Glycated Collagen Stimulates Differentiation of Gingival Myofibroblasts

Published on Sep 1, 2017in Journal of Periodontology3.742
· DOI :10.1902/JOP.2017.160730
Ignacio N. Retamal6
Estimated H-index: 6
(UC: Pontifical Catholic University of Chile),
Romina Hernández3
Estimated H-index: 3
(UC: Pontifical Catholic University of Chile)
+ 4 AuthorsPatricio C. Smith22
Estimated H-index: 22
(UC: Pontifical Catholic University of Chile)
Background: Glucose-derived metabolites may alter the structure and biologic properties of important proteins in periodontium, such as collagens. As a consequence, it is possible that collagen-binding cells may change their phenotypic traits. Although the glucose-derived product methylglyoxal (MGO) has been detected in periodontal lesions, the precise effect of collagen glycation on gingival connective tissue biology is not fully understood. The present study evaluates whether collagen glycation by MGO may affect phenotypic properties and remodeling capacity of human gingival fibroblasts (HGFs).Methods: Primary cultures of HGFs were grown on Type I collagen matrices previously treated with MGO. Cell cultures were tested for cell viability, apoptosis, α-smooth muscle actin (SMA), fibronectin (FN) production, and collagen remodeling. Mechanical properties and morphology of MGO-treated collagen gels were evaluated using rheometry and atomic force microscopy. Statistical analysis was performed by Kruskal-Wall...
📖 Papers frequently viewed together
49 Citations
94 Citations
#1Ignacio N. Retamal (UC: Pontifical Catholic University of Chile)H-Index: 6
#2Ramón Faustino Díaz Hernández (UC: Pontifical Catholic University of Chile)H-Index: 3
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 10 authors...
Background and Objectives Methylglyoxal is a toxic product derived from glucose metabolism that plays a role in inflammation, diabetes and aging. In addition, the periodontal pathogen Tannerella forsythensis may also generate this compound. However, the effects of methylglyoxal on gingival cells are still poorly understood. In the present study, we have explored whether methylglyoxal or methylglyoxal-treated collagen may modulate cell viability, death and proliferation in gingival connective tis...
5 CitationsSource
#1Nuno M. Coelho (U of T: University of Toronto)H-Index: 10
#2Christopher A. McCulloch (U of T: University of Toronto)H-Index: 86
Fibrosis is the result of a wound-healing response that fails to restore normal tissue structure function. One of the critical hallmarks of fibrosis is disrupted collagen remodeling. In tissue homeostasis, the production, deposition and organization of collagen is balanced by the degradation and remodeling of collagen within the existing matrix. After injury or chronic infection, tissues initiate a wound-healing response that is intended to create a new ECM for restoring tissue structure and fun...
37 CitationsSource
#1Akira Onishi (Jichi Medical University)H-Index: 10
#2Tetsu Akimoto (Jichi Medical University)H-Index: 20
Last. Eiji Kusano (Jichi Medical University)H-Index: 4
view all 8 authors...
Peritoneal fibrosis (PF), a serious pathophysiology of peritoneal dialysis (PD), is implicated in various types of chronic inflammation. In the present study, we examined the benefits of interleukin (IL)-10, which exerts anti-inflammatory effects, in an experimental rat model of methylglyoxal (MGO)-induced PF. We injected an adeno-associated virus (AAV) vector encoding rat IL-10 or enhanced green fluorescent protein (GFP) into male Sprague-Dawley rats at 6 weeks of age. Four weeks later, the rat...
10 CitationsSource
#1Katarzyna I. Wolanska (University of Liverpool)H-Index: 2
#2Mark R. Morgan (University of Liverpool)H-Index: 17
The biophysical, mechanical and chemical characteristics of extracellular matrixes influence many cellular functions to control tissue homoeostasis and drive progression of cancer and inflammatory diseases. To maintain normal tissue function, fibronectin-rich matrixes are subject to dynamic cell-mediated structural and chemical modification. In this article, we discuss how localized application of mechanical force, heterodimer-specific integrin engagement and matrix proteolysis regulate fibronec...
12 CitationsSource
#1Ilana Talior-Volodarsky (U of T: University of Toronto)H-Index: 8
#2Pamma D. Arora (U of T: University of Toronto)H-Index: 15
Last. Christopher A. McCulloch (U of T: University of Toronto)H-Index: 86
view all 7 authors...
The adhesion of cardiac fibroblasts to the glycated collagen interstitium in diabetics is associated with de novo expression of the α11 integrin, myofibroblast formation and cardiac fibrosis. We examined how methylglyoxal-glycated collagen regulates α11 integrin expression. In cardiac fibroblasts plated on glycated collagen but not glycated fibronectin, there was markedly increased α11 integrin and α-smooth muscle actin expression. Compared with native collagen, binding of purified α11β1 integri...
26 CitationsSource
#1Saul Genuth (Case Western Reserve University)H-Index: 59
#2Wanjie Sun (GW: George Washington University)H-Index: 23
Last. Vincent M. MonnierH-Index: 79
view all 7 authors...
Six skin collagen AGEs originally measured near Diabetes Control and Complications Trial (DCCT) closeout in 1993 may contribute to the “metabolic memory” phenomenon reported in the follow-up EDIC complications study. We now investigated whether addition of 4 originally unavailable AGEs, i.e. glucosepane (GSPNE), hydroimidazolones of methylglyoxal (MG-H1) and glyoxal (G-H1), and carboxyethyl-lysine (CEL), improves associations with incident retinopathy , nephropathy , and neuropathy events during...
84 CitationsSource
#1Mónica Cáceres (University of Chile)H-Index: 16
#2Alejandro OyarzúnH-Index: 9
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 3 authors...
Aging may negatively affect gingival wound-healing. However, little is known about the mechanisms underlying this phenomenon. The present study examined the cellular responses associated with gingival wound-healing in aging. Primary cultures of human gingival fibroblasts were obtained from healthy young and aged donors for the analysis of cell proliferation, cell invasion, myofibroblastic differentiation, and collagen gel remodeling. Serum from young and old rats was used to stimulate cell migra...
42 CitationsSource
#1Cara Lea Carraher (Princeton University)H-Index: 4
#2Jean E. Schwarzbauer (Princeton University)H-Index: 65
Cells sense and respond to the mechanical properties of their microenvironment. We investigated whether these properties affect the ability of cells to assemble a fibrillar fibronectin (FN) matrix. Analysis of matrix assembled by cells grown on FN-coated polyacrylamide gels of varying stiffnesses showed that rigid substrates stimulate FN matrix assembly and activation of focal adhesion kinase (FAK) compared with the level of assembly and FAK signaling on softer substrates. Stimulating integrins ...
34 CitationsSource
#1Franco Klingberg (U of T: University of Toronto)H-Index: 4
#2Boris Hinz (U of T: University of Toronto)H-Index: 69
Last. Eric S. White (UM: University of Michigan)H-Index: 68
view all 3 authors...
Myofibroblasts, and the extracellular matrix (ECM) in which they reside, are critical components of wound healing and fibrosis. The ECM, traditionally viewed as the structural elements within which cells reside, is actually a functional tissue whose components possess not only scaffolding characteristics, but also growth factor, mitogenic, and other bioactive properties. Although it has been suggested that tissue fibrosis simply reflects an ‘exuberant’ wound-healing response, examination of the ...
376 CitationsSource
#1Thomas Fleming (Heidelberg University)H-Index: 27
#2Till Martin Theilen (Heidelberg University)H-Index: 1
Last. Peter P. Nawroth (Heidelberg University)H-Index: 115
view all 16 authors...
Methylglyoxal (MG), the major dicarbonyl substrate of the enzyme glyoxalase 1 (GLO1), is a reactive metabolite formed via glycolytic flux. Decreased GLO1 activity in situ has been shown to result in an accumulation of MG and increased formation of advanced glycation endproducts, both of which can accumulate during physiological aging and at an accelerated rate in diabetes and other chronic degenerative diseases. To determine the physiological consequences which result from elevated MG levels and...
43 CitationsSource
Cited By4
#1Chia En Lin (TMU: Taipei Medical University)H-Index: 3
#1Chia-En Lin (TMU: Taipei Medical University)
Last. S.-M. Chen (TMU: Taipei Medical University)H-Index: 1
view all 9 authors...
ETHNOPHARMACOLOGICAL RELEVANCE Most Aristolochiaceae plants are prohibited due to aristolochic acid nephropathy (AAN), except Xixin (Asarum spp.). Xixin contains trace amounts of aristolochic acid (AA) and is widely used in Traditional Chinese Medicine. Methylglyoxal and D-lactate are regarded as biomarkers for nephrotoxicity. AIM OF THE STUDY The use of Xixin (Asarum spp.) is essential and controversial. This study aimed to evaluate tubulointerstitial injury and interstitial renal fibrosis by d...
#1Ignacio N. Retamal (University of Los Andes)H-Index: 6
#1Ignacio N Retamal (University of Los Andes)H-Index: 1
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 8 authors...
OBJECTIVES: Myofibroblasts constitute a specific cell phenotype involved in connective tissue healing. Diabetes alters the wound healing response. However, it is not clear whether diabetes modifies the involvement of myofibroblasts in periodontal wounds. MATERIAL AND METHODS: Type I diabetes was induced in rats through streptozotocin injection and periodontal wounds were performed. Wound healing was evaluated histologically at 2, 5, 7 and 15 days by measuring epithelial migration, neutrophil inf...
3 CitationsSource
AbstractBackground: This study explored the function and mechanism of miR-486-5p in HSFBs.Methods: Qualitative real-time-polymerase chain reaction (qRT-PCR) was performed to detect the expression o...
5 CitationsSource
#1Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
#2Constanza Martínez (UC: Pontifical Catholic University of Chile)H-Index: 12
Last. Christopher A. McCulloch (U of T: University of Toronto)H-Index: 86
view all 4 authors...
After injury to periodontal tissues a healing response is initiated that includes a series of sequential phases, which enable wound closure and partial restoration of tissue structure and function. Wound closure in periodontal tissues involves the tightly regulated coordination of resident cells in epithelial and connective tissue compartments. Multiple cell populations in these compartments synergize their metabolic activities to re-establish a mucosal seal that involves the underlying periodon...
35 CitationsSource