Missense mutations in the signal peptide of the porcine GH gene affect cellular synthesis and secretion

In previous investigations, we have demonstrated the mutations in the signal peptide of porcine GH gene were associated with the body size. In this study, the fusion gene expression vectors which consisted of eight signal peptide mutants of GH gene and EGFP gene were constructed according to three missense mutations (p.Val9Ala, p.Gln22Arg and p.Asp25Gly), and they were transfected into the GH3 cell line. The inhibition levels of EGFP gene...