Development and Preclinical Characterization of a Humanized Antibody Targeting CXCL12

Our goal was to develop a potent humanized antibody against mouse/human CXCL12. This report summarized its in vitro and in vivo activities.Cell surface binding and cell migration assays were used to select neutralizing hamster antibodies, followed by testing in several animal models. Monoclonal antibody (mAb) 30D8 was selected for humanization based on its in vitro and in vivo activities.30D8, a hamster antibody against mouse and human CXCL12α,...