Rigid Spin Labels for Improved Distance and Dynamics in Intrinsically Disordered Proteins and Peptides

Spin-labeling and Electron Paramagnetic Resonance spectroscopy (EPR) have become powerful tools for structure determination in proteins and biomacromolecules. Usual spin labels, such as the disulfide-linked MTSL labels are flexible, owing to multiple single bonds separating the nitroxide from the protein backbone. We investigate spin labels rigidly linked to the backbone in a series of peptides derived from the KVKVLGDVIEV peptide[1], with the...