Adipose-Derived Stem Cells: Origin and Lineage

Published on Jan 1, 2011
KG Marra2
Estimated H-index: 2
Kacey G. Marra64
Estimated H-index: 64
+ 0 AuthorsJ. Peter Rubin61
Estimated H-index: 61
(University of Pittsburgh)
Background: The potential of using mesenchymal stem cells derived from adipose tissue to enhance wound healing is significant. Considerable advances in both basic science and clinical research are being attained. The Problem: Nonhealing wounds remain a significant challenge for plastic surgeons. More than 600,000 people suffer from venous ulcers and 1.5‐3 million people are being treated for pressure sores every year in the United States. Basic/Clinical Science Advances: The use of tissue engineering techniques such as stem cell therapy and gene therapy to improve wound healing is a promising strategy. Adipose tissue represents a source of cells that may be able to enhance wound healing. Adipose-derived stem cells (ASCs) are adult mesenchymal stem cells that are easily harvested and of great interest in regenerative medicine applications. Specifically, ASCs secrete angiogenic growth factors and cytokines that can induce and enhance wound healing. Clinical Care Relevance: The improvement of angiogenesis, and hence vascularization, to the wound site will have a profound impact on the regeneration of tissue in impaired wound healing sites. Conclusions: This chapter describes innovative research strategies using ASC therapies for treatment of chronic, nonhealing wounds. These therapies include direct application of ASCs to wounds as well as the combination of ASCs with biomaterials.
📖 Papers frequently viewed together
91 Citations
42 Citations
81 Citations
#1Chunlei Nie (HMU: Harbin Medical University)H-Index: 6
#2Daping Yang (HMU: Harbin Medical University)H-Index: 11
Last. Steven F. Morris (Dal: Dalhousie University)H-Index: 14
view all 3 authors...
Wounds, characterized by leading to a loss of integrity of the skin and a major cause of morbidity and mortality, are common challenges encountered in plastic and reconstructive surgery. The primary goals of treatment are rapid closure, restoration of function, and aesthetical satisfaction. Adult stem cells may provide new strategies to treat cutaneous wounds because of their prolonged self-renewal capacity and ability to differentiate into various tissues. In the past five years, some researche...
91 CitationsSource
#1Teni Ebrahimian (Institut de radioprotection et de sûreté nucléaire)H-Index: 9
#2F. PouzouletH-Index: 1
Last. Radia TamaratH-Index: 22
view all 10 authors...
Objective— We hypothesized that adipose tissue may contain progenitors cells with cutaneous and angiogenic potential. Methods and Results— Adipose tissue-derived stroma cells (ADSCs) were administrated to skin punched wounds of both nonirradiated and irradiated mice (20 Gy, locally). At day14, ADSCs promoted dermal wound healing and enhanced wound closure, viscolesticity, and collagen tissue secretion in both irradiated and nonirradiated mice. Interestingly, GFP-positive ADSCs incorporated in de...
304 CitationsSource
#1Ludwik K. Branski (Shriners Hospitals for Children - Galveston)H-Index: 25
#2Gerd G. Gauglitz (UTMB: University of Texas Medical Branch)H-Index: 8
Last. Marc G. Jeschke (UTMB: University of Texas Medical Branch)H-Index: 74
view all 4 authors...
Different therapies that effect wound repair have been proposed over the last few decades. This article reviews the emerging fields of gene and stem cell therapy in wound healing. Gene therapy, initially developed for treatment of congenital defects, is a new option for enhancing wound repair. In order to accelerate wound closure, genes encoding for growth factors or cytokines showed the greatest potential. The majority of gene delivery systems are based on viral transfection, naked DNA applicat...
313 CitationsSource
#1Masaki Nambu (National Defense Medical College)H-Index: 10
#2Satoko KishimotoH-Index: 10
Last. Yasuhiro KanataniH-Index: 24
view all 11 authors...
Abstract:Adipose tissue-derived stromal cells (ATSCs) have recently gained widespread attention as a potential alternate source to bone marrow–derived mesenchymal stem cells with a proliferative capacity and a similar ability to undergo multilineage differentiation. In this study, we evaluated the e
220 CitationsSource
#1Matthew W. Blanton (IU: Indiana University)H-Index: 4
#2Ivan Hadad (IUPUI: Indiana University – Purdue University Indianapolis)H-Index: 5
Last. Keith L. MarchH-Index: 61
view all 7 authors...
Background:The authors examined the efficacy of adipose stem cells, when supplied either alone or in platelet-rich fibrin gels, to improve wound healing.Methods:A porcine full-thickness wound model was used to compare six topical treatments: platelet-poor plasma; platelet-rich plasma; autologous adi
159 CitationsSource
#1Mihaela Crisan (University of Pittsburgh)H-Index: 26
#2Solomon Yap (University of Pittsburgh)H-Index: 5
Last. Bruno Péault (University of Pittsburgh)H-Index: 72
view all 21 authors...
Summary Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rβ expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or...
3,558 CitationsSource
#1Bret M. Schipper (University of Pittsburgh)H-Index: 3
#2Kacey G. MarraH-Index: 64
Last. J. Peter RubinH-Index: 61
view all 5 authors...
Adipose tissue has been shown to contain adult mesenchymal stem cells that have therapeutic applications in regenerative medicine. There is evidence that the ability of adipose precursor cells to grow and differentiate varies among fat depots and changes with age. Defining these variations in cell function and molecular mechanisms of adipogenesis will facilitate the development of cell-based therapies. We compared cells harvested from 5 different subcutaneous (SC) adipose depots in 12 female pat...
351 CitationsSource
#1Dmitry O. Traktuev (IU: Indiana University)H-Index: 17
#2Stephanie Merfeld-Clauss (IU: Indiana University)H-Index: 11
Last. Keith L. March (IU: Indiana University)H-Index: 61
view all 8 authors...
It has been shown that stromal–vascular fraction isolated from adipose tissues contains an abundance of CD34+ cells. Histological analysis of adipose tissue revealed that CD34+ cells are widely distributed among adipocytes and are predominantly associated with vascular structures. The majority of CD34+ cells from freshly isolated stromal–vascular fraction were CD31−/CD144− and could be separated from a distinct population of CD34+/CD31+/CD144+ (endothelial) cells by differential attachment on un...
994 CitationsSource
#1Won-Serk Kim (SKKU: Sungkyunkwan University)H-Index: 16
#1Won Serk KimH-Index: 17
Last. Jeong-Soo Park (DU: Dankook University)H-Index: 16
view all 7 authors...
Summary Background Adipose-derived stem cells (ADSCs) are a population of pluripotent cells, which have characteristics similar to bone marrow-derived mesenchymal stem cells. Whereas ADSCs have potential applications for the repair and regeneration of various damaged tissues, few studies have dealt with the effect of ADSCs on fibroblasts, which play a key role in skin biology. Objective In this study, we investigated the possible roles of ADSCs in skin wound healing process, especially in the as...
917 CitationsSource
#1Marc G. Jeschke (UTMB: University of Texas Medical Branch)H-Index: 74
#2David N. Herndon (Shriners Hospitals for Children)H-Index: 128
Insulin-like growth factor-I (IGF-I) and keratinocyte growth factor (KGF) cDNA gene transfer individually improves dermal and epidermal regeneration. The aim of the present study was to determine whether the combination of IGF-I plus KGF cDNA further improves wound healing and by which mechanisms these changes occur. Rats received an acute wound and were divided into four groups to receive weekly subcutaneous injections of liposomes plus Lac Z cDNA, liposomes plus IGF-I cDNA, liposomes plus KGF ...
22 CitationsSource
Cited By0