Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil

Published on Oct 13, 2016in BMC Plant Biology3.497
· DOI :10.1186/S12870-016-0906-1
Zachary L. Demorest15
Estimated H-index: 15
Andrew Coffman6
Estimated H-index: 6
+ 10 AuthorsFeng Zhang155
Estimated H-index: 155
Background The ability to modulate levels of individual fatty acids within soybean oil has potential to increase shelf-life and frying stability and to improve nutritional characteristics. Commodity soybean oil contains high levels of polyunsaturated linoleic and linolenic acid, which contribute to oxidative instability – a problem that has been addressed through partial hydrogenation. However, partial hydrogenation increases levels of trans-fatty acids, which have been associated with cardiovascular disease. Previously, we generated soybean lines with knockout mutations within fatty acid desaturase 2-1A (FAD2-1A) and FAD2-1B genes, resulting in oil with increased levels of monounsaturated oleic acid (18:1) and decreased levels of linoleic (18:2) and linolenic acid (18:3). Here, we stack mutations within FAD2-1A and FAD2-1B with mutations in fatty acid desaturase 3A (FAD3A) to further decrease levels of linolenic acid. Mutations were introduced into FAD3A by directly delivering TALENs into fad2-1a fad2-1b soybean plants.
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