Databases for technical aspects of immunohistochemistry

Published on Jan 1, 2017in Journal of Toxicologic Pathology1.476
· DOI :10.1293/TOX.2016-0047
Satoshi Furukawa12
Estimated H-index: 12
,
Mika Nagaike2
Estimated H-index: 2
,
Kiyokazu Ozaki19
Estimated H-index: 19
(Setsunan University)
Sources
Abstract
: With the aims of sharing information about the technical aspects of immunohistochemistry (IHC) and making it possible to make a suitable choice of antibody for histopathological examination, this technical report describes the results of a questionnaire administered during the period of 2014 to 2015 to members of the Conference on Experimental Animal Histopathology. It also describes the immunological properties of primary antibodies (clone, supplier, catalog number, species reactivity, etc.) and the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, secondary antibody information, etc.) for a total number of 733 primary antibodies (425 kinds of primary antibody).
Figures & Tables
Download
📖 Papers frequently viewed together
2017
1 Citations
1 Citations
20122.74PLOS ONE
References1
Newest
#1José A. Ramos-Vara (Purdue University)H-Index: 31
#2Margaret A. Miller (Purdue University)H-Index: 32
Once focused mainly on the characterization of neoplasms, immunohistochemistry (IHC) today is used in the investigation of a broad range of disease processes with applications in diagnosis, prognostication, therapeutic decisions to tailor treatment to an individual patient, and investigations into the pathogenesis of disease. This review addresses the technical aspects of immunohistochemistry (and, to a lesser extent, immunocytochemistry) with attention to the antigen-antibody reaction, optimal ...
201 CitationsSource
Cited By38
Newest
Last. Nobue Itasaki (UoB: University of Bristol)H-Index: 22
view all 5 authors...
Three-dimensional (3D) cultures of cancer cells in liquid without extracellular matrix (ECM) offers in vitro models for metastasising conditions such as those in vessels and effusion. However, liquid culturing is often hindered by cell adhesiveness, which causes large cell clumps. We previously described a liquid culture material, LA717, which prevents non-clonal cell adhesion and subsequent clumping, thus allowing clonal growth of spheroids in an anchorage-independent manner. Here, we examined ...
Source
#1Takayasu Moroki (OPC: Otsuka Pharmaceutical)
#2Saori Matsuo (Chugai Pharmaceutical Co.)H-Index: 8
Last. Kiyokazu Ozaki (Setsunan University)H-Index: 19
view all 9 authors...
With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) ...
Source
#1Kengo UedaH-Index: 4
#2Akiko UedaH-Index: 2
Last. Kiyokazu Ozaki (Setsunan University)H-Index: 19
view all 3 authors...
This report describes the clinical and histopathological characteristics of a rare mixed germ-cell tumor comprising teratoma and embryonal carcinoma in the left ovary of a 10-month-old four-toed hedgehog, with chief complaints of loss of appetite and lethargy. Laparotomy revealed a swollen left ovary with small disseminated peritoneal nodules, and bilateral ovariohysterectomy was performed. The left ovary had a mature teratoma with well-differentiated fat, bone, cartilage, salivary gland, trache...
Source
#1Takuya DoiH-Index: 8
#2Takeshi KannoH-Index: 8
Last. Junko SatoH-Index: 7
view all 3 authors...
Pituitary proliferative lesions derived from the endocrine cells of the pars distalis are frequently encountered and adenomas/carcinomas are a common cause of death in standard 2-year carcinogenicity studies using various rat strains, especially Sprague-Dawley. This report describes the immunohistochemical characteristics of pituitary tumors derived from the pars distalis in rats. Prolactin (PRL)-containing tumors are the most common, with PRL/growth hormone (GH) dual positive tumor masses (PRL/...
Source
#1Nils Dennhag (Umeå University)H-Index: 2
#2Jing-Xia Liu (Umeå University)H-Index: 15
Last. Fatima Pedrosa Domellöf (Umeå University)H-Index: 10
view all 5 authors...
Purpose To study the medial rectus (MR) muscle of zebrafish (Danio rerio) with respect to the pattern of distribution of desmin and its correlation to distinct types of myofibers and motor endplates. Methods The MRs of zebrafish were examined using confocal microscopy in whole-mount longitudinal specimens and in cross sections processed for immunohistochemistry with antibodies against desmin, myosin heavy chain isoforms, and innervation markers. Desmin patterns were correlated to major myofiber ...
3 CitationsSource
We report a spontaneous case of nephroblastoma in a 26-week-old female Slc:CD(SD) rat. Macroscopically, there was a yellow mass in the left kidney that included another small yellowish-white mass. Histologically, the mass was located mainly in the cortex of the kidney. The tumor showed two distinct morphologies corresponding to the macroscopic findings: a blastemal cell dominant area (blastemal area) with primitive glomeruli and immature tubules and a columnar epithelial tubule dominant area wit...
Source
#1Aoi Kurokawa (National Agriculture and Food Research Organization)H-Index: 1
#2Yu Yamamoto (National Agriculture and Food Research Organization)H-Index: 16
Abstract Immunohistochemical method to detect avian lymphocytes is an efficient and reliable tool for accurate diagnosis, and immunological analysis of avian diseases. However, there are scarce studies reporting immunohistochemistry (IHC) using commercially available antibodies in formalin-fixed paraffin-embedded (FFPE) chicken tissues. In the present study, we established an immunohistochemical method to identify chicken T and B lymphocytes in FFPE chicken tissues using commercial antibodies ag...
1 CitationsSource
#1Kyohei Yasuno (Daiichi Sankyo)H-Index: 1
#2Eri Hamamura-Yasuno (Daiichi Sankyo)H-Index: 1
Last. Kazuhiko Mori (Daiichi Sankyo)H-Index: 9
view all 7 authors...
INTRODUCTION: Host cell proteins (HCPs) are contaminated proteins remaining after purification of biopharmaceuticals. Recent reports revealed clinical implications of HCPs in anti-drug antibody (ADA) development in patients without any inflammatory effects. Therefore, we evaluated the inflammatory effects and immunogenicity of HCPs in an in vivo study by intravitreal administration to rabbits and an in vitro THP-1 cells assay. METHODS: Escherichia coli-derived HCPs at 200 ng/eye with or without ...
3 CitationsSource
#1Kiyokazu Ozaki (Setsunan University)H-Index: 19
#2Tetsuro Matsuura (Setsunan University)H-Index: 12
Diabetic peripheral neuropathy (DPN) is a major complication of diabetes mellitus, and hypertension is considered to be a risk factor for DPN in patients with type 1 diabetes (T1DM). However, the morphological effects of hypertension on DPN are unclear. In this study, we investigated the effect of hypertension on DPN by investigating the changes in unmyelinated and myelinated nerve fibers in hypertensive rats with alloxan (AL)-induced T1DM. Thirteen-week-old WBN/Kob rats with AL-induced diabetes...
Source
#1M.A. Blomqvist (SLU: Swedish University of Agricultural Sciences)
#2C. Ley (SLU: Swedish University of Agricultural Sciences)
Last. J.M. Hanson (SLU: Swedish University of Agricultural Sciences)
view all 4 authors...
Summary Hypophysitis has been reported occasionally in dogs, with most cases resembling primary lymphocytic hypophysitis in man. Although it is generally assumed that lymphocytes are not present normally in the canine pituitary gland, few studies have investigated this hypothesis. However, lymphocytes are recognized in the pituitary gland of people and horses without signs of pituitary disease. It is unknown to what degree lymphocyte infiltration of the pituitary gland might occur as an incident...
Source