[A study on effect of bFGF, EGF and NGF on growth of cultured human corneal endothelial cells].

PURPOSE To investigate effect of bFGF, EGF and NGF on growth of cultured human corneal endothelial cells. METHODS Cultured human corneal endothelial cells were seeded into individual wells of 96-well tissue culture plate with the same culture media containing separately bFGF, EGF or NGF with a serial of concentrations of 0 ng/ml, 1 ng/ml, 3 ng/ml, 10 ng/ml, 30 ng/ml and 100 ng/ml and then cultured for 5 days. Then MTT method was used to detect...