Reconstruction of epidermis on a chitosan cross/linked collagen/GAG lattice: effect of fibroblasts.

Reconstruction of epidermis on a fibroblast containing chitosan cross/linked collagen/GAG lattice at the air/liquid interface gives rise to a multilayered stratified epithelium, covered with a compact stratum corneum. Immunohistological studies reveal that the markers of epidermal differentiation are essentially distributed as in normal human skin and that the major proteins of the dermal/epidermal junction are present. Reconstruction of...