Isolation and Characterization of New Polymorphic Simple Sequence Repeat Loci in Chrysopogon aciculatus (Retz.) Trin.

Chrysopogon aciculatus (Retz.) Trin. is a perennial turfgrass for its low management and resistance. To develop simple sequence repeat (SSR) markers for C. aciculatus , we used four Roche 454 pyrosequencing, combined with the magnetic bead enrichment method FIASCO (fast isolation by amplified fragment length polymorphism of sequences containing repeats) to isolate from the C. aciculatus . A total of 66,198 raw sequencing reads were obtained with...