Glycated Collagen Stimulates Differentiation of Gingival Myofibroblasts

Published on Sep 1, 2017in Journal of Periodontology3.742
· DOI :10.1902/JOP.2017.160730
Ignacio N. Retamal6
Estimated H-index: 6
(UC: Pontifical Catholic University of Chile),
Romina Hernández3
Estimated H-index: 3
(UC: Pontifical Catholic University of Chile)
+ 4 AuthorsPatricio C. Smith22
Estimated H-index: 22
(UC: Pontifical Catholic University of Chile)
Sources
Abstract
Background: Glucose-derived metabolites may alter the structure and biologic properties of important proteins in periodontium, such as collagens. As a consequence, it is possible that collagen-binding cells may change their phenotypic traits. Although the glucose-derived product methylglyoxal (MGO) has been detected in periodontal lesions, the precise effect of collagen glycation on gingival connective tissue biology is not fully understood. The present study evaluates whether collagen glycation by MGO may affect phenotypic properties and remodeling capacity of human gingival fibroblasts (HGFs).Methods: Primary cultures of HGFs were grown on Type I collagen matrices previously treated with MGO. Cell cultures were tested for cell viability, apoptosis, α-smooth muscle actin (SMA), fibronectin (FN) production, and collagen remodeling. Mechanical properties and morphology of MGO-treated collagen gels were evaluated using rheometry and atomic force microscopy. Statistical analysis was performed by Kruskal-Wall...
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