Different methods of detaching adherent cells significantly affect the detection of TRAIL receptors.
Abstract
As a powerful technique allowing analysis of large numbers of cells, fluorescence-activated cell sorting (FACS) is used more and more widely. For FACS analysis, adherent cells are usually detached by trypsinization, followed by centrifugation and resuspension. However, trypsinization can cut off some receptors from the cell surface like fine scissors, which will affect the accuracy of FACS results. Though non-enzymatic methods such as citric...
Paper Details
Title
Different methods of detaching adherent cells significantly affect the detection of TRAIL receptors.
Published Date
Nov 1, 2012
Journal
Volume
98
Issue
6
Pages
800 - 3
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