Identification by highly sensitive 16S metagenomic sequencing of an unusual case of polymicrobial bacteremia

•Current culture-based diagnosis of BSI is limited by frequency of false negatives. •Polymicrobial infections, which are more severe, are extremely difficult to detect. •We have developed molecular assays capable of detecting BSI with high sensitivity. •We detected an unusual BSI involving Staphylococcus, Microbacterium and Janibacter •This study provides insights into polymicrobial BSI associated with heart...