Original paper

A cost‐efficient and simple protocol to enrich prey DNA from extractions of predatory arthropods for large‐scale gut content analysis by Illumina sequencing

Volume: 8, Issue: 1, Pages: 126 - 134
Published: Sep 29, 2016
Abstract
Summary Molecular analysis of predator gut content is a popular tool to uncover food web structure and has greatly profited from the emergence of next‐generation sequencing technology. However, the molecular recovery of prey spectra comes with many challenges, particularly the overabundance of predator DNA in extractions. When predator and prey are distantly related, predator‐specific blocking primers can be used to preferentially amplify prey...
Paper Details
Title
A cost‐efficient and simple protocol to enrich prey DNA from extractions of predatory arthropods for large‐scale gut content analysis by Illumina sequencing
Published Date
Sep 29, 2016
Volume
8
Issue
1
Pages
126 - 134
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