RNA‐guided Cas9 as an in vivo desired‐target mutator in maize
Abstract
Summary The RNA ‐guided Cas9 system is a versatile tool for genome editing. Here, we established a RNA ‐guided endonuclease ( RGEN ) system as an in vivo desired‐target mutator ( DTM ) in maize to reduce the linkage drag during breeding procedure, using the LIGULELESS 1 ( LG 1 ) locus as a proof‐of‐concept. Our system showed 51.5%–91.2% mutation frequency in T0 transgenic plants. We then crossed the T1 plants stably expressing DTM with six...
Paper Details
Title
RNA‐guided Cas9 as an in vivo desired‐target mutator in maize
Published Date
Apr 5, 2017
Journal
Volume
15
Issue
12
Pages
1566 - 1576
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