Preparative isolation and separation of a novel and two known flavonoids from Patrinia villosa Juss by high-speed counter-current chromatography

Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and separation three flavonoids including bolusanthol B, a novel compound named 5,7,2′,6′-tetrahydroxy-6,8-di(γ,γ-dimethylallyl) flavanone and tetrapterol I from Patrinia villosa Juss using two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water at the volume ratio of 10:11:11:8 (v/v). A total of 25.4 mg bolusanthol B, 52.5 mg...