Proliferation and migration of epithelial cells during corneal wound repair in the rabbit and the rat.

Published on Jan 1, 1966in American Journal of Ophthalmology5.258
· DOI :10.1016/0002-9394(66)90747-1
Calvin Hanna19
Estimated H-index: 19
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The left eyes of young and adult mice and rats were exposed to 2400 r of gamma radiation from a Co/sup 60/ source administered at the rate of 40 r/min. The right eyes of the animals served as controls. Thymidine--tritium is incorporated into the deoxyribonucleic acid (DNA) in the synthesis phase of cell division. The number of epithelial cells labeled per section of lens and the number of grains found per cell were taken as a measure of nucleic acid synthesis. The change in position of labeled c...
#1P. Block (University of Freiburg)H-Index: 1
#2I. Seiter (University of Freiburg)H-Index: 1
Last. W. Oehlert (University of Freiburg)H-Index: 5
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Abstract The autoradiographic method was employed to study the mechanism of the response to injury of the epithelium and connective tissue in the skin and tongue of the rat. Skin and tongue were studied at various times after an incision had been made in them, and after superficial cauterization of the tongue. 1. 1. The tongue showed a rapid increase in the number of labeled cells 200–300 basal cells distant from the wound edge. Very few cells were labeled immediately adjacent to the defect unti...
The aim of the corneal transplant in man is to maintain corneal transparency. Even under the best conditions a proportion of the grafts that initially "take" suffer from the clinical problem of "graft sickness" with late clouding. These grafts are the homoplastic type, and evidence is accumulating which indicates that the homograft rejection phenomenon in the cornea is an immune response. 1,2 Thus, the clouding in an otherwise successful graft would result from a reaction of the host to the anti...
Since the removal of only one of the two nucleic acids, ribonucleic and deoxyribonucleic, is often necessary in biological work, the optimum conditions for the specific removal of either nucleic acid by the corresponding enzyme were investigated. The specific removal of nucleic acids by enzyme treatment was tested by toluidine blue staining and by quantitative study of the radioautographic reactions produced by the labelled deoxyribonucleic acid and ribonucleic acid appearing in tissues of a cyt...
When a corneal transplant "takes," a basic problem remains as to which corneal elements are replaced following the graft. The fate of the sulfated mucopolysaccharides of the transplant has been studied by several investigators using S-35 1,2 but methods are needed to specifically label and locate individual cells in order to study the movement of the cellular elements. In recent years thymidine-tritium has been used in studying the synthesis of desoxyribonucleic acid (DNA). Once incorporated int...
#1Calvin HannaH-Index: 19
#2Donald S. BicknellH-Index: 1
Last. James E. O'brienH-Index: 5
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Previous reports from our laboratory 1-3 indicate that tritium-labeled thymidine can be used to study cell production and migration following intraocular injection in animals. A recent editorial 4 points out the usefulness of this method of nuclear labeling since the thymidine-tritium is incorporated into cellular desoxyribonucleic acid (DNA) during the premitotic coupling stage of cell division. This stage is normally followed by the remainder of cell division 5 (see text). The tritium tag is q...
The epithelial layer of the lens has been implicated in the pathogenesis of several types of cataract. 1 These types, which are progressive, slow to develop, and usually nonreversible, include various radiation cataracts such as those caused by ultraviolet, infrared, x-ray, γ-ray, neutron, β-ray, and radiomimetic agents such as busulfan (Myleran).* In a number of animal experiments where the germinative zone of the lens epithelium was selectively irradiated a slow forming opacity was found, wher...
#1Clifford V. Harding (Columbia University)H-Index: 8
#2A. Donn (Columbia University)H-Index: 1
Last. B. Dobli Srinivasan (Columbia University)H-Index: 4
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Cited By110
#1Mohd Tayyab Adil (UIUC: University of Illinois at Urbana–Champaign)H-Index: 2
#2Jonathan J. Henry (UIUC: University of Illinois at Urbana–Champaign)H-Index: 26
Animal models have contributed greatly to our understanding of human diseases. Here, we focus on cornea epithelial stem cell (CESC) deficiency (commonly called limbal stem cell deficiency, LSCD). Corneal development, homeostasis and wound healing are supported by specific stem cells, that include the CESCs. Damage to or loss of these cells results in blindness and other debilitating ocular conditions. Here we describe the contributions from several vertebrate models toward understanding CESCs an...
#1Koji Sugioka (Kindai University)H-Index: 11
#2Ken Fukuda (Kōchi University)H-Index: 28
Last. Shunji Kusaka (Kindai University)H-Index: 36
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Abstract The cornea is a relatively unique tissue in the body in that it possesses specific features such as a lack of blood vessels that contribute to its transparency. The cornea is supplied with soluble blood components such as albumin, globulin, and fibrinogen as well as with nutrients, oxygen, and bioactive substances by diffusion from aqueous humor and limbal vessels as well as a result of its exposure to tear fluid. The healthy cornea is largely devoid of cellular components of blood such...
#1Sudan Puri (UH: University of Houston)H-Index: 4
#2Mingxia Sun (UH: University of Houston)H-Index: 3
Last. Vivien J. Coulson-Thomas (UH: University of Houston)H-Index: 20
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Purpose Establishing the dynamics of corneal wound healing is of vital importance to better understand corneal inflammation, pathology, and corneal regeneration. Numerous studies have made great strides in investigating multiple aspects of corneal wound healing; however, some aspects remain to be elucidated. This study worked toward establishing (1) if epithelial limbal stem cells (LSCs) are necessary for healing all corneal wounds, (2) the mechanism by which epithelial cells migrate toward the ...
#1Natalia Murataeva (IU: Indiana University)H-Index: 9
#2Laura Daily (IU: Indiana University)H-Index: 6
Last. Alex Straiker (IU: Indiana University Bloomington)H-Index: 30
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PURPOSE: We previously showed that cannabinoid-related GPR18 receptors are present in the murine corneal epithelium, but their function remains unknown. The related CB1 receptors regulate corneal healing, possibly via chemotaxis. We therefore examined a potential role for GPR18 in corneal epithelial chemotaxis and wound healing. METHODS: We examined GPR18 messenger RNA (mRNA) and protein expression in the cornea. We additionally examined GPR18 action in cultured bovine corneal epithelial cells (...
#1Patricia Rousselle (University of Lyon)H-Index: 46
#2Fabienne Braye (University of Lyon)H-Index: 17
Last. Guila Dayan (University of Lyon)H-Index: 3
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Abstract Cutaneous wound healing in adult mammals is a complex multi-step process involving overlapping stages of blood clot formation, inflammation, re-epithelialization, granulation tissue formation, neovascularization, and remodelling. Re-epithelialization describes the resurfacing of a wound with new epithelium. The cellular and molecular processes involved in the initiation, maintenance, and completion of epithelialization are essential for successful wound closure. A variety of modulators ...
#1Mohammed Ziaei (University of Auckland)H-Index: 12
#2Carol Ann Greene (University of Auckland)H-Index: 5
Last. Colin R. Green (University of Auckland)H-Index: 36
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Abstract In order to maintain a smooth optical surface the corneal epithelium has to continuously renew itself so as to maintain its function as a barrier to fluctuating external surroundings and various environmental insults. After trauma, the cornea typically re-epithelializes promptly thereby minimizing the risk of infection, opacification or perforation. A persistent epithelial defect (PED) is usually referred to as a non-healing epithelial lesion after approximately two weeks of treatment w...
#1Farhan Taherali (UCL: University College London)H-Index: 5
#2Felipe J.O. VarumH-Index: 13
Last. Abdul Basit (UCL: University College London)H-Index: 84
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Abstract The mucosa of the gastrointestinal tract, eyes, nose, lungs, cervix and vagina is lined by epithelium interspersed with mucus-secreting goblet cells, all of which contribute to their unique functions. This mucus provides an integral defence to the epithelium against noxious agents and pathogens. However, it can equally act as a barrier to drugs and delivery systems targeting epithelial passive and active transport mechanisms. This review highlights the various mucins expressed at differ...
#1Alexander Richardson (UNSW: University of New South Wales)H-Index: 10
#2Denis Wakefield (UNSW: University of New South Wales)H-Index: 74
Last. Nick Di Girolamo (UNSW: University of New South Wales)H-Index: 32
view all 3 authors...
The anterior aspect of the cornea consists of a stratified squamous epithelium, thought to be maintained by a rare population of stem cells (SCs) that reside in the limbal transition zone. Although migration of cells that replenish the corneal epithelium has been studied for over a century, the process is still poorly understood and not well characterized. Numerous techniques have been employed to examine corneal epithelial dynamics, including visualization by light microscopy, the incorporation...
#1Nick Di Girolamo (UNSW: University of New South Wales)H-Index: 32
Abstract Lineage tracing allows the destiny of a stem cell (SC) and its progeny to be followed through time. In order to track their long-term fate, SC must be permanently marked to discern their distribution, division, displacement and differentiation. This information is essential for unravelling the mysteries that govern their replenishing activity while they remain anchored within their niche microenvironment. Modern-day lineage tracing uses inducible genetic recombination to illuminate cell...
#1N. Di Girolamo (UNSW: University of New South Wales)H-Index: 19
#2Samantha BobbaH-Index: 7
Last. James Guy Lyons (USYD: University of Sydney)H-Index: 2
view all 10 authors...
Stem cell (SC) division, deployment, and differentiation are processes that contribute to corneal epithelial renewal. Until now studying the destiny of these cells in a living mammal has not been possible. However, the advent of inducible multicolor genetic tagging and powerful imaging technologies has rendered this achievable in the translucent and readily accessible murine cornea. K14CreERT2-Confetti mice that harbor two copies of the Brainbow 2.1 cassette, yielding up to 10 colors from the st...
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