A new quantitative LC tandem mass spectrometry assay for serum 25-hydroxy vitamin D

The accurate measurement of 25-hydoxy vitamin D (25OH-D) in serum has been a challenge for many years. We developed a liquid chromatography tandem mass spectrometry (LC Tandem MS) assay for the quantitative determination of 25OH-D(2) and 25OH-D(3) in serum. The new method was compared with two widely used commercially available immunoassays.Sample preparation involved protein precipitation with acetonitrile containing deuterated forms of the...