ATP-induced photoreceptor death in a feline model of retinal degeneration.

Published on Dec 1, 2014in Investigative Ophthalmology & Visual Science3.47
· DOI :10.1167/IOVS.14-15732
Felix P. Aplin7
Estimated H-index: 7
(University of Melbourne),
Chi D Luu34
Estimated H-index: 34
(University of Melbourne)
+ 3 AuthorsErica L. Fletcher51
Estimated H-index: 51
(University of Melbourne)
Sources
Abstract
This work was supported by the Australian Research Council (ARC)through its Special Research Initiative (SRI) in Bionic Vision Science and Technology grant to Bionic Vision Australia (BVA) and by the NHMRC project grant (APP1021042;APP1061419) to ELF by Retina Australia. CERA receives Operational Infrastructure Support from the Victorian Government and is supported by NHMRC Centre for Clinical Research Excellence Award 529923.
📖 Papers frequently viewed together
27 Citations
13 Citations
67 Citations
References70
Newest
#1Kirstan A. Vessey (University of Melbourne)H-Index: 26
#2Ursula GreferathH-Index: 26
Last. Erica L. FletcherH-Index: 51
view all 8 authors...
Many common causes of blindness involve the death of retinal photoreceptors, followed by progressive inner retinal cell remodeling. For an inducible model of retinal degeneration to be useful, it must recapitulate these changes. Intravitreal administration of adenosine triphosphate (ATP) has recently been found to induce acute photoreceptor death. The aim of this study was to characterize the chronic effects of ATP on retinal integrity. Five-week-old, dark agouti rats were administered 50 mM ATP...
27 CitationsSource
#1David A. X. Nayagam (University of Melbourne)H-Index: 16
#2Richard A. Williams (University of Melbourne)H-Index: 19
Last. Robert K. Shepherd (University of Melbourne)H-Index: 66
view all 22 authors...
Purpose To assess the safety and efficacy of chronic electrical stimulation of the retina with a suprachoroidal visual prosthesis. Methods Seven normally-sighted feline subjects were implanted for 96–143 days with a suprachoroidal electrode array and six were chronically stimulated for 70–105 days at levels that activated the visual cortex. Charge balanced, biphasic, current pulses were delivered to platinum electrodes in a monopolar stimulation mode. Retinal integrity/function and the mechanica...
34 CitationsSource
#1Yixin Zhang (Chinese PLA General Hospital)H-Index: 3
#2Houbin Huang (Chinese PLA General Hospital)H-Index: 3
Last. Zihao Liu (Chinese PLA General Hospital)H-Index: 3
view all 9 authors...
In the present study, the changes in the retinal nerve fiber layer (RNFL) thickness associated with Leber’s hereditary optic neuropathy (LHON) were examined by Cirrus high definition-optical coherence tomography (OCT), and the correlation between the RNFL thickness and the best corrected visual acuity (BCVA) was evaluated. A cross-sectional study was performed. Sixty-eight eyes from patients with LHON and 30 eyes from healthy individuals were scanned. Affected eyes were divided into 5 groups acc...
18 CitationsSource
#1Diego García-Ayuso (University of Murcia)H-Index: 17
#2Arturo Ortín-Martínez (University of Murcia)H-Index: 17
Last. María Paz Villegas-Pérez (University of Murcia)H-Index: 45
view all 9 authors...
Supported by the Spanish Ministry of Economy and Competitiveness Instituto de Salud Carlos III-Fondo Europeo de Desarrollo Regional PI10/01496, PI10/00187, FIS PS0901854, SAF-2012-38328, BFU2012-36845, RD12/0034/0010, and RD12/0034/0014; Fundacion Seneca de la Region de Murcia 04446/GERM/07; and Organizacion Nacional de Ciegos de Espana.
35 CitationsSource
Jul 3, 2013 in EMBC (International Conference of the IEEE Engineering in Medicine and Biology Society)
#1Yasuo Terasawa (Vision Institute)H-Index: 11
#2Hiroyuki Tashiro (Kyushu University)H-Index: 11
Last. Motoki Ozawa (Vision Institute)H-Index: 11
view all 5 authors...
Confirming safety of chronic electrical stimulation is of prime importance for the practical use of visual prostheses. Here we applied electrical stimulation to eyes of freely-moving rabbits eight hour per day for one month. Examinations including fundus photo, optical coherence tomography (OCT), electrically evoked potentials (EEPs) were performed before and after one-month stimulation to detect tissue damage. No adverse effect caused by electrical stimulation was observed in electrophysiologic...
16 CitationsSource
#1Joel Villalobos (Bionics Institute)H-Index: 9
#2David A. X. Nayagam (Bionics Institute)H-Index: 16
Last. Chris E. WilliamsH-Index: 67
view all 12 authors...
JV and DAXN contributed equally to the work presented here and should therefore be regarded as equivalent authors.
44 CitationsSource
#1Andrew I. Jobling (University of Melbourne)H-Index: 21
#2Kirstan A. VesseyH-Index: 26
Last. Erica L. FletcherH-Index: 51
view all 5 authors...
PURPOSE: This work investigates a novel, naturally occurring mouse model of achromatopsia. The specific missense mutation within the Gnat2 gene was identified and the subsequent retinal phenotype characterized. METHODS: The Gnat2 sequence was amplified using PCR from BALB/c and Gnat2(c.518A>G) retinae and the product sequenced. Retinal function was assessed at 3, 6, 9, and 12 months using the electroretinogram. Transducin and opsin expression were assessed at 3 and 12 months using immunohistoche...
30 CitationsSource
#1Mandeep S. Singh (University of Oxford)H-Index: 21
#2Peter Charbel Issa (University of Oxford)H-Index: 44
Last. Robert E MacLaren (NIHR: National Institute for Health Research)H-Index: 61
view all 8 authors...
One strategy to restore vision in retinitis pigmentosa and age-related macular degeneration is cell replacement. Typically, patients lose vision when the outer retinal photoreceptor layer is lost, and so the therapeutic goal would be to restore vision at this stage of disease. It is not currently known if a degenerate retina lacking the outer nuclear layer of photoreceptor cells would allow the survival, maturation, and reconnection of replacement photoreceptors, as prior studies used hosts with...
168 CitationsSource
#1Shoji Notomi (Kyushu University)H-Index: 18
#2Toshio Hisatomi (Kyushu University)H-Index: 48
Last. Tatsuro Ishibashi (Kyushu University)H-Index: 77
view all 11 authors...
Photoreceptor degeneration is the most critical cause of visual impairment in age-related macular degeneration (AMD). In neovascular form of AMD, severe photoreceptor loss develops with subretinal hemorrhage due to choroidal neovascularization (CNV), growth of abnormal blood vessels from choroidal circulation. However, the detailed mechanisms of this process remain elusive. Here we demonstrate that neovascular AMD with subretinal hemorrhage accompanies a significant increase in extracellular ATP...
49 CitationsSource
#1Magdalene J. Seiler (UCI: University of California, Irvine)H-Index: 40
#2Robert B. Aramant (UCI: University of California, Irvine)H-Index: 35
Retinal diseases such as age-related macular degeneration (ARMD) and retinitis pigmentosa (RP) affect millions of people. Replacing lost cells with new cells that connect with the still functional part of the host retina might repair a degenerating retina and restore eyesight to an unknown extent. A unique model, subretinal transplantation of freshly dissected sheets of fetal-derived retinal progenitor cells, combined with its retinal pigment epithelium (RPE), has demonstrated successful results...
107 CitationsSource
Cited By24
Newest
#1Alice Brandli (University of Melbourne)H-Index: 9
#2Stefanie Dudczig (University of Melbourne)H-Index: 4
Last. Patricia R. Jusuf (University of Melbourne)H-Index: 16
view all 4 authors...
Abstract Retinal regeneration research offers hope to people affected by visual impairment due to disease and injury. Ongoing research has explored many avenues towards retinal regeneration, including those that utilizes implantation of devices, cells or targeted viral-mediated gene therapy. These results have so far been limited, as gene therapy only has applications for rare single-gene mutations and implantations are invasive and in the case of cell transplantation donor cells often fail to i...
Source
Purpose null Artificial intelligence (AI) techniques are increasingly being used to classify retinal diseases. In this study we investigated the ability of a convolutional neural network (CNN) in categorizing histological images into different classes of retinal degeneration. null Methods null Images were obtained from a chemically induced feline model of monocular retinal dystrophy and split into training and testing sets. The training set was graded for the level of retinal degeneration and us...
Source
#2Yong Tang (CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 17
Source
Photoreceptor death is an important contributor to irreversible vision loss worldwide. In this review, I outline our work examining the role that purines, such as adenosine triphosphate (ATP), have in normal retinal function and in retinal disease. Our work shows that the actions of ATP, mediated by P2X receptors, are expressed in various retinal layers including photoreceptor terminals, and when stimulated by excessive levels of ATP is associated with rapid death of neurons. Treatment with a co...
1 CitationsSource
#1Souhei Maruyama (University of Toyama)H-Index: 1
#2Shin Ichi Akanuma (University of Toyama)H-Index: 20
Last. Ken Ichi Hosoya (University of Toyama)H-Index: 61
view all 4 authors...
Retinal pigment epithelial (RPE) cells form the outer blood-retinal barrier (BRB) and regulate drug/compound exchange between the neural retina and blood in the fenestrated blood vessels of retinal choroid via membrane transporters. Recent studies have elucidated that RPE cells express hemichannels, which are opened by extracellular Ca2+ depletion and accept several drugs/compounds as a transporting substrate. The objective of this study was to elucidate the hemichannel-mediated compound transpo...
Source
#1Matias Pedro Medrano (CONICET: National Scientific and Technical Research Council)H-Index: 3
#2Antonella Pisera‐Fuster (CONICET: National Scientific and Technical Research Council)H-Index: 1
Last. Maria Paula Faillace (UBA: University of Buenos Aires)H-Index: 10
view all 4 authors...
Injured retinas in mammals do not regenerate and heal with loss of function. The adult retina of zebrafish self-repairs after damage by activating cell-intrinsic mechanisms, which are regulated by extrinsic signal interactions. Among relevant regulatory extrinsic systems, purinergic signaling regulates progenitor proliferation during retinogenesis and regeneration and glia proliferation in proliferative retinopathies. ATP-activated P2X7 (P2RX7) and adenosine (P1R) receptors are involved in the p...
2 CitationsSource
#1Nicolás Cuenca (University of Alicante)H-Index: 40
#2Isabel Ortuño-Lizarán (University of Alicante)H-Index: 9
view all 13 authors...
Abstract: Optical coherence tomography (OCT) and OCT angiography (OCTA) have been a technological breakthrough in the diagnosis, treatment, and follow-up of many retinal diseases, thanks to its resolution and its ability to inform of the retinal state in seconds, which gives relevant information about retinal degeneration. In this review, we present an immunohistochemical description of the human and mice retina and we correlate it with the OCT bands in health and pathological conditions. Here, ...
10 CitationsSource
#1Gwang Myeong Noh (Kosin University)H-Index: 2
#2Jae Wan LimH-Index: 2
Last. Sang Joon Lee (Kosin University)H-Index: 11
view all 5 authors...
PURPOSE: We investigated structural changes in the retina by using optical coherence tomography (OCT) in a feline model of retinal degeneration using iodoacetic acid (IAA). METHODS: We examined 22 eyes of 11 felines over 2 years of age. The felines had fasted for 12 hours and were intravenously injected with IAA 20 mg/kg of body weight. OCT (Spectralis OCT) was performed at the point where the ends of the retinal vessels collected in the lateral direction from the optic nerve head and area centr...
Source
#1Changzoo Kim (Kosin University)
#3Seung Uk Lee (Kosin University)H-Index: 6
Last. Sang Joon Lee (Kosin University)H-Index: 11
view all 4 authors...
Source
#1So Min Ahn (KU: Korea University)H-Index: 3
#1So Min Ahn (KU: Korea University)H-Index: 3
Last. Seong Woo Kim (KU: Korea University)H-Index: 19
view all 8 authors...
We sought to develop and characterize outer retinal degeneration induced by intravitreal injection of sodium iodate (SI) after vitrectomy in rabbits. To determine the effective dose of SI, the right eyes of 19 male New Zealand white rabbits received an intravitreal injection of SI or sham. Based on the dose-dependence results, 0.4 mg of SI in 0.05 mL of total volume was injected into the right eyes of 10 rabbits at two weeks after vitrectomy. In the dose-dependence study, localized retinal atrop...
5 CitationsSource