Could nitrosyl chloride be produced by human skin keratinocytes and sulfur mustard? A magnetic resonance study

Published on Jan 1, 1997
Carmen M. Arroyo1
Estimated H-index: 1
,
Alasdair J. Carmichael11
Estimated H-index: 11
,
C. A. Broomfield1
Estimated H-index: 1
Abstract
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1996
4 Authors (H. L. Meier, ..., C. M. Corun)
8 Citations
10 Citations
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#1David W. KahlerH-Index: 2
#2Carmen M. ArroyoH-Index: 12
Abstract : This technical report outlines procedures that have been developed to provide a cost effective way to produce large quantities of normal human skin fibroblast cells (NHSFs) for studying the mechanism(s) of action of chemical warfare agents (CWAs) and medical counter-measurements against CWAs. Various growth factors were added to media recommended by the supplier, American Type Culture Collection (ATCC), Manassas, Virginia, USA. Cell viability, cell differentiation and cell proliferati...
#1Dana R. Anderson (United States Army Medical Research Institute of Chemical Defense)H-Index: 25
#2Larry W. Mitcheltree (United States Army Medical Research Institute of Chemical Defense)H-Index: 4
Last. Mark B. Gold (United States Army Medical Research Institute of Chemical Defense)H-Index: 4
view all 6 authors...
Sulfur mustard (HD) is a vesicant compound that was first used as a chemical warfare agent in World War I. (Papirmeister et al. 1991). Numerous animal models have been used to study HD-induced vesication. In this article, we describe modifications of the vapor cup model of Mershon and colleagues (1990) to establish a new vapor cup model for use in neonatal mice. The need to develop this model resulted from the development of gene-targeted knockout mice that can be used to evaluate the function o...
4 CitationsSource
#1Carmen M. ArroyoH-Index: 12
#2Robert J. Schafer (ORNL: Oak Ridge National Laboratory)H-Index: 2
Last. Alasdair J. Carmichael (Armed Forces Radiobiology Research Institute)H-Index: 11
view all 5 authors...
Cytokines play a major role in both acute and chronic inflammatory processes, including those produced by sulfur mustard (2,2′-dichlorodiethyl sulfide, HD). This study describes responses of normal human epidermal keratinocytes (NHEK) to HD, defined by interleukin-1β (IL-1β), IL-6, IL-8 and tumor necrosis factor alpha (TNF-α) release. Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to measure the cytokine release in NHEK during exposure to 100 and 300 μM of HD. Ex...
64 CitationsSource
#1Jürgen Fuchs (Goethe University Frankfurt)H-Index: 19
#2Thomas Matthias Zollner (Goethe University Frankfurt)H-Index: 24
Last. Maurizio Podda (Goethe University Frankfurt)H-Index: 31
view all 4 authors...
Abstract Inflammatory skin diseases account for a large proportion of all skin disorders and constitute a major health problem worldwide. Contact dermatitis, atopic dermatitis, and psoriasis represent the most prevalent inflammatory skin disorders and share a common efferent T-lymphocyte mediated response. Oxidative stress and inflammation have recently been linked to cutaneous damage in T-lymphocyte mediated skin diseases, particularly in contact dermatitis. Insights into the pathophysiology re...
113 CitationsSource
#1Carmen M. Arroyo (DA: United States Department of the Army)H-Index: 12
#2Robert J. Schafer (ORNL: Oak Ridge National Laboratory)H-Index: 2
Last. Alasdair J. CarmichaelH-Index: 11
view all 5 authors...
Cytokines play a major role in both acute and chronic inflammatory processes, including those produced by sulfur mustard (HD). This study describes responses of normal human epidermal keratinocyte (NHEK) cells to 2,2'-dichlorodiethyl sulfide, sulfur mustard (HD), defined by interleukin-1 beta (IL-1β), interleukin-6 (IL-6], interleukin-8 (IL-8), and tumor necrosis factor-alpha (TNF-α) release. A new method for detaching cell to cell adhesion between keratinocytes has been applied. This method per...
49 CitationsSource