Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62
Abstract
SQSTM1 is an adaptor protein that integrates multiple cellular signaling pathways and whose expression is tightly regulated at the transcriptional and post-translational level. Here, we describe a forward genetic screening paradigm exploiting CRISPR-mediated genome editing coupled to a cell selection step by FACS to identify regulators of SQSTM1. Through systematic comparison of pooled libraries, we show that CRISPR is superior to RNAi in...
Paper Details
Title
Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62
Published Date
Jun 28, 2016
Journal
Volume
5
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