Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation.

Published on May 16, 2016in Genomics data
· DOI :10.1016/J.GDATA.2016.05.007
Yukihiro Furusawa22
Estimated H-index: 22
(University of Toyama),
Qing-Li Zhao33
Estimated H-index: 33
(University of Toyama)
+ 4 AuthorsTakashi Kondo57
Estimated H-index: 57
(University of Toyama)
Sources
Abstract
Radiation exposure such as A-bomb or radiation therapy is considered a major health-risk factor for cardiovascular disease. In order to understand the molecular mechanisms underlying the inflammatory reaction frequently encountered in the vascular system after exposure to ionizing radiation, we carried out a global scale microarray and computational gene expression analyses on human umbilical endothelial cells (HUVECs) exposed to X-ray (2.5 Gy). The gene ontology analysis revealed that the down-regulated genes were associated with cell cycle regulation, whereas the up-regulated genes were associated with inflammatory responses, in particular, the type 1 interferon response. The computational analysis using ingenuity pathway analysis also identified a gene network containing the interferon response factor 7 (IRF7) and its transcriptional targets such as interferon-induced transcripts (IFITs) and Mx1, which have been known to be associated with inflammation in endothelial cells. The up-regulated genes and the gene network identified here may explain the inflammatory response induced by X-irradiation. These findings uncover part of the molecular basis of the mechanism(s) of the inflammatory disorder in response to X-irradiation in HUVECs. The dataset is publicly available at the Gene Expression Omnibus (GEO) repository (http://www.ncbi.nlm.nih.gov/geo/) with accession number {"type":"entrez-geo","attrs":{"text":"GSE76484","term_id":"76484"}}GSE76484.
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#2Takashi Kondo (University of Toyama)H-Index: 57
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#1Yuuki ObataH-Index: 22
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#1Yoshiaki Tabuchi (University of Toyama)H-Index: 30
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#1Helen M. Lazear (WashU: Washington University in St. Louis)H-Index: 28
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