Improving cytoactive of endothelial cell by introducing fibronectin to the surface of poly L-Lactic acid fiber mats via dopamine

Published on Dec 1, 2016in Materials Science and Engineering: C5.88
· DOI :10.1016/J.MSEC.2016.07.006
Wufeng Yang5
Estimated H-index: 5
(JNU: Jinan University),
Xiazhi Zhang4
Estimated H-index: 4
(JNU: Jinan University)
+ 3 AuthorsChangren Zhou34
Estimated H-index: 34
(JNU: Jinan University)
Sources
Abstract
Abstract A simple but straightforward approach was reported to prepare fiber mats modified with fibronectin (Fn) protein for endothelial cells activity study. Based on the self-polymerization and strong adhesion feature of dopamine, poly L-Lactic acid (PLLA) fibers mat was modified via simply immersing them into dopamine solution for 16 h. Subsequently, Fn was immobilized onto the fiber mats surface by the coupling reactive polydopamine (PDA) layer and Fn. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) were used to determine the chemical compositions of fiber mats surface, which confirmed the successful immobilization of PDA and Fn molecules on the fiber surface. Scanning electronic microscopy (SEM) was used to observe the surface morphology changes after modification with PDA and Fn. The data of water contact angle showed that the hydrophilicity of the fiber mats was improved after surface modification. The data of in vitro cell culture proved that the PDA and Fn modified surface significantly enhanced the adhesion, proliferation and cell activity of endothelial cells on the fiber mats. And the release of tumor necrosis factor-α (TNF-α) by endothelial cells on the modified surface was suppressed compared to that on culture plate and PLLA film at 2 and 4 days, while the secretion of interleukin-1β (IL-1β) was increased compared to that on culture plate and PLLA film at 2 days.
📖 Papers frequently viewed together
95 Citations
63 Citations
5 Citations
References33
Newest
#1Xiazhi Zhang (JNU: Jinan University)H-Index: 4
#2Rui Guo (JNU: Jinan University)H-Index: 19
Last. Yaowu Zhao (JNU: Jinan University)H-Index: 1
view all 7 authors...
In this study, poly(l-lactide) (PLLA)/halloysite nanotube (HNT) electrospun mats were prepared as a dual-drug delivery system. HNTs were used to encapsulate polymyxin B sulphate (a hydrophilic drug...
27 CitationsSource
#1Priyadharsini Karuppuswamy (NTHU: National Tsing Hua University)H-Index: 7
#1Priyadharsini Karuppuswamy (NTHU: National Tsing Hua University)H-Index: 3
Last. Seeram Ramakrishna (NUS: National University of Singapore)H-Index: 162
view all 6 authors...
Abstract Nanotechnology being one of the most promising technologies today shows an extremely huge potential in the field of tissue engineering to mimic the porous topography of natural extracellular matrix (ECM). Natural polymers are incorporated into the synthetic polymers to fabricate functionalized hybrid nanofibrous scaffolds, which improve cell and tissue compatibility. The present study identified the biopolymers – aloe vera, silk fibroin and curcumin incorporated into polycaprolactone (P...
47 CitationsSource
#1Li Jin (UVA: University of Virginia)H-Index: 15
#2Adam L. Shimmer (UVA: University of Virginia)H-Index: 1
Last. Xudong Li (UVA: University of Virginia)H-Index: 26
view all 3 authors...
Background Intervertebral disc degeneration, a main cause of back pain, is an endemic problem and a big economic burden for the health care system. Current treatments are symptom relieving but do not address underlying problems—biological and structural deterioration of the disc. Tissue engineering is an emerging approach for the treatment of intervertebral disc degeneration since it restores the functionality of native tissues. Although numerous studies have focused on the nucleus pulposus tiss...
27 CitationsSource
#1Yu-Mi Shin (UPR-RP: UPRRP College of Natural Sciences)H-Index: 1
#2Hyun-Joo Jung (Yonsei University)H-Index: 7
Last. Chang-Jin Lim (UPR-RP: UPRRP College of Natural Sciences)H-Index: 1
view all 4 authors...
Ginsenoside Rg3 is one of ginsenosides that are the well-known bioactive principles of Panax ginseng. Among the two stereoisomeric forms of Rg3, 20(S)-ginsenoside Rg3 [20(S)-Rg3] is predominant. 20(S)-Rg3 is capable of suppressing the nitric oxide (NO), reactive oxygen species (ROS) and prostaglandin E2 (PGE2) productions induced by lipopolysaccharide (LPS) in RAW264.7 macrophage cells in a concentration-dependent manner. In the same stimulated macrophages, 20(S)-Rg3 was able to suppress matrix ...
63 CitationsSource
#1Yanpeng Jiao (JNU: Jinan University)H-Index: 17
#1Yanpeng Jiao (JNU: Jinan University)H-Index: 2
Last. Changren Zhou (JNU: Jinan University)H-Index: 34
view all 4 authors...
Surface modification of biomaterials has been adopted over the years in order to improve their biocompatibility. In this study, heparin, hyaluronic acid and chondroitin sulfate were used to modify the surface of poly(L-lactic acid) (PLLA) films by an entrapment method to promote its biocompatibility and to introduce natural recognition sites to the surface of PLLA films. The surface properties of PLLA films before and after modification were characterized by using water contact angle measurement...
14 CitationsSource
#1Yanpeng Jiao (JNU: Jinan University)H-Index: 2
#1Jiao Yanpeng (JNU: Jinan University)H-Index: 4
Last. Changren Zhou (JNU: Jinan University)H-Index: 34
view all 3 authors...
Ammonia plasma treatment is an efficient method to modify the surface of polymeric biomaterials to improve their hydrophilicity and biocompatibility. In this study, poly(L-lactic acid) (PLLA) films were treated with ammonia plasma to investigate the effects on the surface properties and cytocompatibility. Surface morphologies of the films were observed with atomic force microscopy (AFM) and the surface roughness was analyzed with the software attached to the AFM. Mass loss density and contact an...
12 CitationsSource
#1An Du (Drexel University)H-Index: 5
#2Donghun Koo (Drexel University)H-Index: 5
Last. Richard A. Cairncross (Drexel University)H-Index: 20
view all 5 authors...
Abstract Polylactide is a bio-based and biodegradable polymer well-known for its renewable origins. Water sorption and clustering behavior in both a homopolymer polylactide and a graft copolymer of polylactide was studied using the quartz crystal microbalance/heat conduction calorimetry (QCM/HCC) technique. The graft copolymer, poly(1,5-cyclooctadiene-co-5-norbornene-2-methanol- graft - d,l -lactide), contained polylactide chains (95 wt.%) grafted onto a hydrophobic rubbery backbone (5 wt.%). Cl...
19 CitationsSource
#1Fengyi Du (NKU: Nankai University)H-Index: 3
#2Hao Wang (NKU: Nankai University)H-Index: 106
Last. Yuanyuan Zhang (Wake Forest Institute for Regenerative Medicine)H-Index: 50
view all 7 authors...
Abstract One of the major challenges of tissue-engineered small-diameter blood vessels is restenosis caused by thrombopoiesis. The goal of this study was to develop a 3D gradient heparinized nanofibrous scaffold, aiding endothelial cells lined on the lumen of blood vessel to prevent thrombosis. The vertical graded chitosan/poly ɛ-caprolactone (CS/PCL) nanofibrous vessel scaffolds were fabricated with chitosan and PCL by sequential quantity grading co-electrospinning. To mimic the natural blood v...
150 CitationsSource
#1Jiqing Xu (JNU: Jinan University)H-Index: 3
#2Jiao Yanpeng (JNU: Jinan University)H-Index: 4
Last. Changren Zhou (JNU: Jinan University)H-Index: 4
view all 4 authors...
Abstract This work is to develop novel electrospun poly( l -lactic acid) (PLLA) fiber mats for controllable delivery of hydrophobic and hydrophilic drugs. For this aim, bovine serum albumin (BSA, used as a hydrophilic model drug) was firstly enveloped into chitosan microspheres by spray drying. Benzoin (used as a hydrophobic model drug) was directly dissolved in PLLA solution and then the chitosan microspheres were suspended into the PLLA solution, which was used to prepare PLLA fiber mats by el...
55 CitationsSource
#1Si Chen (WHU: Wuhan University)H-Index: 22
#2Si-Xue Cheng (WHU: Wuhan University)H-Index: 68
Last. Ren-Xi Zhuo (WHU: Wuhan University)H-Index: 82
view all 3 authors...
Nanoparticulate drug-delivery systems have attained much importance because of their injectable property, the possibility to achieve passive targeting and active targeting, and unique advantages to realize stimuli tailored delivery. Molecular self-assembly is a powerful method for fabricating polymer-based nanoparticles, which involves various driving forces, such as hydrophobic interactions, electrostatic interactions, stereo-complexation, host/guest interactions and hydrogen bonding. By fine t...
63 CitationsSource
Cited By13
Newest
#1Zhenzhao GuoH-Index: 1
#1Zhenzhao Guo (Guangzhou Medical University)
Last. Zhizhong LiH-Index: 2
view all 8 authors...
An innovative basic fibroblast growth factor (bFGF)-loaded polycaprolactone (PCL) fibrous membrane with highly aligned structure is developed for guided tissue regeneration (GTR). The aligned membrane is fabricated by electrospinning. In order to make efficient use of bFGF, PCL electrospun fibrous membrane is firstly surface-coated by self-polymerization of dopamine, and followed by immobilization of heparin via covalent conjugation to the polydopamine (PDA) layer. Subsequently, bFGF is loaded b...
Source
#1Chiyu Wen (TJU: Tianjin University)H-Index: 7
#2Jiamin Zhang (Peking Union Medical College)H-Index: 3
Last. Lei Zhang (TJU: Tianjin University)H-Index: 25
view all 12 authors...
Coronary stent implantation is an effective procedure for percutaneous coronary intervention treatment. However, its long-term safety and efficacy are still hindered by the in-stent restenosis and late thrombus formation. Herein, an anti-biofouling and endothelial cell selective zwitterionic hydrogel coating was developed to simultaneously enhance the nonspecific resistance and rapid re-endothelialization of the titanium surface. An endothelial cell selective peptide, REDV, could be simply conju...
Source
#1Ling Zhu (JNU: Jinan University)H-Index: 4
#2Shitian Chen (JNU: Jinan University)H-Index: 4
Last. Binghong Luo (JNU: Jinan University)H-Index: 17
view all 8 authors...
Abstract We present here a porous poly (L-lactide)/chitosan (PLLA/CS) composite scaffold with micro/nano-fiber hierarchical structure that was innovatively fabricated by combining 3D printing and thermally induced phase separation technology. Moreover, bioactive quercetin (Qu) was chosen to further functionalize the PLLA/CS scaffold with polydopamine (PDA) layer. The final results showed that the hydrophilicity and mechanical properties of the resulting PLLA/CS-D/Qu scaffold were obviously super...
13 CitationsSource
#1Anton ManakhovH-Index: 18
Last. Anastasiya O. SolovievaH-Index: 12
view all 8 authors...
The immobilization of viable proteins is an important step in engineering efficient scaffolds for regenerative medicine. For example, angiogenin, a vascular growth factor, can be considered a neurotrophic factor, influencing the neurogenesis, viability, and migration of neurons. Angiogenin shows an exceptional combination of angiogenic, neurotrophic, neuroprotective, antibacterial, and antioxidant activities. Therefore, this protein is a promising molecule that can be immobilized on carriers use...
1 CitationsSource
#1Guijuan Han (JNU: Jinan University)H-Index: 2
#2Liu Shuying (JNU: Jinan University)H-Index: 1
Last. Changren Zhou (JNU: Jinan University)H-Index: 34
view all 9 authors...
Abstract In this study, a poly( d , l -lactide) (PDLLA) membrane was prepared by the solution casting method, then the surface of the membrane was modified by polydopamine (PDOPA) as a substrate, followed by adsorption of different chitosan derivative sulfonated chitosan (SCS) or/and phosphorylated chitosan (PCS) to obtain different functionalized membranes, and two kinds of chitosan derivatives characterized by FTIR, elemental analysis and zeta potential. And different membranes were evaluated ...
9 CitationsSource
#1Yufen Wu (Donghua University)H-Index: 3
#2Chenglong Yu (Donghua University)H-Index: 6
Last. Guoping Guan (Donghua University)H-Index: 8
view all 5 authors...
: Developing a small-diameter vascular graft with a satisfactory performance in terms of mechanical and biological properties remains a challenging issue because of comprehensive requirements from clinical applications. Polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels exhibit many desirable characteristics for small-diameter vascular grafts because of their tunable mechanical properties, especially high compliance. However, poor cells adhesion hinders their application for endothelializat...
6 CitationsSource
#1Hugo G. Lemos (UFABC: Universidade Federal do ABC)H-Index: 3
#2Luis Marcelo Garcia da Silva (UFABC: Universidade Federal do ABC)H-Index: 1
Last. Everaldo C. Venancio (UFABC: Universidade Federal do ABC)H-Index: 16
view all 6 authors...
Abstract The combination of scaffolds with desirable topographic characteristics and the use of electrical stimulus consist of a strategy to repair and regenerate tissues. An interesting approach to obtain electroactive scaffolds with the aforementioned features comprises on the use of conducting polymers which can be blended with other biocompatible polymers. In this work, poly( l -lactic acid) (PLLA) and poly(ortho-ethoxyaniline) (POEA) were synthesized and PLLA/POEA mats were prepared for the...
1 CitationsSource
#1Tengjiaozi Fang (PKU: Peking University)H-Index: 4
#2Zuoying Yuan (Huada: Beijing University of Chemical Technology)H-Index: 8
Last. Qing Cai (Huada: Beijing University of Chemical Technology)H-Index: 26
view all 10 authors...
Abstract To provide a suitable 3D microenvironment for bone regeneration, we selected the injectable poly (l-lactic acid) nanofibrous microspheres (PLLA NF-Ms) with different surface modifications to serve as cell micro-carriers or protein vehicles on-demand. Results showed that polydopamine (PDA) modified NF-Ms (PDA-NF-Ms) exhibited good affinity for stem cells from human exfoliated deciduous teeth (SHED), and Heparin-Dopamine (Hep-Dopa) conjugated with NF-Ms (Hep-Dopa NF-Ms) are able to immobi...
7 CitationsSource
#1Yong Liu (Soochow University (Suzhou))H-Index: 1
#2Changlu Xu (Soochow University (Suzhou))H-Index: 5
Last. Liang Chen (Soochow University (Suzhou))H-Index: 18
view all 7 authors...
It is highly desirable for bone tissue engineering scaffolds to have significant osteogenic properties and capability to improve cell growth and thus enhance bone regeneration. In this study, a poly(L-lactic acid) (PLLA) nanofiber scaffold-immobilized osteogenic growth peptide (OGP) was prepared via polydopamine (PDA) coating. X-ray photoelectron spectroscopy (XPS), contact angle measurement, and scanning electron microscopy (SEM) were used to determine the OGP immobilization, hydrophilicity and...
10 CitationsSource
#1Keke Wu (JNU: Jinan University)H-Index: 9
#2Xi Song (JNU: Jinan University)H-Index: 5
Last. Changren Zhou (JNU: Jinan University)H-Index: 34
view all 6 authors...
Abstract Hyperbilirubinemia could be treated effectively by hemoperfusion. Electrospinning fiber mat had been reported to be applied in the hemoperfusion field owing to its higher surface area. BSA, as one of the natural carrier proteins for bilirubin, can not only selectively adsorb bilirubin but also improve the hemocompatibility of biomaterials. In this work, functionalized polyethersulfone (PES) electrospun fiber mat was prepared by immobilizing BSA on the surface to remove bilirubin efficie...
12 CitationsSource