Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation.

Published on May 16, 2016in Genomics data
· DOI :10.1016/J.GDATA.2016.05.007
Yukihiro Furusawa22
Estimated H-index: 22
(University of Toyama),
Qing-Li Zhao33
Estimated H-index: 33
(University of Toyama)
+ 4 AuthorsTakashi Kondo57
Estimated H-index: 57
(University of Toyama)
Radiation exposure such as A-bomb or radiation therapy is considered a major health-risk factor for cardiovascular disease. In order to understand the molecular mechanisms underlying the inflammatory reaction frequently encountered in the vascular system after exposure to ionizing radiation, we carried out a global scale microarray and computational gene expression analyses on human umbilical endothelial cells (HUVECs) exposed to X-ray (2.5 Gy). The gene ontology analysis revealed that the down-regulated genes were associated with cell cycle regulation, whereas the up-regulated genes were associated with inflammatory responses, in particular, the type 1 interferon response. The computational analysis using ingenuity pathway analysis also identified a gene network containing the interferon response factor 7 (IRF7) and its transcriptional targets such as interferon-induced transcripts (IFITs) and Mx1, which have been known to be associated with inflammation in endothelial cells. The up-regulated genes and the gene network identified here may explain the inflammatory response induced by X-irradiation. These findings uncover part of the molecular basis of the mechanism(s) of the inflammatory disorder in response to X-irradiation in HUVECs. The dataset is publicly available at the Gene Expression Omnibus (GEO) repository ( with accession number {"type":"entrez-geo","attrs":{"text":"GSE76484","term_id":"76484"}}GSE76484.
Figures & Tables
#1Kimimasa Sakata (University of Toyama)H-Index: 6
#2Takashi Kondo (University of Toyama)H-Index: 57
Last. Yuichi Hattori (University of Toyama)H-Index: 55
view all 10 authors...
Abstract Vascular endothelial cells can absorb higher radiation doses than any other tissue in the body, and post-radiation impaired endothelial nitric oxide synthase (eNOS) function may be developed as a potential contributor to the pathogenesis of vascular injury. In this study, we investigated early alterations of eNOS signaling in human umbilical venous endothelial cells (HUVECs) exposed to X-ray radiation. We found that ionizing radiation increased eNOS phosphorylation at Ser-1177 and depho...
#1Partha Pratim Patra (Indian Institute of Science Education and Research, Pune)H-Index: 9
#2Rohit Chikkaraddy (Indian Institute of Science Education and Research, Pune)H-Index: 21
Last. G. V. Pavan Kumar (Indian Institute of Science Education and Research, Pune)H-Index: 19
view all 5 authors...
Plasmonic hot-spot generation in solution is not reversible for single-molecule surface-enhanced Raman scattering, which limits its applications. Patra et al. tackle this problem by integrating this technique with thermo-plasmon-assisted reconfiguration of nanoparticles at a metal–fluid interface.
#1Berthe M.P. Aleman (NKI-AVL: Netherlands Cancer Institute)H-Index: 51
#2E.C. Moser (Champalimaud Foundation)H-Index: 9
Last. Sarah C. Darby (Clinical Trial Service Unit)H-Index: 83
view all 8 authors...
Improvements in treatment and earlier diagnosis have both contributed to increased survival for many cancer patients. Unfortunately, many treatments carry a risk of late effects including cardiovascular diseases (CVDs), possibly leading to significant morbidity and mortality. In this paper we describe current knowledge of the cardiotoxicity arising from cancer treatments, outline gaps in knowledge, and indicate directions for future research and guideline development, as discussed during the 201...
#1Yuuki ObataH-Index: 22
#2Yukihiro Furusawa (UTokyo: University of Tokyo)H-Index: 22
Last. Koji Hase (UTokyo: University of Tokyo)H-Index: 55
view all 22 authors...
Regulatory T cells proliferate robustly in gut lymphoid tissues. Hase et al. show that this proliferation requires their expression of the epigenetic regulator Uhrf1, which is increased in response to IL-2 produced by effector T cells.
#1John A. Reynolds (University of Manchester)H-Index: 13
#2David W. RayH-Index: 68
Last. M. Yvonne AlexanderH-Index: 20
view all 6 authors...
Cardiovascular disease (CVD) is an important cause of morbidity and mortality in patients with systemic lupus erythematosus. The etiopathogenesis of premature CVD is not fully understood, but recently interferon-alpha (IFNα) has been implicated as a contributing factor. Since IFNα has been associated with both disease activity and endothelial dysfunction in lupus patients, we aimed to determine whether IFNα has direct effects on human aortic endothelial cell (HAoEC) function in vitro. We studied...
#1Naoki Takemura (UTokyo: University of Tokyo)H-Index: 20
#2Takumi Kawasaki (Nara Institute of Science and Technology)H-Index: 18
Last. Satoshi UematsuH-Index: 88
view all 22 authors...
Ionizing radiation damages small intestinal crypt cells, including epithelial stem cells and their progeny. Here the authors show that radiation-induced crypt cell death is amplified by the release of cellular RNA from apoptotic epithelial cells, which then triggers pro-apoptotic TLR3 signalling on neighbouring cells.
#1Yoshiaki Tabuchi (University of Toyama)H-Index: 30
#2Yukihiro FurusawaH-Index: 22
Last. Takashi Kondo (University of Toyama)H-Index: 57
view all 6 authors...
Purpose: Heat stress induces complex cellular responses, and its detailed molecular mechanisms still remain to be clarified. The objective of this study was to investigate the molecular mechanisms underlying cellular responses to mild hyperthermia (MHT) in normal human fibroblastic (NHF) cells.Materials and methods: Cells were treated with MHT (41°C, 30 min) and then cultured at 37°C. Gene expression was determined by the GeneChip® system and bioinformatics tools.Results: Treatment of the NHF ce...
#1Helen M. Lazear (WashU: Washington University in St. Louis)H-Index: 28
#2Alissa M. Lancaster (OHSU: Oregon Health & Science University)H-Index: 3
Last. Michael S. Diamond (WashU: Washington University in St. Louis)H-Index: 135
view all 15 authors...
Although the transcription factors IRF-3 and IRF-7 are considered master regulators of type I interferon (IFN) induction and IFN stimulated gene (ISG) expression, Irf3−/−×Irf7−/− double knockout (DKO) myeloid dendritic cells (mDC) produce relatively normal levels of IFN-β after viral infection. We generated Irf3−/−×Irf5−/−×Irf7−/− triple knockout (TKO) mice to test whether IRF-5 was the source of the residual induction of IFN-β and ISGs in mDCs. In pathogenesis studies with two unrelated positiv...
#1Ye Yuan (OU: Ohio University)H-Index: 7
#2Shin Hee Lee (OU: Ohio University)H-Index: 5
Last. Shiyong Wu (OU: Ohio University)H-Index: 29
view all 3 authors...
Alteration of adhesion molecule expression on endothelial cells has a direct connection with ionizing radiation-induced atherosclerosis, which is an adverse effect observed after radiotherapy. However, minimal attention has been given to monocytes/macrophages role in atherosclerosis development, which are exposed to the radiation at the same time. Under flow conditions using a parallel plate flow chamber to mimic physiological shear stress, we demonstrate here that the avidity between very late ...
#1Yukihiro Furusawa (University of Toyama)H-Index: 22
#2Zheng Li Wei (University of Toyama)H-Index: 3
Last. Takashi Kondo (University of Toyama)H-Index: 57
view all 9 authors...
Transforming growth factor-β-activated kinase 1 (TAK1) appears to play a role in inhibiting apoptotic death in response to multiple stresses. To assess the role of TAK1 in X-ray induced apoptosis and cell death, we irradiated parental and siRNA-TAK1-knockdown HeLa cells. Changes in gene expression levels with and without TAK1-knockdown were also examined after irradiation to elucidate the molecular mechanisms involved. After X-ray irradiation, cell death estimated by the colony formation assay i...
Cited By4
#1Yulin Zhang (CQMU: Chongqing Medical University)H-Index: 3
#2Xiaolan Ouyang (CQMU: Chongqing Medical University)H-Index: 2
Last. Lina Hu (CQMU: Chongqing Medical University)H-Index: 2
view all 8 authors...
Human amniotic epithelial cells (hAECs) show similar features to stem cells and have low immunogenicity. This study aims to investigate the therapeutic effect of hAEC transplantation on cyclophosphamide-induced primary ovarian insufficiency (POI) rats and evaluate the underlying mechanisms by mRNA sequencing of ovarian samples. Notably, hAECs mainly located in the interstitial area of the ovaries rather than follicles. hAEC transplantation led to a slight increase in body and ovary weight, norma...
#1Yukihiro Furusawa (University of Toyama)H-Index: 22
#2Yuka Yamanouchi (Toyama Prefectural University)H-Index: 1
Last. Takashi Kondo (University of Toyama)H-Index: 57
view all 9 authors...
Hyperthermia induced by heat stress (HS) is known to inhibit proliferation and induce cell death in cancer. We previously demonstrated that checkpoint kinase 1 (Chk1) contributes to G2/M arrest and cell survival under HS; however, the role of Chk2, a functional analog of Chk1, in regulation of the cell cycle and cell death under HS is still unknown. Here, we addressed the role of Chk2 using Molt-4 cells with p53-targeted shRNA (Molt-4/shp53) and parental control cells (Molt-4/V). Chk2 inhibition...
#1Jos PhilippH-Index: 4
#2Omid AzimzadehH-Index: 23
Last. Soile TapioH-Index: 31
view all 12 authors...
Radiation is the most common treatment of cancer. Minimizing the normal tissue injury, especially the damage to vascular endothelium, remains a challenge. This study aimed to analyze direct and indirect radiation effects on the endothelium by investigating mechanisms of signal transfer from irradiated to nonirradiated endothelial cells by means of secreted proteins. Human coronary artery endothelial cells (HCECest2) undergo radiation-induced senescence in vitro 14 days after exposure to 10 Gy X-...
#1Yaxing Zhang (WHU: Wuhan University)H-Index: 1
#2Hongliang Li (WHU: Wuhan University)H-Index: 1
Interferon regulatory factors (IRFs) are evolutionarily conserved proteins expressed not only in immune cells but also in other tissues and organs outside the immune system. In this review, we discuss mechanisms responsible for IRF-mediated innate immune responses and the function and mechanism of IRFs in cardiometabolic diseases. We focus on the role of IRFs in innate immunity and cardiometabolic homeostasis, and highlight reprogrammed IRF signaling.
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.