Structure of fully protonated proteins by proton-detected magic-angle spinning NMR

Significance Protein structure determination is key to the detailed description of many biological processes. The critical factor that would allow general application of magic-angle spinning (MAS) solid-state NMR to this end is improvement in sensitivity and resolution for as many nuclear spins as possible. This is achieved here with detection of resolved 1 H resonances in protonated proteins by increasing MAS rates to frequencies of 100 kHz and...