Preparation and properties of PLGA nanofiber membranes reinforced with cellulose nanocrystals.

Published on Aug 1, 2015in Colloids and Surfaces B: Biointerfaces4.389
· DOI :10.1016/J.COLSURFB.2015.05.029
Yunfei Mo6
Estimated H-index: 6
(JNU: Jinan University),
Rui Guo19
Estimated H-index: 19
(JNU: Jinan University)
+ 4 AuthorsYuanming Zhang15
Estimated H-index: 15
(JNU: Jinan University)
Sources
Abstract
Abstract Although extensively used in the fields of drug-carrier and tissue engineering, the biocompatibility and mechanical properties of polylactide–polyglycolide (PLGA) nanofiber membranes still limit their applications. The objective of this study was to improve their utility by introducing cellulose nanocrystals (CNCs) into PLGA nanofiber membranes. PLGA and PLGA/CNC composite nanofiber membranes were prepared via electrospinning, and the morphology and thermodynamic and mechanical properties of these nanofiber membranes were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and dynamic mechanical analysis (DMA). The cytocompatibility and cellular responses of the nanofiber membranes were also studied by WST-1 assay, SEM, and confocal laser scanning microscopy (CLSM). Incorporation of CNCs (1, 3, 5, and 7 wt.%) increased the average fiber diameter of the prepared nanofiber membranes from 100 nm (neat PLGA) to ∼400 nm (PLGA/7 wt.% CNC) and improved the thermal stability of the nanofiber membranes. Among the PLGA/CNC composite nanofiber membranes, those loaded with 7 wt.% CNC nanofiber membranes had the best mechanical properties, which were similar to those of human skin. Cell culture results showed that the PLGA/CNC composite nanofiber membranes had better cytocompatibility and facilitated fibroblast adhesion, spreading, and proliferation compared with neat PLGA nanofiber membranes. These preliminary results suggest that PLGA/CNC composite nanofiber membranes are promising new materials for the field of skin tissue engineering.
📖 Papers frequently viewed together
3,928 Citations
50 Citations
683 Citations
References44
Newest
#1Weichang Li (JNU: Jinan University)H-Index: 5
#2Yong Lan (JNU: Jinan University)H-Index: 14
Last. Yuanming Zhang (JNU: Jinan University)H-Index: 15
view all 6 authors...
Tissue-engineered dermis is thought to be the best treatment for skin defects; however, slow vascularization of these biomaterial scaffolds limits their clinical application. Exogenous administration of angiogenic growth factors is highly desirable for tissue regeneration. In this study, biodegradable gelatin microspheres (GMs) containing basic fibroblast growth factor (bFGF) were fabricated and incorporated into a porous collagen/cellulose nanocrystals (CNCs) scaffold, as a platform for long-te...
40 CitationsSource
#1Farah Hanani Zulkifli (Universiti Malaysia Pahang)H-Index: 7
#2Fathima Shahitha Jahir Hussain (Universiti Malaysia Pahang)H-Index: 10
Last. Mashitah M. Yusoff (Universiti Malaysia Pahang)H-Index: 38
view all 4 authors...
In this study, a novel fibrous membrane of hydroxyethyl cellulose (HEC)/poly(vinyl alcohol) blend was successfully fabricated by electrospinning technique and characterized. The concentration of HEC (5%) with PVA (15%) was optimized, blended in different ratios (30–50%) and electrospun to get smooth nanofibers. Nanofibrous membranes were made water insoluble by chemically cross-linking by glutaraldehyde and used as scaffolds for the skin tissue engineering. The microstructure, morphology, mechan...
34 CitationsSource
#1Larissa Morais Ribeiro da Silva (UFC: Federal University of Ceará)H-Index: 4
#2Laura E. Hill (A&M: Texas A&M University)H-Index: 10
Last. Carmen L. Gomes (A&M: Texas A&M University)H-Index: 29
view all 4 authors...
Nanoencapsulation offers great potential in natural compounds delivery as it protects them from degradation, improves their aqueous solubility, and delivers active compounds to the action site. Poly (dl-lactide-co-glycolide) (PLGA) nanoparticles of acerola, guava, and passion fruit by-product extracts were synthesized using the emulsion–evaporation method. PLGA with different lactide to glycolide (50:50 and 65:35) ratios were used to determine how polymer composition affected nanoparticles prope...
51 CitationsSource
#1Ying Mu (SJTU: Shanghai Jiao Tong University)H-Index: 1
#2Fei WuH-Index: 22
Last. Weien YuanH-Index: 29
view all 5 authors...
Nerve tissue regeneration approaches have gained much attention in recent years, and nerve conduits (NCs), which facilitate nerve tissue regeneration, have become an attractive alternative to nerve autologous graft. Several methods are proposed to fabricate NCs, including electrospinning, which is a widely used approach for NCs and other tissue scaffolds, and has advantages such as the ability to control the thickness, diameter and porosity of fibers, as well as its simple experimental set up. T...
22 CitationsSource
#1Raffaella Casasola (Lboro: Loughborough University)H-Index: 2
#2Noreen L. Thomas (Lboro: Loughborough University)H-Index: 27
Last. Stella Georgiadou (Lboro: Loughborough University)H-Index: 10
view all 4 authors...
The selection of an appropriate non-hazardous solvent or solvent system is essential to determine the rheological properties and electrospinnability of the solution, the productivity, and the morphology of nanofibres. In this study, poly lactic acid (PLA) solutions were prepared in various pure solvents and binary-solvent systems to investigate the effect of different solution properties on nanofibre morphology and diameter. Viscosity, conductivity and surface tension of each solution were measu...
157 CitationsSource
#1Rui M. A. Domingues (University of Minho)H-Index: 19
#2Manuela E. GomesH-Index: 61
Last. Rui L. ReisH-Index: 129
view all 3 authors...
Cellulose nanocrystals (CNCs) are a renewable nanosized raw material that is drawing a tremendous level of attention from the materials community. These rod-shaped nanocrystals that can be produced from a variety of highly available and renewable cellulose-rich sources are endowed with exceptional physicochemical properties which have promoted their intensive exploration as building blocks for the design of a broad range of new materials in the past few decades. However, only recently have these...
231 CitationsSource
#1Jiashing Yu (NTU: National Taiwan University)H-Index: 19
#2An-Rei LeeH-Index: 1
Last. Wei-Bor TsaiH-Index: 41
view all 6 authors...
Structural similarity of electrospun fibers (ESFs) to the native extracellular matrix provides great potential for the application of biofunctional ESFs in tissue engineering. This study aimed to synthesize biofunctionalized poly (L-lactide-co-glycolide) (PLGA) ESFs for investigating the potential for cardiac tissue engineering application. We developed a simple but novel strategy to incorporate adhesive peptides in PLGA ESFs. Two adhesive peptides derived from laminin, YIGSR, and RGD, were cova...
62 CitationsSource
#1Chong Wang (HKU: University of Hong Kong)H-Index: 15
#2Min Wang (HKU: University of Hong Kong)H-Index: 79
Tissue engineering holds great promises in providing successful treatments of human body tissue loss that current methods are unable to treat or unable to achieve satisfactory clinical outcomes. In scaffold-based tissue engineering, a highperformance scaffold underpins the success of a tissue engineering strategy and a major direction in the field is to create multifunctional tissue engineering scaffolds for enhanced biological performance and for regenerating complex body tissues. Electrospinni...
20 CitationsSource
#1Weichang Li (JNU: Jinan University)H-Index: 5
#2Rui Guo (JNU: Jinan University)H-Index: 19
Last. Yuanming Zhang (JNU: Jinan University)H-Index: 15
view all 6 authors...
Collagen films have been widely used in the field of biomedical engineering. However, the poor mechanical properties of collagen have limited its application. Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films. A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10. The morphology of the resulting films was analyzed by scanning electron mi...
50 CitationsSource
#1Paul P. Bonvallet (UAB: University of Alabama at Birmingham)H-Index: 7
Last. Susan L. BellisH-Index: 46
view all 6 authors...
The goal of this study was to synthesize skin substitutes that blend native extracellular matrix (ECM) molecules with synthetic polymers which have favorable mechanical properties. To this end, scaffolds were electrospun from collagen I (col) and poly(ɛ-caprolactone) (PCL), and then pores were introduced mechanically to promote fibroblast infiltration, and subsequent filling of the pores with ECM. A 70:30 col/PCL ratio was determined to provide optimal support for dermal fibroblast growth, and a...
29 CitationsSource
Cited By51
Newest
Last. Sangeetha DharmalingamH-Index: 16
view all 4 authors...
Source
#1Nicola Ciarfaglia (Paul Sabatier University)
#2Antonio LaezzaH-Index: 6
Last. Brigida BochicchioH-Index: 21
view all 0 authors...
Source
3 CitationsSource
#1Tobias Kuhnt (UM: Maastricht University)H-Index: 4
#2Sandra Camarero-Espinosa (Ikerbasque)H-Index: 12
Abstract Cellulose nanomaterials (CNMs) have attracted great attention in the last decades due to the abundance of the biopolymer, the biorenewable character and the outstanding mechanical properties they account for. These, together with their biocompatibility makes them ideal candidates for tissue engineering (TE) applications. Additive manufacturing is an ideal biofabrication approach for TE, providing rapid and reliable technologies to produce scaffolds aimed for the guidance of host or impl...
1 CitationsSource
#1Jianhang Jiao (JLU: Jilin University)H-Index: 1
#2Chuangang Peng (JLU: Jilin University)H-Index: 7
Last. Su Pan (JLU: Jilin University)H-Index: 7
view all 6 authors...
Electrospun fibrous scaffolds combined with bioactive factors can display impressive performance as an ideal wound dressing, since they can mimic the composition and physicochemical properties of the extracellular matrix (ECM). The aim of this study was to fabricate a new composite biomaterial (IGF1-DA and Os-DA-modified PLGA electrospun fibrous scaffold) for wound healing, using a rat model for experimental evaluation. A small pentapeptide tag composed of DA-Lys-DA-Lys-DA residues was introduce...
Source
#1Ahmad HivechiH-Index: 7
#2S. Hajir Bahrami (AUT: Amirkabir University of Technology)H-Index: 21
Last. Sara Simorgh (IUMS: Iran University of Medical Sciences)H-Index: 4
view all 9 authors...
Abstract Mechanical properties of tissue engineering nanofibrous scaffolds are of importance because they not only determine their ease of application, but also influence the environment for cell growth and proliferation. Cellulose nanocrystals (CNCs) are natural renewable nanoparticles that have been widely used for manipulating nanofibers' mechanical properties. In this article, cellulose nanoparticles were incorporated into poly(caprolactone) (PCL) solution, and composite nanofibers were prod...
2 CitationsSource
#1Chaoling Bao (Hainan Normal University)H-Index: 3
#2Xiuqiong Chen (Hainan Normal University)H-Index: 9
Last. Qiang Lin (Hainan Normal University)H-Index: 10
view all 8 authors...
Abstract The cellulose nanocrystals (CNCs) synthesized by sulfuric acid hydrolysis are highly hydrophilic and prone to form the agglomerations due to the hydrogen bonding, which limit their applications in the formulations of Pickering emulsions. For this reason, we selected microcrystalline cellulose (MCC) as the raw material for the extraction of CNCs through sulfuric acid hydrolysis and hydrogen peroxide oxidation, and then combined the surfactants of cetyltrimethylammonium bromide (CTAB) and...
3 CitationsSource
#1Xiaoyu Gong (U of A: University of Alberta)H-Index: 6
#1Xiaoyu Gong (U of A: University of Alberta)
Last. Yaman Boluk (U of A: University of Alberta)H-Index: 24
view all 4 authors...
Cellulose nanocrystals (CNCs) with high specific surface area, crystallinity, aspect ratio with unique rod-like shapes, elastic modulus and tensile strength are an excellent additive which can be u...
Source
#1Nicola Ciarfaglia (University of Basilicata)H-Index: 1
#2Antonietta Pepe (University of Basilicata)H-Index: 21
Last. Brigida Bochicchio (University of Basilicata)H-Index: 21
view all 7 authors...
In this work, we produced cross-linked electrospun hybrid scaffolds composed of gelatin/poly-d,l-lactide (PDLLA), gelatin/PDLLA/nanocellulose, and gelatin/PDLLA/cellulose nanocrystals/elastin. Four...
Source
#1Vanessa Oliveira Castro (UFSC: Universidade Federal de Santa Catarina)H-Index: 1
#2Márcio Celso Fredel (UFSC: Universidade Federal de Santa Catarina)H-Index: 19
Last. Claudia Merlini (UFSC: Universidade Federal de Santa Catarina)H-Index: 14
view all 6 authors...
Abstract Guided bone regeneration (GBR) have been proved great success for the treatment of bone defects in oral implantology. Bone defects are reconstructed by the use of a barrier membrane which prevents the invasion of soft tissue and create a space for guiding new bone growth into the bone defect. To develop GBR membranes with higher bioactivity, poly (lactic-co-glycolic acid) (PLGA) fibrous membranes containing β-tricalcium phosphate (β-TCP) were fabricated via electrospinning. The presence...
4 CitationsSource