Efficacy of Korean Multipurpose Contact Lens Disinfecting Solutions against Acanthamoeba castellanii.

Published on Dec 31, 2016in Korean Journal of Parasitology1.311
· DOI :10.3347/KJP.2016.54.6.697
Eun-Kyung Moon8
Estimated H-index: 8
(Kyung Hee University),
Hye-Ryun Park1
Estimated H-index: 1
(Dong-a University)
+ 1 AuthorsHyun-Hee Kong23
Estimated H-index: 23
(Dong-a University)
: Acanthamoeba keratitis has been increasing in recent years. Main risk factors are contact lens wear and their cleaning solutions. Most contact lens wearers use multipurpose disinfecting solutions (MPDS) for cleansing and disinfecting microorganisms because of its convenience. We determined amoebicidal effects of MPDS made in Korea and their cytotoxicity on human corneal epithelium cells. Fifteen commercial MPDS (A to O) were tested for their amoebicidal effects on Acanthamoeba castellanii trophozoites and cysts by using a most probable number (MPN) technique. Among them, 7 kinds of MPDS showed little or no amoebicidal effects for 24 hr exposure. Solutions A, B, G, H, L, and O showed positive amoebicidal effects, and solutions M and N killed almost all trophozoites and cysts after 24 hr exposure. However, 50%-N solution showed 56% cytotoxicity on human corneal epithelial cells within 4 hr exposure, and 50%-O solution also showed 62% cytotoxicity on human cells within 4 hr exposure. Solution A did not show any cytotoxicity on human cells. These results revealed that most MPDS made in Korea were ineffective to kill Acanthamoeba. The solutions having amoebicidal activity also showed high levels of cytotoxicity on human corneal epithelial cells. New formulations for improved MPDS that are amoebicidal but safe for host cells are needed to prevent Acanthamoeba keratitis.
📖 Papers frequently viewed together
21 Citations
13 Citations
3 Citations
#1Eun-Kyung Moon (KNU: Kyungpook National University)H-Index: 12
#2Yeonchul HongH-Index: 19
Last. Hyun-Hee KongH-Index: 23
view all 5 authors...
The aim of this study was to improve the cytopathic effect (CPE) of antiamebic agents by combining with cellulose synthesis inhibitor as an encystation inhibitor.Cellulose synthesis inhibitors, 2,6-dichlorobenzonitrile (DCB) and isoxaben were used to block encystation of Acanthamoeba during cultivation. Cultured human corneal epithelial (HCE) cells and Acanthamoeba were treated with polyhexamethylene biguanide (PHMB) combined with cellulose synthesis inhibitors to evaluate the CPE as an antiameb...
16 CitationsSource
#1Eun-Kyung Moon (KNU: Kyungpook National University)H-Index: 12
#2So-Hee Kim (Dong-a University)H-Index: 2
Last. Hyun-Hee Kong (Dong-a University)H-Index: 23
view all 6 authors...
ABSTRACT Acanthamoeba cysts are resistant to extreme physical and chemical conditions. Autophagy is an essential pathway for encystation of Acanthamoeba cells. To evaluate the possibility of an autophagic Acanthamoeba encystation mechanism, we evaluated autophagy inhibitors, such as 3-methyladenine (3MA), LY294002, wortmannin, bafilomycin A, and chloroquine. Among these autophagy inhibitors, the use of 3MA and chloroquine showed a significant reduction in the encystation ratio in Acanthamoeba ce...
25 CitationsSource
#1Yousuf Aqeel (Aga Khan University)H-Index: 8
#2Ruqaiyyah Siddiqui (Aga Khan University)H-Index: 24
Last. Naveed Ahmed Khan (Aga Khan University)H-Index: 45
view all 3 authors...
A key challenge in the successful treatment of Acanthamoeba infections is its ability to transform into a dormant cyst form that is resistant to physiological conditions and pharmacological therapies, resulting in recurrent infections. The carbohydrate linkage analysis of cyst walls of Acanthamoeba castellanii showed variously linked sugar residues, including xylofuranose/xylopyranose, glucopyranose, mannopyranose, and galactopyranose. Here, it is shown that exogenous xylose significantly reduce...
19 CitationsSource
#1Camus Kar Man ChoyH-Index: 12
#2Pauline ChoH-Index: 37
Last. Maureen V. Boost (PolyU: Hong Kong Polytechnic University)H-Index: 23
view all 3 authors...
Purpose: The aim was to determine the cytotoxic effects of three multipurpose solutions (MPS) on human corneal epithelial cells (HCEC) and to assess the metabolic rates of recovering cells at different levels of cell membrane damage. Method: The effects of one to 15 minutes exposure to multipurpose solutions containing polyquaternium-1 (MPS-A), polyaminopropyl biguanide (MPS-B) and polyhexanide (MPS-C) on HCEC were determined. Recovery rates at different levels of cell membrane damage were asses...
29 CitationsSource
Objectives: To assess and compare the cytotoxicity and antimicrobial activity of six multipurpose disinfecting contact lens solutions (MPDS). Method: Six MPDS were assessed and compared: MeniCare Soft, Complete Moisture Plus, Solo-Care Aqua, Opti-Free Express, ReNu MultiPlus and ReNuMoistureLoc. Cytotoxicity was evaluated by means of the colony-forming assay (ISO 10993-5) using one cell line. One hundred Chinese hamster's fibroblast V79 cells in 2 mL of medium were inoculated into 6-well plates ...
101 CitationsSource
Abstract Purpose To evaluate the safety and efficacy of a new multi-purpose disinfecting solution (MPDS) in symptomatic soft contact lens wearers using Group IV soft lenses and compare its performance with that of a marketed MPS. Methods This was a 4-week, randomized, concurrently controlled, double-masked, multi-site study involving 362 subjects at 19 investigational sites in the US. Existing Group IV lens wearers, pre-screened to be symptomatic for contact lens-related ocular discomfort, were ...
51 CitationsSource
#1Ryoji Yanai (Yamaguchi University)H-Index: 24
#2Naoyuki Yamada (Yamaguchi University)H-Index: 17
Last. Teruo Nishida (Yamaguchi University)H-Index: 62
view all 9 authors...
Povidone-iodine (PVP-I) possesses broad-spectrum antimicrobial activity and is used clinically as a disinfectant. We evaluated the disinfectant properties and safety of PVP-I for use as a contact lens solution. The concentrations of PVP-I required to reduce the number of Staphylococcus aureus or Candida albicans by 3 log units were lower than were those of hydrogen peroxide, polyhexamethylene biguanide (PHMB), and benzalkonium chloride (BAK). The cytotoxicity of PVP-I for cultured human corneal ...
26 CitationsSource
#1Tara K. Beattie (Vision-Sciences, Inc.)H-Index: 15
#2David V. Seal (City University London)H-Index: 25
Last. Anthony Grimason (University of Strathclyde)H-Index: 10
view all 5 authors...
Six multipurpose contact lens solutions [All-in-One, All-in-One (Light), ReNu MultiPlus, Optifree Express, Complete, and Solo-care soft] were tested for their efficacies against Acanthamoeba castellanii trophozoites and cysts by using a most probable number (MPN) technique for amoebic enumeration. Against trophozoites, All-in-One, ReNu Multiplus, and Optifree Express achieved total kill (log reduction of >3) after the manufacturer's minimum recommended disinfection time (MMRDT), with the remaini...
84 CitationsSource
#1Francine Marciano-Cabral (VCU: Virginia Commonwealth University)H-Index: 27
#2Guy A. Cabral (VCU: Virginia Commonwealth University)H-Index: 35
Acanthamoeba spp. are free-living amebae that inhabit a variety of air, soil, and water environments. However, these amebae can also act as opportunistic as well as nonopportunistic pathogens. They are the causative agents of granulomatous amebic encephalitis and amebic keratitis and have been associated with cutaneous lesions and sinusitis. Immuno compromised individuals, including AIDS patients, are particularly susceptible to infections with Acanthamoeba. The immune defense mechanisms that op...
1,389 CitationsSource
#1K. Hiti (University of Graz)H-Index: 3
#2Julia WalochnikH-Index: 44
Last. Horst AspöckH-Index: 38
view all 5 authors...
Background/aim: Contact lens cases contaminated with Acanthamoeba are a major risk factor for an infection of the eye. In this study the anti-Acanthamoeba activity of three different contact lens storage solutions was tested. Methods: A new multipurpose contact lens storage solution (Meni Care Plus) and a two step (Titmus H2O2) and one step (Oxysept Comfort) hydrogen peroxide system were tested for their effects on trophozoites and cysts of three different Acanthamoeba species: A castellanii, A ...
131 CitationsSource
Cited By12
#1T. Martín-PérezH-Index: 4
Last. Jorge Pérez-SerranoH-Index: 16
view all 4 authors...
Ameobae belonging to the genus Acanthamoeba are responsible for the human diseases Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). The treatment of these illnesses is hampered by the existence of a resistance stage (cysts). In an attempt to add new agents that are effective against trophozoites and cysts, tea tree oil (TTO) and dimethyl sulfoxide (DMSO), separately and in combination, were tested In Vitro against two Acanthamoeba isolates, T3 and T4 genotypes. The oxyge...
#1Mark D. P. Willcox (UNSW: University of New South Wales)H-Index: 80
#2Nancy KeirH-Index: 12
Last. Lyndon Jones (UW: University of Waterloo)H-Index: 69
view all 11 authors...
Contact lens materials have undergone significant changes over the past 20 years, particularly with respect to the introduction of silicone hydrogel materials. Whilst this development addressed hypoxic issues, other important areas relating to contact lens success, notably comfort, require further research. Contact lens wettability remains a crucially important part of biocompatibility. Contact lenses can be made more wettable by incorporation of surfactants into blister packs, internal wetting ...
6 CitationsSource
#2Tommy Tong (Sunway University)H-Index: 12
Last. Ruqaiyyah SiddiquiH-Index: 24
view all 6 authors...
Acanthamoeba keratitis is a sight-endangering eye infection, and causative organism Acanthamoeba presents a significant concern to public health, given escalation of contact lens wearers. Contemporary therapy is burdensome, necessitating prompt diagnosis and aggressive treatment. None of the contact lens disinfectants (local and international) can eradicate Acanthamoeba effectively. Using a range of compounds targeting cellulose, ion channels, and biochemical pathways, we employed bioassay-guide...
#1Rosnani Hanim Mohd Hussain (UiTM: Universiti Teknologi MARA)H-Index: 1
#2Wan Nur Afiqah (UiTM: Universiti Teknologi MARA)
Last. Tengku Shahrul Anuar (UiTM: Universiti Teknologi MARA)H-Index: 13
view all 6 authors...
Abstract The incidence of Acanthamoeba keratitis has been increasing since the previous decades, especially among contact lens users. This infection is majorly caused by the use of ineffective contact lens disinfecting solution. Thus, this study was conducted to evaluate the in vitro effects of multi-purpose disinfecting solutions (MPDS) against Acanthamoeba trophozoites and cysts. Acanthamoeba genotype T4 isolated from contact lens paraphernalia and an environmental strains were propagated for ...
#1Hae-Ahm Lee (Kyung Hee University)H-Index: 12
#2Eun-Kyung Moon (Kyung Hee University)H-Index: 8
Last. Fu-Shi Quan (Kyung Hee University)H-Index: 36
view all 3 authors...
Abstract Safety precautions prior to contact lens usage is essential for preventing Acanthamoeba keratitis. Contact lens disinfecting solutions containing 3% hydrogen peroxide (H2O2) are known to exert amoebicidal effect against Acanthamoeba. Yet, these solutions need to be neutralized to prevent ocular irritation, which consequently may result in incomplete disinfection. In this study, amoebicidal effect of tert-butyl hydroperoxide (tBHP) was investigated and its efficacy was compared to those ...
1 CitationsSource
#1T. Martín-Pérez (University of Alcalá)H-Index: 4
#2Tania Lozano-Cruz (University of Alcalá)H-Index: 4
Last. Jorge Pérez-Serrano (University of Alcalá)H-Index: 16
view all 7 authors...
The genus Acanthamoeba can cause Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). The treatment of these illnesses is hampered by the existence of a resistance stage that many times causes infection relapses. In an attempt to add new agents to our chemotherapeutic arsenal against acanthamebiasis, two Acanthamoeba isolates were treated in vitro with newly synthesized biguanide dendrimers. Trophozoite viability analysis and ultrastructural studies showed that dendrimers pr...
4 CitationsSource
#1Eun-Kyung Moon (Kyung Hee University)H-Index: 8
#2Seungeun Lee (Dong-a University)H-Index: 2
Last. Hyun-Hee Kong (Dong-a University)H-Index: 23
view all 4 authors...
: Multipurpose contact lens disinfecting solutions (MPDS) are widely used to cleanse and disinfect microorganisms. However, disinfection efficacy of these MPDS against Acanthamoeba cyst remain insufficient. 2, 6-dichlorobenzonitrile (DCB), a cellulose synthesis inhibitor, is capable of increasing the amoebical effect against Acanthamoeba by inhibiting its encystation. In this study, we investigated the possibility of DCB as a disinfecting agent to improve the amoebicidal activity of MPDS against...
2 CitationsSource
#1Eun-Kyung Moon (Kyung Hee University)H-Index: 8
#2Seungeun Lee (Dong-a University)H-Index: 2
Last. Hyun-Hee Kong (Dong-a University)H-Index: 23
view all 4 authors...
Abstract Acanthamoeba keratitis is commonly encountered by contact lens wearers. Contact lens solution plays an important role in the safe use of contact lenses. The most popular products for disinfecting lenses are multipurpose disinfecting solutions (MPDS). However, almost all MPDS retailed in Korea are ineffective in killing Acanthamoeba. The objective of this study was to determine the possibility of using autophagy inhibitor chloroquine as a disinfecting agent to improve the amoebicidal act...
2 CitationsSource
#1Seung Mok Lee (UPR-RP: UPRRP College of Natural Sciences)H-Index: 2
#2Da-In LeeH-Index: 5
Last. Jong Soo LeeH-Index: 17
view all 6 authors...
Purpose:The aim of the study was to evaluate the effect of multipurpose contact lens (CL) solution (MPS) combined with autophagy inhibitors on the adhesion properties of Acanthamoeba castellanii (AC) trophozoites to silicone hydrogel CLs.Methods:AC trophozoites were inoculated onto discs trimmed fro
3 CitationsSource