Effect of Macrophages on Fibroblast DNA Synthesis and Proliferation

Published on Apr 1, 1982in Experimental Biology and Medicine3.139
· DOI :10.3181/00379727-169-41373
Robert F. Diegelmann63
Estimated H-index: 63
(MCV: VCU Medical Center),
Cohen Ik5
Estimated H-index: 5
(MCV: VCU Medical Center),
Alan M. Kaplan12
Estimated H-index: 12
(MCV: VCU Medical Center)
AbstractMouse peritoneal macrophages incubated with mouse 3T3 fibroblasts modulate DNA synthesis by the fibroblasts as measured by 125IUDR incorporation into DNA. The factor responsible for this activity is soluble and inhibits fibroblast DNA synthesis when present in high concentrations, whereas stimulation was observed at lower concentrations. Similar findings were observed when soluble fractions from human peripheral monocytes were incubated with primary human skin fibroblasts. In addition to increased DNA synthesis, there was a significant increase in human fibroblast proliferation as measured by direct cell counting. These findings support the hypothesis that macrophages play a regulatory role in wound healing through their capacity for positive and negative regulation of fibroblast DNA synthesis and proliferation.
📖 Papers frequently viewed together
1,177 Citations
7 Citations
81 Citations
Cited By19
Influenza virus infection of the lung has been shown, in animal models as well as in isolated human case reports, to cause a long-term alveolitis characterized by increased numbers of free lung macrophages. Recruitment of peripheral blood monocytes into the interstitial spaces, with subsequent maturation into tissue macrophages, accounts for most of the observed increase in cell numbers. The current studies examined the release of soluble factors by lavage-derived alveolar macrophages from healt...
#1B. Handan Ozdemir (Başkent University)H-Index: 14
#2F. Nurhan Ozdemir (Başkent University)H-Index: 14
Last. Mehmet Haberal (Başkent University)H-Index: 60
view all 4 authors...
Abstract The aim of this study was to clarify the relationship between angiogenesis and mononuclear cell infiltration in renal allografts. Kidney biopsies from 70 renal transplant recipients were evaluated. The density of CD68, CD3, and HLA-DR-positive infiltrating cells were semiquantitatively assessed by immunohistochemistry. Microvessels were highlighted by immunostaining endothelial cells for factor VIII-RA. Of the 70 patients, 38 showed acute rejection (AR), and 32 showed chronic rejection ...
29 CitationsSource
#1George J. Kahaly (University of Mainz)H-Index: 64
#2Cordula M. StoverH-Index: 25
Last. M. SchulerH-Index: 3
view all 5 authors...
Glycosaminoglycan (GAG) accumulation in the retrobulbar space of patients with thyroid-associated ophthalmopathy (TAO) has been documented in a number of immunohistochemical studies. In order to gain further insight into possible immunopathogenic mechanisms, the influence of humoral immunity on retrobulbar fibroblasts (RF) as GAG producing cells as well as on GAGs themselves was investigated. The effect of lymphocytes on hyaluronic acid (HA) synthesis of RF as well as in turn the influence of RF...
22 CitationsSource
#1Tracey L. Bonfield (Case Western Reserve University)H-Index: 34
#2Erica Colton (Case Western Reserve University)H-Index: 25
Last. James M. Anderson (Case Western Reserve University)H-Index: 106
view all 3 authors...
The studies presented in this paper evaluate the modulatory role of protein preadsorbed polydimethylsiloxane (PDMS) and Biomer on the secretion of fibroblast stimulating growth factors from human monocytes/macrophages. The results of these studies show that Biomer and PDMS selectively activate human monocytes to produce fibroblast “progression-like” and to a lesser extent “competence-like” stimulating growth factors. Polydimethylsiloxane stimulated the monocytes/macrophages to produce more “prog...
29 CitationsSource
#1S.R. Young (Guy's Hospital)H-Index: 2
#2Mary Dyson (Guy's Hospital)H-Index: 28
Abstract Macrophages area source of many important growth factors which can act as wound mediators during tissue repair. The aim of this work was to find out if levels of ultrasound which accelerate repair could stimulate the release of fibroblast mitogenic factors from an established macrophage-like cell line (U937). The U937 cells were exposed in vitro to continuous ultrasound at a space average, temporal average intensity of 0.5 W/cm 2 at either 0.75 MHz or 3.0 MHz, for 5 min. The macrophage-...
107 CitationsSource
#1Barbara M. Schreiber (BU: Boston University)H-Index: 20
#2Bernice M. Martin (BU: Boston University)H-Index: 8
Last. Carl Franzblau (BU: Boston University)H-Index: 56
view all 4 authors...
Abstract This report describes the enhancement of growth potentiating activity produced by mononuclear phagocytes that were incubated with β-migrating very low density lipoproteins (β-VLDL). Conditioned media harvested from cultured human peripheral blood monocytes incubated in the presence or absence of the lipoprotein were evaluated for their ability to stimulate DNA synthesis ([ 3 H]thymidine incorporation) of sparsely seeded quiescent BHK-21 (BHK) cells as well as neonatal rat aortic smooth ...
17 CitationsSource
#1Juan A. Vergara (Duke University)H-Index: 6
#2Raymond-U (Duke University)H-Index: 1
Last. Lyn A. Thet (Duke University)H-Index: 10
view all 3 authors...
Abstract We used stereologic-morphometric techniques to obtain a detailed quantitative picture of the changes in lung ultrastructure of rats at 12 and 26 weeks after unilateral thoracic irradiation with 3000 cGy. At 12 weeks post-radiation, the total number type 1 epithelial cells, type 2 epithelial cells and capillary endothelial cells were decreased 50–70%, total type 1 epithelial and capillary surface areas were decreased 55–60%, and the total volume of intracapillary blood was decreased 75%....
41 CitationsSource
#1Z. Fotev (UWA: University of Western Australia)H-Index: 1
#2Darrel Whitaker (UWA: University of Western Australia)H-Index: 20
Last. J. M. Papadimitriou (UWA: University of Western Australia)H-Index: 1
view all 3 authors...
The role of macrophages in rat mesothelial healing was evaluated in vivo and in vitro. In animals whose circulating monocytes were severely depressed, the healing rate was retarded; however, this effect was partly reversed by the introduction of macrophages into the injured site, suggesting that macrophages are of fundamental importance in the healing of mesothelial wounds. Furthermore, it was found that wound exudates stimulated DNA synthesis in normal quiescent mesothelial cells and the compon...
44 CitationsSource
#1Stephen C. Benson (CSU: California State University)H-Index: 19
#2John C. Belton (CSU: California State University)H-Index: 4
Last. Larry G. Scheve (CSU: California State University)H-Index: 1
view all 3 authors...
Male Sprague-Dawley rats were subjected to a single, intratracheal instillation of 30 mg Min-U-Sil silica in sterile saline and were sacrificed 3, 7, or 14 days following instillation. Control animals were instilled with sterile saline only. Silica instillation produced an inflammatory reaction followed by histological changes characteristic of lung fibrosis. Thickened alveolar septa associated with inflammatory cells transforming into large multifocal fibrotic nodules were detected in silica-ex...
26 CitationsSource