Published on Jan 1, 2010
Xu Xu Bao3
Estimated H-index: 3
Akira Teramoto16
Estimated H-index: 16
+ 0 AuthorsKoji Abe17
Estimated H-index: 17
Bone is a natural composite mainly made from nano/micro-fibers of collagen and hydroxyapatite. In tissue-engineered bone regeneration, it is important to synthesize nano-composite scaffolds with good biocompatibility, high bioactivity, and strong bonding properties. In this study, a chitosan/poly(L-lactic acid) (CS/PLLA) blend of nano/micro fibers were prepared by using electrospinning, to form a three-dimensional porous scaffold for bone regeneration. FT-IR and tensile tests show that we succeed in fabricating CS/PLLA blend fibers with enhanced tensile properties, while maintaining the bioactive amino groups. Moreover, water contact angle and water uptake results show that chitosan content increases the hydrophilicity of the fibrous scaffold, which has positive effects on osteoblast adhesion. In cell proliferation, differentiation and mineralization assays using mice osteoblast-like (MC3T3-E1) cells, the CS/PLLA blends of 1:1 and 2:1 had almost identical bioactivities and were the most promising for bone regeneration.
📖 Papers frequently viewed together
2 Citations
3 Citations
5 Citations
#1Ying LuoH-Index: 18
#2George C. EngelmayrH-Index: 26
Last. Robert LangerH-Index: 296
view all 1 authors...
Abstract Three-dimensional (3D) scaffolds serve important functions in tissue engineering (TE) by providing physical and structural support for cells and delivering essential cues required for tissue formation/regeneration. With the advancements in understanding cell–material interactions and material fabrication techniques, the design of 3D scaffolds has evolved to generating functional and interactive environment to instruct and coordinate cellular activities and biological events at multiple ...
14 CitationsSource
#2Kwong-Joo LeckH-Index: 3
Last. Andrew C.A. WanH-Index: 32
view all 4 authors...
Abstract The extracellular matrix (ECM) is a rich meshwork of proteins and proteoglycans. Besides assuming a cell adhesive and structural support role, the ECM also helps to sequester and present growth factors to cells. ECM derived from tissues has been used as biological scaffolds for tissue engineering. In contrast, it has been difficult to employ ECM derived from cell lines as scaffolds due to its lack of form and structure. We have developed a mild, aqueous-based method for incorporating ce...
61 CitationsSource
#1João F. Mano (University of Minho)H-Index: 106
#2Graham Hungerford (University of Minho)H-Index: 22
Last. José Luis Gómez Ribelles (Polytechnic University of Valencia)H-Index: 25
view all 3 authors...
Poly(L-lactic acid)/Chitosan (PLLA/CHT) hybrid scaffolds were prepared by depositing CHT onto the pore walls in order to form a micro-structured low dense foam inside previously prepared PLLA scaffolds. The morphology and pore architecture of the scaffolds were evaluated by scanning electronic microscopy (SEM) and micro-computational tomography (micro-CT). The microstructure of the CHT fraction depends on its concentration in the solution used to impregnate the PLLA scaffolds. The water-uptake c...
57 CitationsSource
#1Wen Liang (ECUST: East China University of Science and Technology)H-Index: 9
#2Mohamed N. Rahaman (Missouri University of Science and Technology)H-Index: 69
Last. Jeremy J. Mao (UIC: University of Illinois at Chicago)H-Index: 66
view all 6 authors...
Abstract Borate glass particles and microspheres with size distributions in the range of approximately 100–400 μm, were loosely compacted and sintered for 10 min at 600 °C to form a porous, three-dimensional construct (porosity 25–40%). Conversion of the borate glass to hydroxyapatite was investigated by soaking the constructs in a solution of K 2 HPO 4 (0.25 M) at 37 °C and with a pH value of 9.0, and measuring the weight loss of the constructs as a function of time. Almost full conversion of t...
158 CitationsSource
#1Chengtie Wu (USYD: University of Sydney)H-Index: 80
#2Yogambha Ramaswamy (USYD: University of Sydney)H-Index: 19
Last. Hala Zreiqat (USYD: University of Sydney)H-Index: 55
view all 4 authors...
Porous calcium silicates (CaSiO3, WT) are regarded as a potential bioactive material for bone tissue engineering. However, their insufficient mechanical strength and high dissolution (degradation) limit their biological applications. The aim of this study is to surface modify WT scaffolds with poly(d,l-lactic acid) (PDLLA) to improve their mechanical and biological properties. The phase composition, microstructure, porosity and interconnectivity of WT and PDLLA-modified WT (WTPL) scaffolds were ...
153 CitationsSource
#1Hua Wu (Fujian Medical University)H-Index: 7
#2Ying Wan (HUST: Huazhong University of Science and Technology)H-Index: 32
Last. Quan Wu (RMC: Royal Military College of Canada)H-Index: 7
view all 4 authors...
Abstract Porous poly( dl -lactide)(PDLLA)/chitosan scaffolds with well-controlled pore structures and desirable mechanical characteristics were fabricated via a combination of solvent extraction, phase separation and freeze-drying. These scaffolds were further evaluated for the proliferation of isolated rabbit chondrocytes in vitro for various incubation periods up to 4 weeks in order to finally use them for the cartilage tissue engineering. MTT assay data revealed that the number of cells grown...
83 CitationsSource
#1Soo-Hong LeeH-Index: 32
#2Heungsoo Shin (Hanyang University)H-Index: 48
Last. Heungsoo Shin (Hanyang University)H-Index: 22
view all 2 authors...
Regeneration of bone and cartilage defects can be accelerated by localized delivery of appropriate growth factors incorporated within biodegradable carriers. The carrier essentially allows the impregnated growth factor to release at a desirable rate and concentration, and to linger at injury sites for a sufficient time to recruit progenitors and stimulate tissue healing processes. In addition, the carrier can be formulated to have particular structure to facilitate cellular infiltration and grow...
711 CitationsSource
#1Zhang Xiufang (Donghua University)H-Index: 1
#2Hui Hua (Donghua University)H-Index: 1
Last. Qing Yang (Donghua University)H-Index: 1
view all 4 authors...
Abstract In this study, poly( l -lactic acid) (PLLA) fibers were prepared by the dry–wet-spinning method, while chitosan (CHS) fibers were prepared via the wet-spinning method. The two fibers were blend spun and then fabricated into PLLA/CHS fabrics. In vitro degradation experiments of the fabrics were carried out in a phosphate-buffered solution at 37 °C with a pH of 7.4. Changes in molecular parameters (molecular weights and molecular weight distributions), phase structures (crystallinities), ...
98 CitationsSource
#1Aron Lazary (Semmelweis University)H-Index: 19
#2Bernadett Balla (Semmelweis University)H-Index: 14
Last. Peter L. Lakatos (Semmelweis University)H-Index: 82
view all 9 authors...
Recently, calcium sulfate dihydrate has been demonstrated as safe biodegradable osteoconductive bone void filler. However, its exact mechanism of action on bone cells is yet unknown. In this study, the influence of gypsum on gene expression and proliferation of MC3T3-E1 mouse pre-osteoblastic cells was investigated. Cells were cultured on gypsum disc, slice, polymethylmethacrylate (PMMA), or plastic culture plate for 15 days. Cell viability, alkaline phosphatase (ALP) activity and expression pro...
121 CitationsSource
#1Victor J. Chen (UM: University of Michigan)H-Index: 6
#2Laura A. Smith (UM: University of Michigan)H-Index: 10
Last. Peter X. Ma (UM: University of Michigan)H-Index: 100
view all 3 authors...
Abstract The ability to control architectural features in tissue engineering scaffolds is critical to the success of neo-tissue regeneration. In this work, reverse solid freeform fabrication and thermal phase separation of poly( l -lactic acid) (PLLA) solutions were used to create three-dimensional nano-fibrous (NF) scaffolds with complex geometries on the macro- and micro-scales. This approach allows for the fabrication of NF matrices while having precise control of internal pore size and struc...
216 CitationsSource
Cited By0