Murine fundus fluorescein angiography: An alternative approach using a handheld camera

Published on Jul 1, 2016in Experimental Eye Research3.011
· DOI :10.1016/J.EXER.2016.05.024
Moshe Ehrenberg4
Estimated H-index: 4
(HUJI: Hebrew University of Jerusalem),
Scott Ehrenberg1
Estimated H-index: 1
(Rappaport Faculty of Medicine)
+ 1 AuthorsOfra Benny22
Estimated H-index: 22
(HUJI: Hebrew University of Jerusalem)
Sources
Abstract
Abstract In today’s modern pharmacologic approach to treating sight-threatening retinal vascular disorders, there is an increasing demand for a compact, mobile, lightweight and cost-effective fluorescein fundus camera to document the effects of antiangiogenic drugs on laser-induced choroidal neovascularization (CNV) in mice and other experimental animals. We have adapted the use of the Kowa Genesis Df Camera to perform Fundus Fluorescein Angiography (FFA) in mice. The 1 kg, 28 cm high camera has built-in barrier and exciter filters to allow digital FFA recording to a Compact Flash memory card. Furthermore, this handheld unit has a steady Indirect Lens Holder that firmly attaches to the main unit, that securely holds a 90 diopter lens in position, in order to facilitate appropriate focus and stability, for photographing the delicate central murine fundus. This easily portable fundus fluorescein camera can effectively record exceptional central retinal vascular detail in murine laser-induced CNV, while readily allowing the investigator to adjust the camera’s position according to the variable head and eye movements that can randomly occur while the mouse is optimally anesthetized. This movable image recording device, with efficiencies of space, time, cost, energy and personnel, has enabled us to accurately document the alterations in the central choroidal and retinal vasculature following induction of CNV, implemented by argon-green laser photocoagulation and disruption of Bruch’s Membrane, in the experimental murine model of exudative macular degeneration.
References20
Newest
#1Mark C. Butler (UB: University at Buffalo)H-Index: 12
#2Jack M. Sullivan (UB: University at Buffalo)H-Index: 11
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#2Johanna MeyerH-Index: 5
view all 7 authors...
Purpose: To investigate the use of imaging and quantitative measurement capabilities of a modified fundus camera in a rat model of laser-induced choroidal neovascularization. Methods: Following induction of experimental choroidal neovascularization, Dark Agouti rats underwent serial in vivo imaging with a fundus camera (FF450plus, Carl Zeiss MediTec, Jena, Germany), including color, reflectance and fluorescence imaging. Results: A custom-made setting allowed high-resolution imaging. Change of fl...
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#2Mario R. Romano (UNIMOL: University of Molise)H-Index: 16
Last. Adolfo Sebastiani (University of Ferrara)H-Index: 22
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Age-related macular degeneration (AMD) is a multifactorial disease that represents the most common cause of irreversible visual impairment among people over the age of 50 in Europe, the United States, and Australia, accounting for up to 50% of all cases of central blindness. Risk factors of AMD are heterogeneous, mainly including increasing age and different genetic predispositions, together with several environmental/epigenetic factors, that is, cigarette smoking, dietary habits, and phototoxic...
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#2David A. Copland (UoB: University of Bristol)H-Index: 49
Last. Heping Xu ('QUB': Queen's University Belfast)H-Index: 55
view all 7 authors...
Neovascular retinal disease is a leading cause of blindness orchestrated by inflammatory responses. Although noninfectious uveoretinitis is mediated by CD4 + T cells, in the persistent phase of disease, angiogenic responses are observed, along with degeneration of the retina. Full clinical manifestation relies on myeloid-derived cells, which are phenotypically distinct from, but potentially sharing common effector responses to age-related macular degeneration. To interrogate inflammation-mediate...
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#1T Michael Nork (UW: University of Wisconsin-Madison)H-Index: 18
Last. Stanley J. WiegandH-Index: 81
view all 8 authors...
Objective To evaluate the efficacy of systemic and intravitreous administration of VEGF Trap (aflibercept) in a nonhuman primate model of choroidal neovascularization (CNV). Methods VEGF Trap treatment on laser-induced CNV was evaluated in 48 adult cynomolgus monkeys. In the prevention arms of the study, VEGF Trap was administered by intravenous injection (3 or 10 mg/kg weekly) or intravitreous injection (50, 250, or 500 μg/eye every 2 weeks) beginning before laser injury. In the treatment arm, ...
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#1Ofra Benny (Boston Children's Hospital)H-Index: 22
#2Kei Nakai (Boston Children's Hospital)H-Index: 14
Last. Robert J. D'Amato (Boston Children's Hospital)H-Index: 60
view all 10 authors...
Pathological neovascularization is a hallmark of late stage neovascular (wet) age-related macular degeneration (AMD) and the leading cause of blindness in people over the age of 50 in the western world. The treatments focus on suppression of choroidal neovascularization (CNV), while current approved therapies are limited to inhibiting vascular endothelial growth factor (VEGF) exclusively. However, this treatment does not address the underlying cause of AMD, and the loss of VEGF's neuroprotective...
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#1David M. Brown (Houston Methodist Hospital)H-Index: 46
Last. Tsontcho Ianchulev (Genentech)H-Index: 20
view all 6 authors...
Objective The 2-year, phase III trial designated Anti -vascular endothelial growth factor (VEGF) Antibody for the Treatment of Predominantly Classic Chor oidal Neovascularization (CNV) in Age-related Macular Degeneration (ANCHOR) compared ranibizumab with verteporfin photodynamic therapy (PDT) in treating predominantly classic CNV. Design Multicenter, international, randomized, double-masked, active-treatment-controlled clinical trial. Participants Patients with predominantly classic, subfoveal ...
1,342 CitationsSource
#1Xiangtian Zhou (WMU: Wenzhou Medical College)H-Index: 33
#2Meixiao Shen (WMU: Wenzhou Medical College)H-Index: 31
Last. Fan Lu (WMU: Wenzhou Medical College)H-Index: 49
view all 8 authors...
PURPOSE. To investigate refraction, corneal curvature, axial components, and the correlations between the refraction and ocular growth during the emmetropization in the C57BL/6 mouse. METHODS. Ten groups of 10 mice underwent ocular measurements at 22 to 102 days after birth. Refraction was measured by photoretinoscopy and corneal radius of curvature (CRC) was measured by keratometry. Corneal thickness (CT), anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), retinal ...
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#1Ofra Benny (Boston Children's Hospital)H-Index: 22
#2Ofer Fainaru (Boston Children's Hospital)H-Index: 16
Last. Judah Folkman (Boston Children's Hospital)H-Index: 184
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