Retinoprotective Effects of Bilberry Anthocyanins via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms in a Visible Light-Induced Retinal Degeneration Model in Pigmented Rabbits

Published on Dec 14, 2015in Molecules3.267
· DOI :10.3390/MOLECULES201219785
Yong Wang11
Estimated H-index: 11
(CAU: China Agricultural University),
Yong Wang67
Estimated H-index: 67
(CAU: China Agricultural University)
+ 4 AuthorsFenghong Huang2
Estimated H-index: 2
(CRI: Crops Research Institute)
Excessive visible light exposure can induce damage to retinal cells and contribute to the development or progression of age-related macular degeneration. In this study we created a model of phototoxicity in pigmented rabbits. Furthermore, we investigated the protective effect of bilberry anthocyanin extract (BAE, Table A1) and explored the possible mechanisms of action in this model. The model of light-induced retinal damage was established by the pigmented rabbits exposed to light at 18,000 lx for 2 h, and they were sacrificed on day 7. After administration of BAE at dosages of 250 and 500 mg/kg/day, retinal dysfunction was significantly inhibited in terms of electroretinograms, and the decreased thicknesses of retinal outer nuclear layer and lengths of the outer segments of the photoreceptor cells were suppressed in rabbits with retinal degeneration. BAE attenuated the changes caused by light to certain apoptotic proteins (Bax, Bcl-2, and caspase-3). The extract increased the levels of superoxide dismutase, glutathione peroxidase, and catalase, as well as the total antioxidant capacity, but decreased the malondialdehyde level in the retinal cells. BAE inhibited the light-induced elevation in the levels of proinflammatory cytokines and angiogenic parameters (IL-1β and VEGF). Results showed that visible light-induced retinal degeneration model in pigmented rabbits was successfully established and BAE exhibited protective effects by increasing the antioxidant defense mechanisms, suppressing lipid peroxidation and proinflammatory cytokines, and inhibiting retinal cells apoptosis.
📖 Papers frequently viewed together
32 Citations
11 Citations
206 Citations
#1Daniela Calzia (UniGe: University of Genoa)H-Index: 20
#2Michele Oneto (UniGe: University of Genoa)H-Index: 10
Last. Isabella Panfoli (UniGe: University of Genoa)H-Index: 29
view all 11 authors...
Background and Purpose The rod outer segments (OS) of the retina are specialized organelles where phototransduction takes place. The mitochondrial electron transport complexes I–IV, cytochrome c and FoF1-ATP synthase are functionally expressed in the OS disks. Here, we have studied the effect of some polyphenolic compounds acting as inhibitors of mitochondrial ATPase/synthase activity on the OS ectopic FoF1- ATP synthase. The mechanism of apoptosis in the OS was also investigated studying the ex...
21 CitationsSource
#1Laura Fernández-Sánchez (University of Alicante)H-Index: 20
#2Pedro Lax (University of Alicante)H-Index: 23
Last. Nicolás Cuenca (University of Alicante)H-Index: 40
view all 6 authors...
All retinal disorders, regardless of their aetiology, involve the activation of oxidative stress and apoptosis pathways. The administration of neuroprotective factors is crucial in all phases of the pathology, even when vision has been completely lost. The retina is one of the most susceptible tissues to reactive oxygen species damage. On the other hand, proper development and functioning of the retina requires a precise balance between the processes of proliferation, differentiation and program...
24 CitationsSource
#1Yong Wang (CAU: China Agricultural University)H-Index: 11
#1Yong Wang (CAU: China Agricultural University)H-Index: 67
Last. Baoping Ji (CAU: China Agricultural University)H-Index: 32
view all 6 authors...
BACKGROUND Studies have shown that anthocyanins (ACNs) in berries contribute to eye health. However, information on the relationship between the chemical structures and visual functions of ACNs is scarce. This study investigated the protection effects of ACNs with different structures against visible light-induced damage in human retinal pigment epithelial (RPE) cells. RESULTS Four ACNs with different aglycones, namely, pelargonidin-3-glucoside (Pg-3-glu), cyanidin-3-glucoside (Cy-3-glu), delphi...
32 CitationsSource
#1Hak Sung Lee (SNU: Seoul National University)H-Index: 5
#2Jae-Hyun JunH-Index: 1
Last. Yeong Shik Kim (SNU: Seoul National University)H-Index: 79
view all 5 authors...
Epigalloccatechin-3-gallate (EGCG) is the main polyphenol component of green tea (leaves of Camellia sinensis). EGCG is known for its antioxidant, anti-inflammatory, antiviral, and anti-carcinogenic properties. Here, we identify EGCG as a new inhibitor of ocular angiogenesis and its vascular permeability. Matrix metalloproteinases (MMPs) and vascular endothelial growth factor (VEGF) play a key role in the processes of extracellular matrix (ECM) remodeling and microvascular permeability during an...
51 CitationsSource
#1Toshio Narimatsu (Keio: Keio University)H-Index: 6
#2Yoko Ozawa (Keio: Keio University)H-Index: 44
Last. Kazuo Tsubota (Keio: Keio University)H-Index: 120
view all 7 authors...
Background To elucidate the biological effects of blocking fluorescent light on the retina using specific blocking materials. Methods Seven- to 8-week-old BALB/c mice were divided into three groups and placed in one of the three boxes: one blocked ultraviolet and violet wavelengths of light (violet blockade), one blocked ultraviolet, violet, blue and some other visible wavelengths (blue-plus blockade), and one allowed most visible light to pass through (control). They were then exposed to a whit...
26 CitationsSource
#1Tomohiro Otsuka (Gifu Pharmaceutical University)H-Index: 6
#2Masamitsu Shimazawa (Gifu Pharmaceutical University)H-Index: 60
Last. Hideaki Hara (Gifu Pharmaceutical University)H-Index: 78
view all 8 authors...
Dietary carotenoids exhibit various biological activities, including antioxidative activity. In particular, astaxanthin, a type of carotenoid, is well known as a powerful antioxidant. We investigated whether astaxanthin would protect against light-induced retinal damage. In an in vivo study, ddY male mice were exposed to white light at 8,000 lux for 3 h to induce retinal damage. Five days after light exposure, retinal damage was evaluated by measuring electroretino- gram (ERG) amplitude and oute...
33 CitationsSource
#1Cora Roehlecke (TUD: Dresden University of Technology)H-Index: 11
#2Ulrike Schumann (TUD: Dresden University of Technology)H-Index: 4
Last. Richard Funk (TUD: Dresden University of Technology)H-Index: 38
view all 7 authors...
The retina is prone to oxidative stress from many factors which are also involved in the pathogenesis of degenerative diseases. In this study, we used the application of blue light as a physiological stress factor. The aim of this study was to identify the major source of intracellular ROS that mediates blue light-induced detrimental effects on cells which may lead to cytotoxicity. We hypothesized that outer segments are the major source of blue light induced ROS generation. In photoreceptors, n...
58 CitationsSource
#1Daniela Calzia (UniGe: University of Genoa)H-Index: 20
#2Stefano Barabino (UniGe: University of Genoa)H-Index: 27
Last. Isabella Panfoli (UniGe: University of Genoa)H-Index: 29
view all 14 authors...
Background Information. The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I–IV and F1Fo-ATP synthase in OS disks, as well as active soluble tricarboxylic acid cycle enzymes. Here, we focussed our study on the whole OS that contains the cytosol and plasma membrane and disks as native flattened saccules, unlike spherica...
22 CitationsSource
#1Hema L. Ramkumar (UCSD: University of California, San Diego)H-Index: 11
#2Jingsheng TuoH-Index: 34
Last. Chi-Chao ChanH-Index: 104
view all 7 authors...
The Age-Related Eye Diseases Study 2 (AREDS2) clinical trial is assessing the effects of higher dietary xanthophyll (lutein and zeaxanthin) and long-chain n3 polyunsaturated fatty acid (LCPUFA) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) intake on progression to advanced age-related macular degeneration (AMD). This study’s purpose was to examine the retinal effects of the AREDS2 formulation on Chemokine (C-C motif) ligand 2 (Ccl2−/−)/CX3C chemokine receptor 1 (Cx3cr1−/−) mice on C...
40 CitationsSource
#1Suresh Kumar Gupta (Delhi Institute of Pharmaceutical Sciences and Research)H-Index: 37
#2Binit KumarH-Index: 14
Last. Ashutosh AggarwalH-Index: 1
view all 7 authors...
Abstract Purpose: The present study was aimed to evaluate the retinoprotective effects of Moringa oleifera (MO) in Streptozotocin-induced diabetic rats. Methods: The study was continued for 24 weeks and evaluated for inflammatory (tumor necrosis factor [TNF]-α and interleukin [IL]-1β, angiogenic (vascular endothelial growth factor [VEGF] and protein kinase C [PKC]-β) and antioxidant (Glutathione, Superoxide dismutase, and Catalase) parameters. Retinal leakage was checked by Fluorescein angiograp...
35 CitationsSource
Cited By27
Anthocyanins are natural products that give color to plants. As natural plant pigments, anthocyanins also have a series of health-promoting benefits. Many researchers have proved that anthocyanins have therapeutic effects on diseases, such as circulatory, nervous, endocrine, digestive, sensory, urinary and immune systems. Additionally, a large number of studies have reported that anthocyanins have an anticancer effect through a wide range of anti-inflammatory and antioxidant effects. The anti-di...
#1Masumi G Asahi (GW: George Washington University)H-Index: 3
#2Jaycob Avaylon (California Northstate University College of Pharmacy)H-Index: 1
Last. Ron P. Gallemore (UCLA: University of California, Los Angeles)H-Index: 3
view all 4 authors...
Introduction Age-related macular degeneration (AMD) is the leading cause of blindness in individuals over age 50 in developed countries. Current therapy for nonexudative AMD (neAMD) is aimed at modifying risk factors and vitamin supplementation to slow progression while intravitreal anti-vascular endothelial factor (VEGF) injections are the mainstay for treatment of choroidal neovascularization in exudative AMD (eAMD). Areas covered Over the past decade, promising therapies have emerged that aim...
Retinal degenerations comprise a large heterogeneous class of diseases (dystrophies) of retina leading to a progressive vision loss. The emergence of new experimental therapies (gene and cell therapy, regenerative ophthalmic surgery) generates a demand for new experimental models. The review tackles various animal models of human retinal degenerative diseases. Pros and contras of induced and genetic experimental models are highlighted. An adequate experimental model choice may condition a pathog...
#1Hareth Y. ShujaaEdin (UPM: Universiti Putra Malaysia)H-Index: 1
#1Hareth Y. Shujaa Edin (UPM: Universiti Putra Malaysia)
view all 8 authors...
Abstract Recombinant human erythropoietin (rHuEPO) is the erythropoiesis-stimulating hormone that is being used concurrently with chemotherapeutic drugs in the treatment of anemia of cancer. The effect of rHuEPO on cancer cells in 3-dimensional (3D) cultures is not known. The objective of the study was to determine the effect of recombinant human erythropoietin (rHuEPO) on the viability of MCF-7 breast cancer cells from 2-dimensional (2D) and 3D cell cultures. The monolayer MCF-7 cells from 2D c...
#1Jessica K. W. Tsang (Li Ka Shing Faculty of Medicine, University of Hong Kong)H-Index: 1
#2Susanne A. Wolf (Charité)H-Index: 37
Last. Amy C. Y. Lo (Li Ka Shing Faculty of Medicine, University of Hong Kong)H-Index: 35
view all 7 authors...
Retinopathy of prematurity (ROP), the most common cause of childhood blindness, is a hypoxia-induced eye disease characterized by retinal neovascularization. In the normal retina, a well-organized vascular network provides oxygen and nutrients as energy sources to maintain a normal visual function; however, it is disrupted when pathological angiogenesis is induced in ROP patients. Under hypoxia, inadequate oxygen and energy supply lead to oxidative stress and stimulate neovasculature formation a...
#1Neeru BhattH-Index: 2
#2Mostafa I. WalyH-Index: 26
Last. Amanat AliH-Index: 18
view all 3 authors...
#1Joana Henriques (UC: University of Coimbra)H-Index: 4
#2Diana Serra (UC: University of Coimbra)H-Index: 8
Last. Leonor M. Almeida (UC: University of Coimbra)H-Index: 35
view all 4 authors...
Anthocyanins are naturally occurring polyphenols commonly found in fruits and vegetables. Numerous studies have described that anthocyanin-rich foods may play a crucial role in the prevention and treatment of different pathological conditions, which have encouraged their consumption around the world. Anthocyanins exhibit a significant neuroprotective role, mainly due to their well-recognized antioxidant and anti-inflammatory properties. Neuroinflammation is an intricate process relevant in both ...
3 CitationsSource
#1V. I. DeinekaH-Index: 3
#1Victor I. DeinekaH-Index: 8
Last. L. A. DeinekaH-Index: 6
view all 5 authors...
#1Jiao Yang (PKU: Peking University)H-Index: 1
#2Dankang Li (Zhengzhou University)H-Index: 1
Last. Zhaofeng Zhang (PKU: Peking University)H-Index: 27
view all 10 authors...
Abstract The present study aimed to evaluate the effects of lutein on blue LED light-induced retinal injury. Animals were randomly assigned to six groups. Distilled water (normal control and water-light group), corn oil (solvent-light group), or 25, 50, or 100 mg/kg lutein in corn oil (three lutein treatment groups) were administered intragastrically for 30 days. The experimental rats (all groups other than the normal control group) were exposed to 1500 lx for 1.5 h after pretreatment. Lutein pr...
1 CitationsSource
#1M. BottoniH-Index: 5
#2F. MilaniH-Index: 1
Last. Gelsomina FicoH-Index: 22
view all 8 authors...
This ethnobotanical survey was carried out in Caspoggio (Valmalenco, SO, Italy) with the purpose of investigating the traditional uses of medicinal plants. Moreover, a bibliographic research meant to validate or refute the uses, focusing on the potentially responsible compounds, was performed. Fifty-nine species, attributable to 30 families (Asteraceae, Pinaceae, Malvaceae, and Lamiaceae the most cited), were mentioned. Arnica montana, anti-inflammatory for traumas and musculoskeletal pains; Pin...
1 CitationsSource