Abstract 2215: Use of peptide nucleic acid(PNA)-mediated PCR clamping method for detection EGFR mutation in patients with non-small cell lung cancer

Introduction : Somatic mutations in the epidermal growth factor receptor (EGFR) kinase domain are associated with sensitivity to EGFR-tyrosine kinase inhibitors (EGFR-TKIs) in patients with nonsmall cell lung cancer (NSCLC). Direct sequencing is known to be the standard for detecting EGFR mutations; however, it has limited sensitivity. Peptide nucleic acids (PNA)-mediated Real-Time PCR clamping method is a newly introduced method for analyzing...