Isolation and cultivation of adult primary bovine hepatocytes from abattoir derived liver.
Abstract
The aim was to establish a cell culture of adult primary bovine hepatocytes obtained from liver following slaughter and to cultivate the cells in a sandwich culture. Cells and medium samples were taken after separation of cells (day 0), during monolayer (days 1, 2 and 3) and during sandwich culture (days 1, 2, 3, 4, 7, 10 and 14). The mRNA expression of BAX, BCL2L, FAS, IGF-1 and GHR1A was measured as well as urea and LDH. Hepatocytes were...
Paper Details
Title
Isolation and cultivation of adult primary bovine hepatocytes from abattoir derived liver.
Published Date
Jan 1, 2016
Journal
Volume
15
Pages
858 - 866
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Notes
History