Optimisation of Chromatographic Resolution Using Objective Functions Including Both Time and Spectral Information. Part 2: Compounds Exhibiting Small Spectral Differences

Background: Current liquid chromatography instruments may yield two-way signals with full spectra collected as a function of time. These signals can be processed to recover chromatographic peaks in case of low resolution in the time order without any peak shape assumption. In Part I of this work, a two-way chromatographic objective function (COF) that incorporates both time and spectral information using diode array detection, based on the...