The cell‐surface proteome of cultured adipose stromal cells

In this technical note we describe a method to evaluate the cell surface proteome of human primary cell cultures and cell lines. The method utilizes the BD Biosciences lyoplate, a system covering 242 surface proteins, glycoproteins, and glycosphingolipids plus relevant isotype controls, automated plate‐based flow cytometry, conventional file‐level analysis and unsupervised K‐means clustering of markers on the basis of percent of positive events...