Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology
Abstract
We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity to support this claim, we used two new and complementary approaches to suppress exponential background DNA amplification that resulted from mispriming...
Paper Details
Title
Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology
Published Date
Feb 18, 2007
Journal
Volume
4
Issue
3
Pages
257 - 262
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Notes
History