Antibodies to biotinylated red blood cells in adults and infants: improved detection, partial characterization, and dependence on red blood cell-biotin dose

BACKGROUND Biotin‐labeled red blood cells (BioRBCs) are used for in vivo kinetic studies. Because BioRBC dosing occasionally induces antibodies, a sensitive and specific anti‐BioRBC detection assay is needed. STUDY DESIGN AND METHODS Aims were to 1) develop a gel card assay to evaluate existing, naturally occurring and BioRBC‐induced plasma antibodies, 2) compare gel card and tube agglutination detection results, and 3) test for a relationship...