Character and laxative activity of polysaccharides isolated from Dendrobium officinale

Dendrobium officinale was separated into total polysaccharides (DOP) and supernatant (DOS) to investigate the laxative ingredients and mechanism in normal and constipated mice induced by diphenoxylate or water fasting. The molecular weight and components of DOP were determined by HPGPC, HPLC and LC-MS. DOP was mainly composed of d-mannose (59.19 mg/g) and d-glucose (830.98 mg/g), with a molecular weight of 8404 Da. DOP (29, 57, 114 mg/kg, i.g.)...