FABRICATION AND CHARACTERIZATION OF ELECTROSPUN CHITOSAN/POLY(L-LACTIC ACID) NANO/MICRO FIBERS AS A SCAFFOLD FOR BONE REGENERATION

Published on Jan 1, 2010
Xu Xu Bao3
Estimated H-index: 3
,
Akira Teramoto16
Estimated H-index: 16
+ 0 AuthorsKoji Abe17
Estimated H-index: 17
Source
Abstract
Bone is a natural composite mainly made from nano/micro-fibers of collagen and hydroxyapatite. In tissue-engineered bone regeneration, it is important to synthesize nano-composite scaffolds with good biocompatibility, high bioactivity, and strong bonding properties. In this study, a chitosan/poly(L-lactic acid) (CS/PLLA) blend of nano/micro fibers were prepared by using electrospinning, to form a three-dimensional porous scaffold for bone regeneration. FT-IR and tensile tests show that we succeed in fabricating CS/PLLA blend fibers with enhanced tensile properties, while maintaining the bioactive amino groups. Moreover, water contact angle and water uptake results show that chitosan content increases the hydrophilicity of the fibrous scaffold, which has positive effects on osteoblast adhesion. In cell proliferation, differentiation and mineralization assays using mice osteoblast-like (MC3T3-E1) cells, the CS/PLLA blends of 1:1 and 2:1 had almost identical bioactivities and were the most promising for bone regeneration.
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