling.

Published on May 19, 2017in Journal of Cellular and Molecular Medicine4.486
· DOI :10.1111/JCMM.13194
Gan Zhao18
Estimated H-index: 18
(HAU: Huazhong Agricultural University),
Kangfeng Jiang18
Estimated H-index: 18
(HAU: Huazhong Agricultural University)
+ 3 AuthorsXiuli Peng3
Estimated H-index: 3
(HAU: Huazhong Agricultural University)
Sources
Abstract
Staphylococcus aureus (S. aureus) causes severe inflammation in various infectious diseases, leading to high mortality. The clinical application of antibiotics has gained a significant curative effect. However, it has led to the emergence of various resistant bacteria. Therefore, in this study, we investigated the protective effect of polydatin (PD), a traditional Chinese medicine extract, on S. aureus lipoteichoic acid (LTA)-induced injury in vitro and in vivo. First, a significant improvement in the pathological conditions of PD in vivo was observed, suggesting that PD had a certain protective effect on LTA-induced injury in a mouse model. To further explore the underlying mechanisms of this protective effect of PD, LTA-induced murine macrophages were used in this study. The results have shown that PD could reduce the NF-κB p65, and IκBα phosphorylation levels increased by LTA, resulting in a decrease in the transcription of pro-inflammatory factors, such as TNF-α, IL-1β and IL-6. However, LTA can not only activate NF-κB through the recognition of TLR2 but also increase the level of intracellular reactive oxygen species (ROS), thereby activating NF-κB signalling. We also detected high levels of ROS that activate caspases 9 and 3 to induce apoptosis. In addition, using a specific NF-κB inhibitor that could attenuate apoptosis, namely NF-κB p65, acted as a pro-apoptotic transcription factor in LTA-induced murine macrophages. However, PD could inhibit the generation of ROS and NF-κB p65 activation, suggesting that PD suppressed LTA-induced injury by attenuating ROS generation and TLR2-NFκB signalling.
📖 Papers frequently viewed together
101 Citations
5 Authors (Peter Storz, ..., Alex Toker)
79 Citations
2006
4 Authors (C. Bubici, ..., Guido Franzoso)
References61
Newest
#1Asie SadeghiH-Index: 8
Last. Reza Meshkani (Tehran University of Medical Sciences)H-Index: 23
view all 4 authors...
: Resveratrol has been shown to exert anti-inflammatory and anti-oxidant effects in a variety of cell types, however, its role in prevention of inflammatory responses mediated by palmitate in skeletal muscle cells remains unexplored. In the present study, we investigated the effects of resveratrol on palmitate-induced inflammation and elucidated the underlying mechanisms in skeletal muscle cells. The results showed that palmitate significantly enhanced TNF-α and IL-6 mRNA expression and protein ...
52 CitationsSource
#1Nazir M. Khan (NEOMED: Northeast Ohio Medical University)H-Index: 16
#2Abdul Haseeb (NEOMED: Northeast Ohio Medical University)H-Index: 15
Last. Tariq M. Haqqi (NEOMED: Northeast Ohio Medical University)H-Index: 52
view all 6 authors...
Abstract Osteoarthritis (OA), characterized by progressive destruction of articular cartilage, is the most common form of human arthritis. Here, we evaluated the potential chondroprotective and anti-inflammatory effects of Wogonin, a naturally occurring flavonoid, in IL-1β-stimulated human OA chondrocytes and cartilage explants. Wogonin completely suppressed the expression and production of inflammatory mediators including IL-6, COX-2, PGE2, iNOS and NO in IL-1β-stimulated OA chondrocytes. Furth...
88 CitationsSource
#1Rui-Jun Li (CPU: China Pharmaceutical University)H-Index: 10
#2Cai-Yun Gao (CPU: China Pharmaceutical University)H-Index: 5
Last. Ling-Yi Kong (CPU: China Pharmaceutical University)H-Index: 58
view all 6 authors...
Physalis minima has been traditionally used as a folk herbal medicine in China for the treatment of many inflammatory diseases. However, little is known about its anti-inflammatory constituents and associated molecular mechanisms. In our study, withaphysalin A (WA) and 2, 3-dihydro-withaphysalin C (WC), two major withanolide-type compounds, were obtained from the anti-inflammatory fraction of P. minima. Both WA and WC significantly inhibited the production of nitrite oxide (NO), prostaglandin E2...
23 CitationsSource
#1Kely Campos Navegantes (UFPA: Federal University of Pará)H-Index: 3
#2Rafaelli de Souza Gomes (UFPA: Federal University of Pará)H-Index: 5
Last. Marta Chagas Monteiro (UFPA: Federal University of Pará)H-Index: 22
view all 6 authors...
Macrophages and neutrophils are key components involved in the regulation of numerous chronic inflammatory diseases, infectious disorders, and especially certain autoimmune disease. However, little is known regarding the contribution of these cells to the pathogenesis of autoimmune disorders. Recent studies have aimed to clarify certain important factors affecting the immunogenicity of these cells, including the type and dose of antigen, the microenvironment of the cell-antigen encounter, and th...
117 CitationsSource
#1Kangfeng Jiang (HAU: Huazhong Agricultural University)H-Index: 18
#2Gan Zhao (HAU: Huazhong Agricultural University)H-Index: 18
Last. Xiuli Peng (HAU: Huazhong Agricultural University)H-Index: 3
view all 8 authors...
Recent studies show that Polydatin (PD) extracted from the roots of Polygonum cuspidatum Sieb, a widely used traditional Chinese remedies, possesses anti-inflammatory activity in several experimental models. In this study, we investigated the anti-inflammatory effects of PD on Staphylococcus aureus -induced mastitis in mice and elucidated the potential mechanisms. In mice with S aureus -induced mastitis, administration of PD (15, 30, 45 mg/kg, ip) or dexamethasone (Dex, 5 mg/kg, ip) significantl...
30 CitationsSource
#1Vanessa Mateus (University of Lisbon)H-Index: 7
#2João Rocha (University of Lisbon)H-Index: 109
Last. Rui Pinto (University of Lisbon)H-Index: 30
view all 6 authors...
Introduction : Chronic low back pain causes a high level of disability and is a public health problem that currently affects a large percentage of the working population in Europe. Fatigue, although subjective and complex, seems to be one of the main symptoms of patients with chronic low back pain. The prevalence and pathogenesis of fatigue are not completely understood, but it is well known that fatigue interferes decisively in the perception of wellness and quality of life. This study aims to ...
12 CitationsSource
: Reactive oxygen species (ROS) play a key role in the pathogenesis of atherosclerosis. The main mechanisms which are involved are low-density lipoprotein oxidative modification, inactivation of nitric oxide and modulation of redox-sensitive signaling pathways. ROS contribute to several aspects of atherosclerosis including endothelial cell dysfunction, monocyte/macrophage recruitment and activation, stimulation of inflammation, and inducing smooth muscle cell migration and proliferation. NADPH o...
33 CitationsSource
#1Samantha K. Wall (University of Bern)H-Index: 9
#2Olga Wellnitz (University of Bern)H-Index: 22
Last. Rupert M. Bruckmaier (University of Bern)H-Index: 61
view all 5 authors...
Abstract Bacterial mastitis causes pathogen-dependent changes of the blood-milk barrier, and these changes can influence the differential transfer of blood components to milk. It is well known that gram-negative pathogens such as Escherichia coli can cause a greater activation of the immune system and thus a more comprehensive transfer of blood components including IgG than gram-positive pathogens such as Staphylococcus aureus . Supraphysiological doses of oxytocin (OT) have been shown to increa...
19 CitationsSource
#1Rosanna Di Paola (UNIME: University of Messina)H-Index: 69
#2Roberta Fusco (UNIME: University of Messina)H-Index: 28
Last. Salvatore Cuzzocrea (SLU: Saint Louis University)H-Index: 116
view all 7 authors...
Endometriosis is a chronic, painful disease characterized by the presence of endometrial glands and stroma outside the uterine cavity. Palmitoylethanolamide (PEA), an endogenous fatty acid amide, has anti-inflammatory and neuroprotective effects. PEA lacks free radical scavenging activity, unlike polydatin (PLD), a natural precursor of resveratrol. The aim of this study was to investigate the effect of orally administered co-micronized PEA/polydatin (m(PEA/PLD)) in an autologous rat model of sur...
26 CitationsSource
#1Qiu-Hong Meng (Fourth Military Medical University)H-Index: 4
#2Hongbao Liu (Fourth Military Medical University)H-Index: 7
Last. Jian-Bo Wang (Fourth Military Medical University)H-Index: 3
view all 3 authors...
Abstract Polydatin, a glucoside of resveratrol, recently has been demonstrated possibly to exert its biological effects by targeting sonic hedgehog (Shh). However, whether Shh signaling pathway is involved in the therapeutic effects of polydatin for renal ischemia/reperfusion (I/R) injury has not been evaluated. Our results showed that I/R induced the secretion of Shh, upregulated Patched and Smoothened, and enhanced the nuclear translocation and target gene transcription of Glioblastoma 1 in re...
29 CitationsSource
Cited By33
Newest
#1Aftab Shaukat (HAU: Huazhong Agricultural University)H-Index: 13
#2Irfan Shaukat (University of Lorraine)H-Index: 4
Last. Ganzhen Deng (HAU: Huazhong Agricultural University)H-Index: 20
view all 16 authors...
Acute lung injury (ALI) is acute uncontrolled inflammation of lung tissue that leads to high fatality both in human and animals. Staphylococcus aureus (S. aureus) could be an opportunistic, versatile bacterial etiology of ALI. Ginsenoside Rb1 (Rb1) is extracted from the Panax ginseng, which displays a wide range of biological and pharmacological effects. However, protective effects of Rb1 in S. aureus-induced ALI though endoplasmic reticulum (ER) stress and death receptor-mediated pathways have ...
Source
#1Aftab Shaukat (HAU: Huazhong Agricultural University)H-Index: 13
#2Sana Hanif (UOG: University of Gujrat)H-Index: 1
Last. Ganzhen Deng (HAU: Huazhong Agricultural University)H-Index: 20
view all 14 authors...
Abstract Pneumonia is the acute inflammation of lung tissue and is multi-factorial in etiology. Staphylococcus aureus (S. aureus) is a harmful pathogen present as a normal flora of skin and nares of dairy cattle. In bovine pneumonia, S. aureus triggers to activates Toll-Like Receptors (TLRs), that further elicits the activation of the inflammation via NF-κB pathway, oxidative stress and apoptotic pathways. In the current study, pathogen-associated gene expression of the pro-inflammatory cytokine...
Source
#1Talha Umar (HAU: Huazhong Agricultural University)H-Index: 5
#2Baoyi Yin (HAU: Huazhong Agricultural University)
Last. Ganzhen Deng (HAU: Huazhong Agricultural University)H-Index: 20
view all 9 authors...
Endometritis in dairy cows is a major economic problem worldwide; without advances in lifestyle management and drug treatment, it causes high morbidity and death. Micro ribonucleic acid (miRNAs) these days is seen as an important part of gene control networks. It is a class of small nucleotides 20-25, single-stranded RNA molecules. In endometritis, the inflammatory response caused by the gram-negative bacteria Escherichia coli (E. coli) alters the expression of miRNA which can regulate the innat...
Source
#2Jie LuoH-Index: 2
Last. Nan ZengH-Index: 2
view all 7 authors...
Numerous studies have revealed that oxidative stress is closely associated with the occurrence and development of depression. Xiaoyao Pills (XYW) are included in the Chinese Pharmacopoeia and are frequently used for treating anxiety and depression by smoothing the liver, strengthening the spleen, and nourishing the blood. However, the antidepressant effects of XYW have not yet been thoroughly investigated. The objective of our study was to investigate the antidepressant-like effects of XYW and t...
Source
#1Kangfeng Jiang (Yunnan Agricultural University)
#2Jing Yang (HAU: Huazhong Agricultural University)H-Index: 10
Last. Xiaobing Li (Yunnan Agricultural University)
view all 6 authors...
Endometritis is a reproductive disorder characterized by an inflammatory response in the endometrium, which causes significant economic losses to the dairy farming industry. MicroRNAs (miRNAs) are implicated in the inflammatory response and immune regulation following infection by pathogenic bacteria. Recent miRNA microarray analysis showed an altered expression of miR-92b in cows with endometritis. In the present study, we set out to investigate the regulatory mechanism of miR-92b in endometrit...
Source
#1Tao ZhangH-Index: 12
#2Xiujuan FengH-Index: 1
Last. Changwei Qiu (HAU: Huazhong Agricultural University)H-Index: 18
view all 9 authors...
Since companion dogs have the same living environment as humans, they are a good animal model for the study of human diseases; this is especially true of canine spontaneous mammary tumors models. A better understanding of the natural history and molecular mechanisms of canine mammary tumor is of great significance in comparative medicine. Here, we collected canine mammary tumor cases and then assayed the clinical cases by pathological examination and classification by HE staining and IHC. miRNA-...
1 CitationsSource
#1Yurong Fu (JLU: Jilin University)H-Index: 4
#2Yongcheng Jin (JLU: Jilin University)H-Index: 8
Last. Yue Tian (JLU: Jilin University)
view all 12 authors...
Zearalenone (ZEA) is a mycotoxin of the Fusarium genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from Polygonum cuspidatum, has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 μM) gradually decreased the cell viability. ...
Source
Background Gastric cancer is the second leading cancer-related mortality worldwide and more effective treatment strategies are urgently needed to combat the disease. Using lipoteichoic acid (LTA) and miR-27b-3p agomir, we aimed to assess the efficacy of this combination of therapies in treating gastric cancer. Methods The RNA levels of miR-27b-3p, FOXO3, MET, KRAS, vascular endothelial growth factor C (VEGFC), TSC1, and P65 were analyzed by quantified-PCR (Q-PCR) and the cell viability of AGS ce...
Source
#1Zhihao Hao (SDU: Shandong University)
#3Lulin Rao (WMU: Wenzhou Medical College)
Source
#1Mengyun Zou (HAU: Huazhong Agricultural University)H-Index: 4
#2Wenlong Yang (HAU: Huazhong Agricultural University)H-Index: 1
Last. Xiuli Peng (HAU: Huazhong Agricultural University)H-Index: 12
view all 7 authors...
Abstract Mycoplasma gallisepticum (MG) infection is the main cause of chronic respiratory disease (CRD) characterized by severe respiratory inflammation in chickens. Polydatin (PD) is a resveratrol glycoside isolated from Polygonum cuspidatum, which has prominent anti-inflammatory effect. The purpose of this study was to investigate the therapeutic effect of PD against MG-induced inflammation in chicken and its underlying mechanism. Histopathological analysis showed that PD treatment (15, 30, an...
4 CitationsSource